Experiment: KLEB31 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS27610 and BWI76_RS27615 are separated by 95 nucleotides BWI76_RS27615 and BWI76_RS27620 are separated by 185 nucleotides BWI76_RS27620 and BWI76_RS27625 are separated by 112 nucleotides
BWI76_RS27610: BWI76_RS27610 - hydroxyacid dehydrogenase, at 5,731,133 to 5,731,647
_RS27610
BWI76_RS27615: BWI76_RS27615 - putative Shikimate kinase, at 5,731,743 to 5,732,348
_RS27615
BWI76_RS27620: BWI76_RS27620 - hypothetical protein, at 5,732,534 to 5,733,001
_RS27620
BWI76_RS27625: BWI76_RS27625 - NADPH:quinone reductase, at 5,733,114 to 5,734,121
_RS27625
Position (kb) 5732
5733
5734 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5731.576 kb on - strand, within BWI76_RS27610 at 5731.627 kb on - strand at 5731.627 kb on - strand at 5731.650 kb on - strand at 5731.710 kb on + strand at 5731.718 kb on - strand at 5731.718 kb on - strand at 5731.727 kb on - strand at 5731.727 kb on - strand at 5731.852 kb on + strand, within BWI76_RS27615 at 5731.952 kb on + strand, within BWI76_RS27615 at 5731.952 kb on + strand, within BWI76_RS27615 at 5731.952 kb on + strand, within BWI76_RS27615 at 5731.960 kb on - strand, within BWI76_RS27615 at 5731.960 kb on - strand, within BWI76_RS27615 at 5731.960 kb on - strand, within BWI76_RS27615 at 5731.960 kb on - strand, within BWI76_RS27615 at 5731.989 kb on - strand, within BWI76_RS27615 at 5732.019 kb on + strand, within BWI76_RS27615 at 5732.153 kb on + strand, within BWI76_RS27615 at 5732.178 kb on - strand, within BWI76_RS27615 at 5732.280 kb on - strand, within BWI76_RS27615 at 5732.294 kb on + strand at 5732.348 kb on + strand at 5732.356 kb on - strand at 5732.356 kb on - strand at 5732.356 kb on - strand at 5732.356 kb on - strand at 5732.374 kb on + strand at 5732.432 kb on + strand at 5732.440 kb on - strand at 5732.460 kb on - strand at 5732.492 kb on - strand at 5732.568 kb on + strand at 5732.576 kb on - strand at 5732.576 kb on - strand at 5732.587 kb on + strand, within BWI76_RS27620 at 5732.624 kb on - strand, within BWI76_RS27620 at 5732.631 kb on + strand, within BWI76_RS27620 at 5732.639 kb on - strand, within BWI76_RS27620 at 5732.639 kb on - strand, within BWI76_RS27620 at 5732.925 kb on - strand, within BWI76_RS27620 at 5732.956 kb on - strand at 5733.066 kb on + strand at 5733.069 kb on - strand at 5733.070 kb on - strand at 5733.112 kb on + strand at 5733.389 kb on + strand, within BWI76_RS27625 at 5733.469 kb on - strand, within BWI76_RS27625 at 5733.532 kb on + strand, within BWI76_RS27625 at 5733.830 kb on + strand, within BWI76_RS27625 at 5733.870 kb on - strand, within BWI76_RS27625 at 5733.905 kb on - strand, within BWI76_RS27625 at 5733.924 kb on + strand, within BWI76_RS27625
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB31 MOI 1 remove 5,731,576 - BWI76_RS27610 0.86 -0.2 5,731,627 - +0.2 5,731,627 - +0.3 5,731,650 - +0.5 5,731,710 + +0.2 5,731,718 - -0.3 5,731,718 - +1.0 5,731,727 - -0.0 5,731,727 - +0.3 5,731,852 + BWI76_RS27615 0.18 -0.3 5,731,952 + BWI76_RS27615 0.34 +0.8 5,731,952 + BWI76_RS27615 0.34 +1.5 5,731,952 + BWI76_RS27615 0.34 -0.2 5,731,960 - BWI76_RS27615 0.36 +0.1 5,731,960 - BWI76_RS27615 0.36 -0.3 5,731,960 - BWI76_RS27615 0.36 +1.5 5,731,960 - BWI76_RS27615 0.36 -0.4 5,731,989 - BWI76_RS27615 0.41 -0.9 5,732,019 + BWI76_RS27615 0.46 +0.0 5,732,153 + BWI76_RS27615 0.68 +1.2 5,732,178 - BWI76_RS27615 0.72 -0.4 5,732,280 - BWI76_RS27615 0.89 -0.2 5,732,294 + -0.5 5,732,348 + +1.1 5,732,356 - -1.3 5,732,356 - +1.0 5,732,356 - +0.0 5,732,356 - -1.2 5,732,374 + -0.9 5,732,432 + -0.6 5,732,440 - -0.3 5,732,460 - -2.6 5,732,492 - -0.2 5,732,568 + -1.9 5,732,576 - +2.6 5,732,576 - +1.7 5,732,587 + BWI76_RS27620 0.11 -1.6 5,732,624 - BWI76_RS27620 0.19 -0.8 5,732,631 + BWI76_RS27620 0.21 -1.1 5,732,639 - BWI76_RS27620 0.22 -1.2 5,732,639 - BWI76_RS27620 0.22 +0.0 5,732,925 - BWI76_RS27620 0.84 -3.2 5,732,956 - +0.0 5,733,066 + -0.7 5,733,069 - -0.8 5,733,070 - +0.1 5,733,112 + +0.5 5,733,389 + BWI76_RS27625 0.27 +0.5 5,733,469 - BWI76_RS27625 0.35 +0.0 5,733,532 + BWI76_RS27625 0.41 -0.9 5,733,830 + BWI76_RS27625 0.71 -0.5 5,733,870 - BWI76_RS27625 0.75 +0.2 5,733,905 - BWI76_RS27625 0.78 -2.0 5,733,924 + BWI76_RS27625 0.80 +0.5
Or see this region's nucleotide sequence