Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS06965

Experiment: KLEB29 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS06955 and BWI76_RS06960 are separated by 70 nucleotidesBWI76_RS06960 and BWI76_RS06965 are separated by 209 nucleotidesBWI76_RS06965 and BWI76_RS06970 are separated by 106 nucleotides BWI76_RS06955: BWI76_RS06955 - sugar ABC transporter permease, at 1,459,633 to 1,462,050 _RS06955 BWI76_RS06960: BWI76_RS06960 - monooxygenase, at 1,462,121 to 1,462,426 _RS06960 BWI76_RS06965: BWI76_RS06965 - membrane protein, at 1,462,636 to 1,463,781 _RS06965 BWI76_RS06970: BWI76_RS06970 - selenophosphate-dependent tRNA 2-selenouridine synthase, at 1,463,888 to 1,464,973 _RS06970 Position (kb) 1462 1463 1464Strain fitness (log2 ratio) -1 0 1 2 3at 1461.869 kb on + strandat 1462.278 kb on + strand, within BWI76_RS06960at 1462.692 kb on - strandat 1462.801 kb on + strand, within BWI76_RS06965at 1462.891 kb on - strand, within BWI76_RS06965at 1462.938 kb on - strand, within BWI76_RS06965at 1462.991 kb on - strand, within BWI76_RS06965at 1463.012 kb on - strand, within BWI76_RS06965at 1463.012 kb on - strand, within BWI76_RS06965at 1463.061 kb on - strand, within BWI76_RS06965at 1463.422 kb on + strand, within BWI76_RS06965at 1463.685 kb on + strandat 1463.775 kb on + strandat 1463.810 kb on + strandat 1463.873 kb on - strandat 1463.889 kb on - strandat 1464.424 kb on + strand, within BWI76_RS06970at 1464.550 kb on - strand, within BWI76_RS06970at 1464.601 kb on + strand, within BWI76_RS06970at 1464.622 kb on - strand, within BWI76_RS06970at 1464.637 kb on - strand, within BWI76_RS06970

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB29 MOI 1
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1,461,869 + -0.6
1,462,278 + BWI76_RS06960 0.51 +2.8
1,462,692 - -0.4
1,462,801 + BWI76_RS06965 0.14 -1.4
1,462,891 - BWI76_RS06965 0.22 -0.2
1,462,938 - BWI76_RS06965 0.26 -1.1
1,462,991 - BWI76_RS06965 0.31 -1.6
1,463,012 - BWI76_RS06965 0.33 -1.6
1,463,012 - BWI76_RS06965 0.33 -0.5
1,463,061 - BWI76_RS06965 0.37 -1.3
1,463,422 + BWI76_RS06965 0.69 -0.7
1,463,685 + +0.2
1,463,775 + -0.3
1,463,810 + -0.7
1,463,873 - -1.1
1,463,889 - -0.8
1,464,424 + BWI76_RS06970 0.49 +0.0
1,464,550 - BWI76_RS06970 0.61 -1.0
1,464,601 + BWI76_RS06970 0.66 -0.3
1,464,622 - BWI76_RS06970 0.68 -1.6
1,464,637 - BWI76_RS06970 0.69 -0.5

Or see this region's nucleotide sequence