Experiment: KLEB29 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS07615 and BWI76_RS07620 are separated by 10 nucleotides BWI76_RS07620 and BWI76_RS07625 overlap by 84 nucleotides BWI76_RS07625 and BWI76_RS07630 overlap by 4 nucleotides BWI76_RS07630 and BWI76_RS07635 are separated by 222 nucleotides BWI76_RS07635 and BWI76_RS07640 are separated by 193 nucleotides
BWI76_RS07615: BWI76_RS07615 - betaine-aldehyde dehydrogenase, at 1,613,138 to 1,614,610
_RS07615
BWI76_RS07620: BWI76_RS07620 - transcriptional regulator BetI, at 1,614,621 to 1,615,214
_RS07620
BWI76_RS07625: BWI76_RS07625 - hypothetical protein, at 1,615,131 to 1,615,346
_RS07625
BWI76_RS07630: BWI76_RS07630 - high-affinity choline transporter BetT, at 1,615,343 to 1,617,376
_RS07630
BWI76_RS07635: BWI76_RS07635 - Hok/gef cell toxic protein, at 1,617,599 to 1,617,751
_RS07635
BWI76_RS07640: BWI76_RS07640 - short chain dehydrogenase, at 1,617,945 to 1,618,730
_RS07640
Position (kb)
1615
1616
1617
1618 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1614.458 kb on + strand, within BWI76_RS07615 at 1614.501 kb on + strand at 1614.501 kb on + strand at 1614.605 kb on + strand at 1614.702 kb on + strand, within BWI76_RS07620 at 1614.818 kb on + strand, within BWI76_RS07620 at 1615.001 kb on - strand, within BWI76_RS07620 at 1615.039 kb on + strand, within BWI76_RS07620 at 1615.467 kb on + strand at 1615.478 kb on + strand at 1615.552 kb on + strand, within BWI76_RS07630 at 1615.590 kb on + strand at 1615.730 kb on - strand, within BWI76_RS07630 at 1616.014 kb on + strand at 1616.151 kb on + strand, within BWI76_RS07630 at 1616.170 kb on + strand, within BWI76_RS07630 at 1616.186 kb on - strand, within BWI76_RS07630 at 1616.421 kb on + strand, within BWI76_RS07630 at 1616.562 kb on + strand, within BWI76_RS07630 at 1616.686 kb on - strand, within BWI76_RS07630 at 1616.963 kb on + strand, within BWI76_RS07630 at 1617.084 kb on + strand, within BWI76_RS07630 at 1617.371 kb on + strand at 1617.637 kb on + strand, within BWI76_RS07635 at 1617.647 kb on - strand, within BWI76_RS07635 at 1617.647 kb on - strand, within BWI76_RS07635 at 1617.660 kb on + strand at 1617.668 kb on - strand, within BWI76_RS07635 at 1617.668 kb on - strand, within BWI76_RS07635 at 1617.800 kb on + strand at 1617.807 kb on - strand at 1617.890 kb on + strand at 1617.890 kb on - strand at 1618.235 kb on - strand, within BWI76_RS07640 at 1618.235 kb on - strand, within BWI76_RS07640 at 1618.272 kb on + strand, within BWI76_RS07640
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB29 MOI 1 remove 1,614,458 + BWI76_RS07615 0.90 -0.2 1,614,501 + +1.8 1,614,501 + +0.1 1,614,605 + +0.4 1,614,702 + BWI76_RS07620 0.14 +0.0 1,614,818 + BWI76_RS07620 0.33 -0.1 1,615,001 - BWI76_RS07620 0.64 -0.1 1,615,039 + BWI76_RS07620 0.70 -0.6 1,615,467 + +3.8 1,615,478 + +0.4 1,615,552 + BWI76_RS07630 0.10 -1.4 1,615,590 + -0.6 1,615,730 - BWI76_RS07630 0.19 -0.4 1,616,014 + +1.0 1,616,151 + BWI76_RS07630 0.40 -3.0 1,616,170 + BWI76_RS07630 0.41 -2.2 1,616,186 - BWI76_RS07630 0.41 -1.5 1,616,421 + BWI76_RS07630 0.53 -1.6 1,616,562 + BWI76_RS07630 0.60 -1.9 1,616,686 - BWI76_RS07630 0.66 -2.7 1,616,963 + BWI76_RS07630 0.80 -0.0 1,617,084 + BWI76_RS07630 0.86 -1.3 1,617,371 + -0.7 1,617,637 + BWI76_RS07635 0.25 -0.4 1,617,647 - BWI76_RS07635 0.31 -0.7 1,617,647 - BWI76_RS07635 0.31 +2.0 1,617,660 + +1.0 1,617,668 - BWI76_RS07635 0.45 -0.7 1,617,668 - BWI76_RS07635 0.45 -0.2 1,617,800 + -1.2 1,617,807 - -0.1 1,617,890 + -1.1 1,617,890 - -1.7 1,618,235 - BWI76_RS07640 0.37 -1.0 1,618,235 - BWI76_RS07640 0.37 +2.4 1,618,272 + BWI76_RS07640 0.42 +1.3
Or see this region's nucleotide sequence