Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS09110

Experiment: KLEB29 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS09100 and BWI76_RS09105 are separated by 52 nucleotidesBWI76_RS09105 and BWI76_RS09110 are separated by 1 nucleotidesBWI76_RS09110 and BWI76_RS09115 are separated by 182 nucleotides BWI76_RS09100: BWI76_RS09100 - glutathione ABC transporter substrate-binding protein GsiB, at 1,922,108 to 1,923,646 _RS09100 BWI76_RS09105: BWI76_RS09105 - glutathione ABC transporter permease GsiC, at 1,923,699 to 1,924,619 _RS09105 BWI76_RS09110: BWI76_RS09110 - glutathione ABC transporter permease GsiD, at 1,924,621 to 1,925,532 _RS09110 BWI76_RS09115: BWI76_RS09115 - 30S ribosomal protein S12 methylthiotransferase RimO, at 1,925,715 to 1,927,040 _RS09115 Position (kb) 1924 1925 1926Strain fitness (log2 ratio) -2 -1 0 1at 1923.992 kb on + strand, within BWI76_RS09105at 1924.096 kb on - strand, within BWI76_RS09105at 1924.452 kb on + strand, within BWI76_RS09105at 1924.671 kb on + strandat 1924.681 kb on + strandat 1924.754 kb on + strand, within BWI76_RS09110at 1924.779 kb on + strand, within BWI76_RS09110at 1924.996 kb on + strand, within BWI76_RS09110at 1925.639 kb on + strandat 1925.647 kb on - strandat 1925.647 kb on - strandat 1925.654 kb on + strandat 1926.086 kb on - strand, within BWI76_RS09115at 1926.161 kb on + strandat 1926.194 kb on + strand, within BWI76_RS09115at 1926.377 kb on + strand, within BWI76_RS09115

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB29 MOI 1
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1,923,992 + BWI76_RS09105 0.32 -0.5
1,924,096 - BWI76_RS09105 0.43 +0.5
1,924,452 + BWI76_RS09105 0.82 +0.3
1,924,671 + -0.1
1,924,681 + +1.7
1,924,754 + BWI76_RS09110 0.15 +0.5
1,924,779 + BWI76_RS09110 0.17 -0.4
1,924,996 + BWI76_RS09110 0.41 -2.1
1,925,639 + -0.1
1,925,647 - -0.8
1,925,647 - -0.9
1,925,654 + -0.2
1,926,086 - BWI76_RS09115 0.28 -0.7
1,926,161 + -1.5
1,926,194 + BWI76_RS09115 0.36 -0.7
1,926,377 + BWI76_RS09115 0.50 -0.1

Or see this region's nucleotide sequence