Experiment: KLEB29 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS09810 and BWI76_RS09815 overlap by 4 nucleotides BWI76_RS09815 and BWI76_RS09820 are separated by 141 nucleotides BWI76_RS09820 and BWI76_RS09825 overlap by 24 nucleotides BWI76_RS09825 and BWI76_RS09830 are separated by 121 nucleotides
BWI76_RS09810: BWI76_RS09810 - hypothetical protein, at 2,059,189 to 2,059,371
_RS09810
BWI76_RS09815: BWI76_RS09815 - 3-deoxy-manno-octulosonate cytidylyltransferase, at 2,059,368 to 2,060,114
_RS09815
BWI76_RS09820: BWI76_RS09820 - hypothetical protein, at 2,060,256 to 2,061,149
_RS09820
BWI76_RS09825: BWI76_RS09825 - envelope biogenesis factor ElyC, at 2,061,126 to 2,061,905
_RS09825
BWI76_RS09830: BWI76_RS09830 - tRNA uridine 5-oxyacetic acid(34) methyltransferase CmoM, at 2,062,027 to 2,062,806
_RS09830
Position (kb)
2060
2061
2062 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2060.371 kb on + strand, within BWI76_RS09820 at 2060.689 kb on - strand, within BWI76_RS09820 at 2060.851 kb on + strand, within BWI76_RS09820 at 2060.870 kb on + strand, within BWI76_RS09820 at 2060.915 kb on + strand, within BWI76_RS09820 at 2061.255 kb on - strand, within BWI76_RS09825 at 2061.333 kb on + strand, within BWI76_RS09825 at 2061.806 kb on + strand, within BWI76_RS09825 at 2061.811 kb on + strand, within BWI76_RS09825 at 2061.814 kb on - strand, within BWI76_RS09825 at 2061.990 kb on - strand at 2062.032 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB29 MOI 1 remove 2,060,371 + BWI76_RS09820 0.13 +2.7 2,060,689 - BWI76_RS09820 0.48 +1.1 2,060,851 + BWI76_RS09820 0.67 +0.3 2,060,870 + BWI76_RS09820 0.69 -2.0 2,060,915 + BWI76_RS09820 0.74 +0.5 2,061,255 - BWI76_RS09825 0.17 -0.3 2,061,333 + BWI76_RS09825 0.27 +1.4 2,061,806 + BWI76_RS09825 0.87 +1.6 2,061,811 + BWI76_RS09825 0.88 +2.0 2,061,814 - BWI76_RS09825 0.88 +1.5 2,061,990 - +2.9 2,062,032 - +0.6
Or see this region's nucleotide sequence