Experiment: KLEB29 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS09125 and BWI76_RS09130 are separated by 376 nucleotides BWI76_RS09130 and BWI76_RS09135 are separated by 104 nucleotides BWI76_RS09135 and BWI76_RS09140 are separated by 2 nucleotides BWI76_RS09140 and BWI76_RS09145 are separated by 257 nucleotides
BWI76_RS09125: BWI76_RS09125 - MFS transporter, at 1,927,852 to 1,929,096
_RS09125
BWI76_RS09130: BWI76_RS09130 - biofilm formation regulatory protein BssR, at 1,929,473 to 1,929,856
_RS09130
BWI76_RS09135: BWI76_RS09135 - oxidoreductase, at 1,929,961 to 1,931,073
_RS09135
BWI76_RS09140: BWI76_RS09140 - glutathione S-transferase, at 1,931,076 to 1,931,702
_RS09140
BWI76_RS09145: BWI76_RS09145 - serine-type D-Ala-D-Ala carboxypeptidase, at 1,931,960 to 1,933,162
_RS09145
Position (kb) 1929
1930
1931
1932 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 1929.039 kb on - strand at 1929.105 kb on + strand at 1929.126 kb on - strand at 1929.476 kb on + strand at 1929.495 kb on - strand at 1929.619 kb on + strand, within BWI76_RS09130 at 1929.740 kb on + strand, within BWI76_RS09130 at 1929.748 kb on - strand, within BWI76_RS09130 at 1929.929 kb on - strand at 1930.520 kb on - strand, within BWI76_RS09135 at 1930.603 kb on + strand, within BWI76_RS09135 at 1930.603 kb on + strand, within BWI76_RS09135 at 1930.611 kb on - strand, within BWI76_RS09135 at 1930.730 kb on + strand, within BWI76_RS09135 at 1931.042 kb on - strand at 1931.229 kb on + strand, within BWI76_RS09140 at 1931.268 kb on + strand, within BWI76_RS09140 at 1931.352 kb on - strand, within BWI76_RS09140 at 1931.352 kb on - strand, within BWI76_RS09140 at 1931.400 kb on - strand, within BWI76_RS09140 at 1931.881 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB29 MOI 1 remove 1,929,039 - -2.7 1,929,105 + -0.0 1,929,126 - -0.7 1,929,476 + +0.4 1,929,495 - -0.2 1,929,619 + BWI76_RS09130 0.38 +1.2 1,929,740 + BWI76_RS09130 0.70 -0.5 1,929,748 - BWI76_RS09130 0.72 -1.0 1,929,929 - -0.3 1,930,520 - BWI76_RS09135 0.50 -0.7 1,930,603 + BWI76_RS09135 0.58 -1.4 1,930,603 + BWI76_RS09135 0.58 -3.6 1,930,611 - BWI76_RS09135 0.58 +0.3 1,930,730 + BWI76_RS09135 0.69 -0.9 1,931,042 - +0.8 1,931,229 + BWI76_RS09140 0.24 -0.1 1,931,268 + BWI76_RS09140 0.31 -0.4 1,931,352 - BWI76_RS09140 0.44 -2.1 1,931,352 - BWI76_RS09140 0.44 -0.2 1,931,400 - BWI76_RS09140 0.52 -0.1 1,931,881 + -0.7
Or see this region's nucleotide sequence