Experiment: KLEB29 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05975 and BWI76_RS05980 overlap by 8 nucleotides BWI76_RS05980 and BWI76_RS05985 overlap by 14 nucleotides BWI76_RS05985 and BWI76_RS05990 overlap by 1 nucleotides BWI76_RS05990 and BWI76_RS05995 are separated by 20 nucleotides
BWI76_RS05975: BWI76_RS05975 - ABC transporter ATP-binding protein, at 1,254,035 to 1,254,730
_RS05975
BWI76_RS05980: BWI76_RS05980 - ABC transporter ATP-binding protein, at 1,254,723 to 1,255,505
_RS05980
BWI76_RS05985: BWI76_RS05985 - branched-chain amino acid ABC transporter permease, at 1,255,492 to 1,256,541
_RS05985
BWI76_RS05990: BWI76_RS05990 - branched-chain amino acid ABC transporter permease, at 1,256,541 to 1,257,440
_RS05990
BWI76_RS05995: BWI76_RS05995 - branched-chain amino acid ABC transporter substrate-binding protein, at 1,257,461 to 1,258,597
_RS05995
Position (kb)
1255
1256
1257 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1254.675 kb on + strand at 1254.794 kb on - strand at 1254.816 kb on - strand, within BWI76_RS05980 at 1254.856 kb on + strand, within BWI76_RS05980 at 1255.055 kb on - strand, within BWI76_RS05980 at 1255.099 kb on + strand, within BWI76_RS05980 at 1255.099 kb on + strand, within BWI76_RS05980 at 1255.182 kb on + strand, within BWI76_RS05980 at 1255.338 kb on - strand, within BWI76_RS05980 at 1255.352 kb on + strand at 1255.384 kb on + strand, within BWI76_RS05980 at 1255.465 kb on + strand at 1255.831 kb on + strand, within BWI76_RS05985 at 1256.049 kb on - strand, within BWI76_RS05985 at 1256.193 kb on + strand, within BWI76_RS05985 at 1256.366 kb on - strand, within BWI76_RS05985 at 1256.382 kb on - strand, within BWI76_RS05985 at 1256.441 kb on - strand at 1256.501 kb on - strand at 1256.713 kb on - strand, within BWI76_RS05990 at 1256.756 kb on + strand, within BWI76_RS05990 at 1256.775 kb on + strand, within BWI76_RS05990 at 1256.786 kb on + strand, within BWI76_RS05990 at 1256.809 kb on + strand, within BWI76_RS05990 at 1256.809 kb on + strand, within BWI76_RS05990 at 1256.889 kb on + strand, within BWI76_RS05990 at 1257.244 kb on - strand, within BWI76_RS05990 at 1257.336 kb on + strand, within BWI76_RS05990 at 1257.396 kb on - strand at 1257.524 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB29 MOI 1 remove 1,254,675 + -2.7 1,254,794 - +1.3 1,254,816 - BWI76_RS05980 0.12 -0.4 1,254,856 + BWI76_RS05980 0.17 -1.2 1,255,055 - BWI76_RS05980 0.42 -1.6 1,255,099 + BWI76_RS05980 0.48 +2.0 1,255,099 + BWI76_RS05980 0.48 -2.4 1,255,182 + BWI76_RS05980 0.59 -0.3 1,255,338 - BWI76_RS05980 0.79 -2.2 1,255,352 + +1.8 1,255,384 + BWI76_RS05980 0.84 +0.7 1,255,465 + -0.4 1,255,831 + BWI76_RS05985 0.32 -1.1 1,256,049 - BWI76_RS05985 0.53 -0.4 1,256,193 + BWI76_RS05985 0.67 -1.2 1,256,366 - BWI76_RS05985 0.83 -0.0 1,256,382 - BWI76_RS05985 0.85 -2.0 1,256,441 - -0.3 1,256,501 - -1.5 1,256,713 - BWI76_RS05990 0.19 -4.0 1,256,756 + BWI76_RS05990 0.24 -0.2 1,256,775 + BWI76_RS05990 0.26 -2.3 1,256,786 + BWI76_RS05990 0.27 -0.8 1,256,809 + BWI76_RS05990 0.30 -0.8 1,256,809 + BWI76_RS05990 0.30 -1.1 1,256,889 + BWI76_RS05990 0.39 -0.7 1,257,244 - BWI76_RS05990 0.78 -0.4 1,257,336 + BWI76_RS05990 0.88 -1.8 1,257,396 - -2.0 1,257,524 + -1.9
Or see this region's nucleotide sequence