Experiment: KLEB29 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12935 and BWI76_RS12940 are separated by 77 nucleotides BWI76_RS12940 and BWI76_RS12945 are separated by 57 nucleotides BWI76_RS12945 and BWI76_RS12950 are separated by 334 nucleotides
BWI76_RS12935: BWI76_RS12935 - fusaric acid resistance protein, at 2,693,919 to 2,695,958
_RS12935
BWI76_RS12940: BWI76_RS12940 - AraC family transcriptional regulator, at 2,696,036 to 2,696,857
_RS12940
BWI76_RS12945: BWI76_RS12945 - AraC family transcriptional regulator, at 2,696,915 to 2,697,310
_RS12945
BWI76_RS12950: BWI76_RS12950 - acriflavin resistance protein AcrA, at 2,697,645 to 2,698,820
_RS12950
Position (kb)
2696
2697 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 2695.368 kb on - strand, within BWI76_RS12935 at 2695.368 kb on - strand, within BWI76_RS12935 at 2695.485 kb on + strand, within BWI76_RS12935 at 2695.493 kb on - strand, within BWI76_RS12935 at 2695.854 kb on + strand at 2695.885 kb on + strand at 2695.885 kb on + strand at 2696.060 kb on - strand at 2696.181 kb on + strand, within BWI76_RS12940 at 2696.414 kb on + strand, within BWI76_RS12940 at 2696.777 kb on - strand at 2696.859 kb on - strand at 2696.859 kb on - strand at 2697.835 kb on - strand, within BWI76_RS12950
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB29 MOI 1 remove 2,695,368 - BWI76_RS12935 0.71 -0.3 2,695,368 - BWI76_RS12935 0.71 +0.1 2,695,485 + BWI76_RS12935 0.77 +0.0 2,695,493 - BWI76_RS12935 0.77 +0.1 2,695,854 + +1.6 2,695,885 + +0.5 2,695,885 + -0.5 2,696,060 - +0.3 2,696,181 + BWI76_RS12940 0.18 -0.7 2,696,414 + BWI76_RS12940 0.46 -2.0 2,696,777 - -1.4 2,696,859 - -1.1 2,696,859 - -2.5 2,697,835 - BWI76_RS12950 0.16 +4.9
Or see this region's nucleotide sequence