Experiment: KLEB29 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS09065 and BWI76_RS09070 are separated by 209 nucleotides BWI76_RS09070 and BWI76_RS09075 are separated by 4 nucleotides
BWI76_RS09065: BWI76_RS09065 - sugar phosphatase SupH, at 1,912,581 to 1,913,396
_RS09065
BWI76_RS09070: BWI76_RS09070 - formate C-acetyltransferase/glycerol dehydratase family glycyl radical enzyme, at 1,913,606 to 1,916,038
_RS09070
BWI76_RS09075: BWI76_RS09075 - pyruvate formate lyase-activating protein, at 1,916,043 to 1,916,942
_RS09075
Position (kb)
1913
1914
1915
1916
1917 Strain fitness (log2 ratio)
-2
-1
0
1 at 1912.791 kb on - strand, within BWI76_RS09065 at 1912.817 kb on - strand, within BWI76_RS09065 at 1912.827 kb on - strand, within BWI76_RS09065 at 1913.000 kb on + strand, within BWI76_RS09065 at 1913.593 kb on - strand at 1913.657 kb on - strand at 1913.713 kb on + strand at 1914.238 kb on - strand, within BWI76_RS09070 at 1914.290 kb on - strand, within BWI76_RS09070 at 1914.308 kb on - strand, within BWI76_RS09070 at 1914.388 kb on + strand, within BWI76_RS09070 at 1914.388 kb on + strand, within BWI76_RS09070 at 1914.396 kb on - strand, within BWI76_RS09070 at 1914.396 kb on - strand, within BWI76_RS09070 at 1914.528 kb on - strand, within BWI76_RS09070 at 1914.659 kb on + strand, within BWI76_RS09070 at 1914.868 kb on - strand, within BWI76_RS09070 at 1914.890 kb on + strand, within BWI76_RS09070 at 1915.140 kb on - strand, within BWI76_RS09070 at 1915.163 kb on - strand, within BWI76_RS09070 at 1915.382 kb on - strand, within BWI76_RS09070 at 1915.419 kb on + strand, within BWI76_RS09070 at 1915.666 kb on + strand, within BWI76_RS09070 at 1915.929 kb on + strand at 1915.945 kb on + strand at 1915.950 kb on + strand at 1915.950 kb on + strand at 1915.958 kb on - strand at 1916.068 kb on + strand at 1916.103 kb on - strand at 1916.122 kb on + strand at 1916.137 kb on + strand, within BWI76_RS09075 at 1916.137 kb on + strand, within BWI76_RS09075 at 1916.137 kb on + strand, within BWI76_RS09075 at 1916.137 kb on + strand, within BWI76_RS09075 at 1916.137 kb on + strand, within BWI76_RS09075 at 1916.137 kb on + strand, within BWI76_RS09075 at 1916.145 kb on - strand, within BWI76_RS09075 at 1916.145 kb on - strand, within BWI76_RS09075 at 1916.145 kb on - strand, within BWI76_RS09075 at 1916.209 kb on + strand, within BWI76_RS09075 at 1916.218 kb on - strand, within BWI76_RS09075 at 1916.337 kb on - strand, within BWI76_RS09075 at 1916.713 kb on - strand, within BWI76_RS09075
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB29 MOI 1 remove 1,912,791 - BWI76_RS09065 0.26 +0.2 1,912,817 - BWI76_RS09065 0.29 +0.2 1,912,827 - BWI76_RS09065 0.30 -1.3 1,913,000 + BWI76_RS09065 0.51 +0.4 1,913,593 - -1.2 1,913,657 - +0.7 1,913,713 + -1.6 1,914,238 - BWI76_RS09070 0.26 -2.1 1,914,290 - BWI76_RS09070 0.28 -0.3 1,914,308 - BWI76_RS09070 0.29 -0.9 1,914,388 + BWI76_RS09070 0.32 -1.2 1,914,388 + BWI76_RS09070 0.32 -2.3 1,914,396 - BWI76_RS09070 0.32 -0.4 1,914,396 - BWI76_RS09070 0.32 -0.2 1,914,528 - BWI76_RS09070 0.38 -0.2 1,914,659 + BWI76_RS09070 0.43 +0.4 1,914,868 - BWI76_RS09070 0.52 -0.8 1,914,890 + BWI76_RS09070 0.53 -0.4 1,915,140 - BWI76_RS09070 0.63 -1.3 1,915,163 - BWI76_RS09070 0.64 -1.6 1,915,382 - BWI76_RS09070 0.73 +0.3 1,915,419 + BWI76_RS09070 0.75 -0.6 1,915,666 + BWI76_RS09070 0.85 -0.2 1,915,929 + -1.2 1,915,945 + -2.6 1,915,950 + -0.3 1,915,950 + -0.3 1,915,958 - +0.6 1,916,068 + +1.6 1,916,103 - +0.0 1,916,122 + +1.2 1,916,137 + BWI76_RS09075 0.10 -1.7 1,916,137 + BWI76_RS09075 0.10 +0.6 1,916,137 + BWI76_RS09075 0.10 -0.2 1,916,137 + BWI76_RS09075 0.10 -0.7 1,916,137 + BWI76_RS09075 0.10 +0.5 1,916,137 + BWI76_RS09075 0.10 +1.6 1,916,145 - BWI76_RS09075 0.11 -1.0 1,916,145 - BWI76_RS09075 0.11 -1.2 1,916,145 - BWI76_RS09075 0.11 +0.3 1,916,209 + BWI76_RS09075 0.18 -0.4 1,916,218 - BWI76_RS09075 0.19 +0.2 1,916,337 - BWI76_RS09075 0.33 -0.4 1,916,713 - BWI76_RS09075 0.74 -0.3
Or see this region's nucleotide sequence