Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS04340

Experiment: KLEB29 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS04330 and BWI76_RS04335 overlap by 24 nucleotidesBWI76_RS04335 and BWI76_RS04340 are separated by 44 nucleotidesBWI76_RS04340 and BWI76_RS04345 overlap by 1 nucleotidesBWI76_RS04345 and BWI76_RS04350 are separated by 54 nucleotides BWI76_RS04330: BWI76_RS04330 - hypothetical protein, at 891,854 to 892,088 _RS04330 BWI76_RS04335: BWI76_RS04335 - bifunctional riboflavin kinase/FMN adenylyltransferase, at 892,065 to 893,003 _RS04335 BWI76_RS04340: BWI76_RS04340 - isoleucine--tRNA ligase, at 893,048 to 895,864 _RS04340 BWI76_RS04345: BWI76_RS04345 - signal peptidase II, at 895,864 to 896,364 _RS04345 BWI76_RS04350: BWI76_RS04350 - FKBP-type peptidyl-prolyl cis-trans isomerase, at 896,419 to 896,868 _RS04350 Position (kb) 893 894 895 896Strain fitness (log2 ratio) -1 0 1 2at 896.421 kb on + strandat 896.558 kb on + strand, within BWI76_RS04350

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB29 MOI 1
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896,421 + +2.3
896,558 + BWI76_RS04350 0.31 +0.4

Or see this region's nucleotide sequence