Experiment: KLEB29 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10720 and BWI76_RS10725 are separated by 516 nucleotides BWI76_RS10725 and BWI76_RS10730 are separated by 139 nucleotides BWI76_RS10730 and BWI76_RS10735 are separated by 147 nucleotides BWI76_RS10735 and BWI76_RS10740 are separated by 19 nucleotides
BWI76_RS10720: BWI76_RS10720 - putrescine importer, at 2,265,191 to 2,266,582
_RS10720
BWI76_RS10725: BWI76_RS10725 - hypothetical protein, at 2,267,099 to 2,267,323
_RS10725
BWI76_RS10730: BWI76_RS10730 - bifunctional glucose-1-phosphatase/inositol phosphatase, at 2,267,463 to 2,268,710
_RS10730
BWI76_RS10735: BWI76_RS10735 - hypothetical protein, at 2,268,858 to 2,269,085
_RS10735
BWI76_RS10740: BWI76_RS10740 - NAD(P)H:quinone oxidoreductase, at 2,269,105 to 2,269,701
_RS10740
Position (kb)
2267
2268
2269 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2266.492 kb on - strand at 2266.506 kb on - strand at 2266.551 kb on - strand at 2266.567 kb on + strand at 2266.567 kb on + strand at 2266.570 kb on - strand at 2266.598 kb on + strand at 2266.739 kb on - strand at 2266.776 kb on + strand at 2266.776 kb on + strand at 2267.058 kb on + strand at 2267.064 kb on + strand at 2267.064 kb on + strand at 2267.066 kb on - strand at 2267.148 kb on - strand, within BWI76_RS10725 at 2267.264 kb on - strand, within BWI76_RS10725 at 2267.502 kb on + strand at 2267.510 kb on - strand at 2267.535 kb on - strand at 2267.592 kb on - strand at 2267.728 kb on - strand, within BWI76_RS10730 at 2267.861 kb on - strand, within BWI76_RS10730 at 2268.911 kb on - strand, within BWI76_RS10735 at 2269.145 kb on + strand at 2269.145 kb on + strand at 2269.610 kb on - strand, within BWI76_RS10740 at 2269.634 kb on - strand, within BWI76_RS10740 at 2269.652 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB29 MOI 1 remove 2,266,492 - -1.9 2,266,506 - -2.9 2,266,551 - +0.2 2,266,567 + -1.0 2,266,567 + +0.6 2,266,570 - +2.1 2,266,598 + -1.2 2,266,739 - -0.9 2,266,776 + +0.7 2,266,776 + +0.6 2,267,058 + -1.9 2,267,064 + -2.2 2,267,064 + -2.2 2,267,066 - +2.5 2,267,148 - BWI76_RS10725 0.22 +0.6 2,267,264 - BWI76_RS10725 0.73 -0.3 2,267,502 + +0.6 2,267,510 - -0.9 2,267,535 - +3.2 2,267,592 - +2.9 2,267,728 - BWI76_RS10730 0.21 -1.4 2,267,861 - BWI76_RS10730 0.32 -0.8 2,268,911 - BWI76_RS10735 0.23 +0.3 2,269,145 + +3.1 2,269,145 + -1.6 2,269,610 - BWI76_RS10740 0.85 -0.5 2,269,634 - BWI76_RS10740 0.89 +0.7 2,269,652 + +0.2
Or see this region's nucleotide sequence