Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS07630

Experiment: KLEB29 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS07615 and BWI76_RS07620 are separated by 10 nucleotidesBWI76_RS07620 and BWI76_RS07625 overlap by 84 nucleotidesBWI76_RS07625 and BWI76_RS07630 overlap by 4 nucleotidesBWI76_RS07630 and BWI76_RS07635 are separated by 222 nucleotidesBWI76_RS07635 and BWI76_RS07640 are separated by 193 nucleotides BWI76_RS07615: BWI76_RS07615 - betaine-aldehyde dehydrogenase, at 1,613,138 to 1,614,610 _RS07615 BWI76_RS07620: BWI76_RS07620 - transcriptional regulator BetI, at 1,614,621 to 1,615,214 _RS07620 BWI76_RS07625: BWI76_RS07625 - hypothetical protein, at 1,615,131 to 1,615,346 _RS07625 BWI76_RS07630: BWI76_RS07630 - high-affinity choline transporter BetT, at 1,615,343 to 1,617,376 _RS07630 BWI76_RS07635: BWI76_RS07635 - Hok/gef cell toxic protein, at 1,617,599 to 1,617,751 _RS07635 BWI76_RS07640: BWI76_RS07640 - short chain dehydrogenase, at 1,617,945 to 1,618,730 _RS07640 Position (kb) 1615 1616 1617 1618Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 1614.458 kb on + strand, within BWI76_RS07615at 1614.501 kb on + strandat 1614.501 kb on + strandat 1614.605 kb on + strandat 1614.702 kb on + strand, within BWI76_RS07620at 1614.818 kb on + strand, within BWI76_RS07620at 1615.001 kb on - strand, within BWI76_RS07620at 1615.039 kb on + strand, within BWI76_RS07620at 1615.467 kb on + strandat 1615.478 kb on + strandat 1615.552 kb on + strand, within BWI76_RS07630at 1615.590 kb on + strandat 1615.730 kb on - strand, within BWI76_RS07630at 1616.014 kb on + strandat 1616.151 kb on + strand, within BWI76_RS07630at 1616.170 kb on + strand, within BWI76_RS07630at 1616.186 kb on - strand, within BWI76_RS07630at 1616.421 kb on + strand, within BWI76_RS07630at 1616.562 kb on + strand, within BWI76_RS07630at 1616.686 kb on - strand, within BWI76_RS07630at 1616.963 kb on + strand, within BWI76_RS07630at 1617.084 kb on + strand, within BWI76_RS07630at 1617.371 kb on + strandat 1617.637 kb on + strand, within BWI76_RS07635at 1617.647 kb on - strand, within BWI76_RS07635at 1617.647 kb on - strand, within BWI76_RS07635at 1617.660 kb on + strandat 1617.668 kb on - strand, within BWI76_RS07635at 1617.668 kb on - strand, within BWI76_RS07635at 1617.800 kb on + strandat 1617.807 kb on - strandat 1617.890 kb on + strandat 1617.890 kb on - strandat 1618.235 kb on - strand, within BWI76_RS07640at 1618.235 kb on - strand, within BWI76_RS07640at 1618.272 kb on + strand, within BWI76_RS07640

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB29 MOI 1
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1,614,458 + BWI76_RS07615 0.90 -0.2
1,614,501 + +1.8
1,614,501 + +0.1
1,614,605 + +0.4
1,614,702 + BWI76_RS07620 0.14 +0.0
1,614,818 + BWI76_RS07620 0.33 -0.1
1,615,001 - BWI76_RS07620 0.64 -0.1
1,615,039 + BWI76_RS07620 0.70 -0.6
1,615,467 + +3.8
1,615,478 + +0.4
1,615,552 + BWI76_RS07630 0.10 -1.4
1,615,590 + -0.6
1,615,730 - BWI76_RS07630 0.19 -0.4
1,616,014 + +1.0
1,616,151 + BWI76_RS07630 0.40 -3.0
1,616,170 + BWI76_RS07630 0.41 -2.2
1,616,186 - BWI76_RS07630 0.41 -1.5
1,616,421 + BWI76_RS07630 0.53 -1.6
1,616,562 + BWI76_RS07630 0.60 -1.9
1,616,686 - BWI76_RS07630 0.66 -2.7
1,616,963 + BWI76_RS07630 0.80 -0.0
1,617,084 + BWI76_RS07630 0.86 -1.3
1,617,371 + -0.7
1,617,637 + BWI76_RS07635 0.25 -0.4
1,617,647 - BWI76_RS07635 0.31 -0.7
1,617,647 - BWI76_RS07635 0.31 +2.0
1,617,660 + +1.0
1,617,668 - BWI76_RS07635 0.45 -0.7
1,617,668 - BWI76_RS07635 0.45 -0.2
1,617,800 + -1.2
1,617,807 - -0.1
1,617,890 + -1.1
1,617,890 - -1.7
1,618,235 - BWI76_RS07640 0.37 -1.0
1,618,235 - BWI76_RS07640 0.37 +2.4
1,618,272 + BWI76_RS07640 0.42 +1.3

Or see this region's nucleotide sequence