Experiment: KLEB29 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05155 and BWI76_RS05160 are separated by 92 nucleotides BWI76_RS05160 and BWI76_RS05165 are separated by 180 nucleotides BWI76_RS05165 and BWI76_RS05170 overlap by 8 nucleotides BWI76_RS05170 and BWI76_RS05175 are separated by 84 nucleotides
BWI76_RS05155: BWI76_RS05155 - iron-sulfur cluster insertion protein ErpA, at 1,085,131 to 1,085,475
_RS05155
BWI76_RS05160: BWI76_RS05160 - hypothetical protein, at 1,085,568 to 1,086,191
_RS05160
BWI76_RS05165: BWI76_RS05165 - cobalamin-binding protein, at 1,086,372 to 1,087,172
_RS05165
BWI76_RS05170: BWI76_RS05170 - 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase, at 1,087,165 to 1,087,863
_RS05170
BWI76_RS05175: BWI76_RS05175 - dGTPase, at 1,087,948 to 1,089,462
_RS05175
Position (kb)
1086
1087
1088 Strain fitness (log2 ratio)
-2
-1
0
1 at 1085.778 kb on + strand, within BWI76_RS05160 at 1085.855 kb on + strand, within BWI76_RS05160 at 1085.884 kb on + strand, within BWI76_RS05160 at 1086.174 kb on - strand at 1086.197 kb on + strand at 1086.204 kb on + strand at 1086.306 kb on + strand at 1086.314 kb on - strand at 1086.399 kb on + strand at 1086.425 kb on - strand at 1086.445 kb on - strand at 1086.540 kb on + strand, within BWI76_RS05165 at 1086.566 kb on + strand, within BWI76_RS05165 at 1086.574 kb on - strand, within BWI76_RS05165 at 1086.666 kb on + strand, within BWI76_RS05165 at 1087.021 kb on + strand, within BWI76_RS05165 at 1087.094 kb on - strand at 1087.133 kb on + strand at 1088.154 kb on + strand, within BWI76_RS05175 at 1088.156 kb on + strand, within BWI76_RS05175 at 1088.172 kb on - strand, within BWI76_RS05175
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB29 MOI 1 remove 1,085,778 + BWI76_RS05160 0.34 +0.2 1,085,855 + BWI76_RS05160 0.46 -1.2 1,085,884 + BWI76_RS05160 0.51 +0.2 1,086,174 - -1.6 1,086,197 + +0.0 1,086,204 + -0.3 1,086,306 + +0.4 1,086,314 - +0.6 1,086,399 + +0.9 1,086,425 - +1.4 1,086,445 - -0.1 1,086,540 + BWI76_RS05165 0.21 -0.3 1,086,566 + BWI76_RS05165 0.24 -1.5 1,086,574 - BWI76_RS05165 0.25 -1.3 1,086,666 + BWI76_RS05165 0.37 -1.3 1,087,021 + BWI76_RS05165 0.81 -1.7 1,087,094 - -2.6 1,087,133 + +1.6 1,088,154 + BWI76_RS05175 0.14 -0.3 1,088,156 + BWI76_RS05175 0.14 -0.1 1,088,172 - BWI76_RS05175 0.15 -2.2
Or see this region's nucleotide sequence