Experiment: KLEB29 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS06955 and BWI76_RS06960 are separated by 70 nucleotides BWI76_RS06960 and BWI76_RS06965 are separated by 209 nucleotides BWI76_RS06965 and BWI76_RS06970 are separated by 106 nucleotides
BWI76_RS06955: BWI76_RS06955 - sugar ABC transporter permease, at 1,459,633 to 1,462,050
_RS06955
BWI76_RS06960: BWI76_RS06960 - monooxygenase, at 1,462,121 to 1,462,426
_RS06960
BWI76_RS06965: BWI76_RS06965 - membrane protein, at 1,462,636 to 1,463,781
_RS06965
BWI76_RS06970: BWI76_RS06970 - selenophosphate-dependent tRNA 2-selenouridine synthase, at 1,463,888 to 1,464,973
_RS06970
Position (kb)
1462
1463
1464 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1461.869 kb on + strand at 1462.278 kb on + strand, within BWI76_RS06960 at 1462.692 kb on - strand at 1462.801 kb on + strand, within BWI76_RS06965 at 1462.891 kb on - strand, within BWI76_RS06965 at 1462.938 kb on - strand, within BWI76_RS06965 at 1462.991 kb on - strand, within BWI76_RS06965 at 1463.012 kb on - strand, within BWI76_RS06965 at 1463.012 kb on - strand, within BWI76_RS06965 at 1463.061 kb on - strand, within BWI76_RS06965 at 1463.422 kb on + strand, within BWI76_RS06965 at 1463.685 kb on + strand at 1463.775 kb on + strand at 1463.810 kb on + strand at 1463.873 kb on - strand at 1463.889 kb on - strand at 1464.424 kb on + strand, within BWI76_RS06970 at 1464.550 kb on - strand, within BWI76_RS06970 at 1464.601 kb on + strand, within BWI76_RS06970 at 1464.622 kb on - strand, within BWI76_RS06970 at 1464.637 kb on - strand, within BWI76_RS06970
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB29 MOI 1 remove 1,461,869 + -0.6 1,462,278 + BWI76_RS06960 0.51 +2.8 1,462,692 - -0.4 1,462,801 + BWI76_RS06965 0.14 -1.4 1,462,891 - BWI76_RS06965 0.22 -0.2 1,462,938 - BWI76_RS06965 0.26 -1.1 1,462,991 - BWI76_RS06965 0.31 -1.6 1,463,012 - BWI76_RS06965 0.33 -1.6 1,463,012 - BWI76_RS06965 0.33 -0.5 1,463,061 - BWI76_RS06965 0.37 -1.3 1,463,422 + BWI76_RS06965 0.69 -0.7 1,463,685 + +0.2 1,463,775 + -0.3 1,463,810 + -0.7 1,463,873 - -1.1 1,463,889 - -0.8 1,464,424 + BWI76_RS06970 0.49 +0.0 1,464,550 - BWI76_RS06970 0.61 -1.0 1,464,601 + BWI76_RS06970 0.66 -0.3 1,464,622 - BWI76_RS06970 0.68 -1.6 1,464,637 - BWI76_RS06970 0.69 -0.5
Or see this region's nucleotide sequence