Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS16870

Experiment: KLEB29 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS16865 and BWI76_RS16870 are separated by 145 nucleotidesBWI76_RS16870 and BWI76_RS16875 are separated by 107 nucleotidesBWI76_RS16875 and BWI76_RS16880 are separated by 1 nucleotides BWI76_RS16865: BWI76_RS16865 - coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase-related flavin-dependent oxidoreductase, at 3,497,494 to 3,498,579 _RS16865 BWI76_RS16870: BWI76_RS16870 - LysR family transcriptional regulator, at 3,498,725 to 3,499,618 _RS16870 BWI76_RS16875: BWI76_RS16875 - putative type I cysteine dioxygenase, at 3,499,726 to 3,500,322 _RS16875 BWI76_RS16880: BWI76_RS16880 - GlpE family protein, at 3,500,324 to 3,501,904 _RS16880 Position (kb) 3498 3499 3500Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 3497.738 kb on + strand, within BWI76_RS16865at 3498.029 kb on + strand, within BWI76_RS16865at 3498.154 kb on + strand, within BWI76_RS16865at 3498.173 kb on + strandat 3498.181 kb on - strand, within BWI76_RS16865at 3498.647 kb on - strandat 3498.975 kb on + strand, within BWI76_RS16870at 3498.976 kb on - strand, within BWI76_RS16870at 3499.376 kb on - strand, within BWI76_RS16870at 3499.812 kb on + strand, within BWI76_RS16875at 3499.991 kb on + strand, within BWI76_RS16875at 3500.040 kb on - strand, within BWI76_RS16875at 3500.097 kb on + strand, within BWI76_RS16875at 3500.156 kb on + strand, within BWI76_RS16875at 3500.170 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB29 MOI 1
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3,497,738 + BWI76_RS16865 0.22 +0.5
3,498,029 + BWI76_RS16865 0.49 -1.7
3,498,154 + BWI76_RS16865 0.61 -1.5
3,498,173 + +1.2
3,498,181 - BWI76_RS16865 0.63 +0.4
3,498,647 - -0.2
3,498,975 + BWI76_RS16870 0.28 -2.8
3,498,976 - BWI76_RS16870 0.28 -2.5
3,499,376 - BWI76_RS16870 0.73 -1.3
3,499,812 + BWI76_RS16875 0.14 -1.2
3,499,991 + BWI76_RS16875 0.44 -0.7
3,500,040 - BWI76_RS16875 0.53 -1.3
3,500,097 + BWI76_RS16875 0.62 -1.2
3,500,156 + BWI76_RS16875 0.72 +0.9
3,500,170 - +2.2

Or see this region's nucleotide sequence