Experiment: KLEB29 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS08345 and BWI76_RS08350 are separated by 15 nucleotides BWI76_RS08350 and BWI76_RS08355 overlap by 7 nucleotides BWI76_RS08355 and BWI76_RS08360 overlap by 11 nucleotides BWI76_RS08360 and BWI76_RS08365 are separated by 62 nucleotides
BWI76_RS08345: BWI76_RS08345 - Nif3-like dinuclear metal center protein, at 1,767,314 to 1,768,057
_RS08345
BWI76_RS08350: BWI76_RS08350 - carboxylase, at 1,768,073 to 1,768,729
_RS08350
BWI76_RS08355: BWI76_RS08355 - hypothetical protein, at 1,768,723 to 1,769,655
_RS08355
BWI76_RS08360: BWI76_RS08360 - LamB/YcsF family protein, at 1,769,645 to 1,770,391
_RS08360
BWI76_RS08365: BWI76_RS08365 - membrane protein, at 1,770,454 to 1,771,179
_RS08365
Position (kb)
1768
1769
1770 Strain fitness (log2 ratio)
-1
0
1
2 at 1767.826 kb on - strand, within BWI76_RS08345 at 1767.844 kb on - strand, within BWI76_RS08345 at 1768.248 kb on + strand, within BWI76_RS08350 at 1768.282 kb on + strand, within BWI76_RS08350 at 1768.293 kb on + strand, within BWI76_RS08350 at 1768.767 kb on - strand at 1768.889 kb on + strand, within BWI76_RS08355 at 1768.985 kb on - strand, within BWI76_RS08355 at 1769.103 kb on + strand, within BWI76_RS08355 at 1769.213 kb on + strand, within BWI76_RS08355 at 1769.221 kb on - strand, within BWI76_RS08355 at 1769.373 kb on - strand, within BWI76_RS08355 at 1769.610 kb on + strand at 1769.791 kb on - strand, within BWI76_RS08360 at 1770.049 kb on - strand, within BWI76_RS08360 at 1770.217 kb on - strand, within BWI76_RS08360 at 1770.298 kb on + strand, within BWI76_RS08360
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB29 MOI 1 remove 1,767,826 - BWI76_RS08345 0.69 +0.9 1,767,844 - BWI76_RS08345 0.71 -0.4 1,768,248 + BWI76_RS08350 0.27 +1.2 1,768,282 + BWI76_RS08350 0.32 -0.3 1,768,293 + BWI76_RS08350 0.33 +2.6 1,768,767 - +0.2 1,768,889 + BWI76_RS08355 0.18 +1.1 1,768,985 - BWI76_RS08355 0.28 -0.2 1,769,103 + BWI76_RS08355 0.41 +0.7 1,769,213 + BWI76_RS08355 0.53 +1.2 1,769,221 - BWI76_RS08355 0.53 +0.4 1,769,373 - BWI76_RS08355 0.70 +0.2 1,769,610 + +1.3 1,769,791 - BWI76_RS08360 0.20 -0.8 1,770,049 - BWI76_RS08360 0.54 -1.2 1,770,217 - BWI76_RS08360 0.77 +2.7 1,770,298 + BWI76_RS08360 0.87 -1.7
Or see this region's nucleotide sequence