Experiment: KLEB29 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14965 and BWI76_RS14970 overlap by 4 nucleotides BWI76_RS14970 and BWI76_RS14975 are separated by 12 nucleotides BWI76_RS14975 and BWI76_RS14980 overlap by 4 nucleotides BWI76_RS14980 and BWI76_RS14985 are separated by 4 nucleotides
BWI76_RS14965: BWI76_RS14965 - AraC family transcriptional regulator, at 3,137,120 to 3,138,076
_RS14965
BWI76_RS14970: BWI76_RS14970 - pyrroline-5-carboxylate reductase, at 3,138,073 to 3,138,861
_RS14970
BWI76_RS14975: BWI76_RS14975 - glyoxalase-like domain protein, at 3,138,874 to 3,139,629
_RS14975
BWI76_RS14980: BWI76_RS14980 - hypothetical protein, at 3,139,626 to 3,140,156
_RS14980
BWI76_RS14985: BWI76_RS14985 - amino acid ABC transporter substrate-binding protein, at 3,140,161 to 3,141,003
_RS14985
Position (kb)
3138
3139
3140 Strain fitness (log2 ratio)
-2
-1
0
1 at 3137.932 kb on - strand, within BWI76_RS14965 at 3137.985 kb on - strand at 3138.616 kb on - strand, within BWI76_RS14970 at 3139.204 kb on + strand, within BWI76_RS14975 at 3139.430 kb on + strand, within BWI76_RS14975 at 3139.477 kb on + strand, within BWI76_RS14975 at 3139.643 kb on + strand at 3140.090 kb on + strand, within BWI76_RS14980 at 3140.098 kb on - strand, within BWI76_RS14980 at 3140.122 kb on + strand at 3140.174 kb on + strand at 3140.207 kb on + strand at 3140.210 kb on - strand at 3140.222 kb on - strand at 3140.285 kb on - strand, within BWI76_RS14985 at 3140.558 kb on + strand, within BWI76_RS14985 at 3140.593 kb on - strand, within BWI76_RS14985
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB29 MOI 1 remove 3,137,932 - BWI76_RS14965 0.85 -0.3 3,137,985 - -0.2 3,138,616 - BWI76_RS14970 0.69 +0.1 3,139,204 + BWI76_RS14975 0.44 -0.1 3,139,430 + BWI76_RS14975 0.74 -1.1 3,139,477 + BWI76_RS14975 0.80 -1.0 3,139,643 + -1.6 3,140,090 + BWI76_RS14980 0.87 +0.8 3,140,098 - BWI76_RS14980 0.89 +0.5 3,140,122 + +1.5 3,140,174 + -0.7 3,140,207 + -2.0 3,140,210 - -1.2 3,140,222 - +0.6 3,140,285 - BWI76_RS14985 0.15 -0.4 3,140,558 + BWI76_RS14985 0.47 -0.5 3,140,593 - BWI76_RS14985 0.51 -2.3
Or see this region's nucleotide sequence