Experiment: KLEB31 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS27545 and BWI76_RS27550 are separated by 159 nucleotides BWI76_RS27550 and BWI76_RS27555 overlap by 20 nucleotides BWI76_RS27555 and BWI76_RS27560 are separated by 50 nucleotides BWI76_RS27560 and BWI76_RS27565 are separated by 80 nucleotides
BWI76_RS27545: BWI76_RS27545 - sugar isomerase, at 5,718,272 to 5,719,003
_RS27545
BWI76_RS27550: BWI76_RS27550 - hypothetical protein, at 5,719,163 to 5,719,837
_RS27550
BWI76_RS27555: BWI76_RS27555 - putative sugar kinase/transcriptional regulator, ATPase domain protein, at 5,719,818 to 5,720,750
_RS27555
BWI76_RS27560: BWI76_RS27560 - hypothetical protein, at 5,720,801 to 5,721,661
_RS27560
BWI76_RS27565: BWI76_RS27565 - sugar aldolase, at 5,721,742 to 5,722,593
_RS27565
Position (kb) 5719
5720
5721 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 5718.881 kb on - strand, within BWI76_RS27545 at 5718.881 kb on - strand, within BWI76_RS27545 at 5718.902 kb on - strand, within BWI76_RS27545 at 5718.927 kb on - strand, within BWI76_RS27545 at 5718.927 kb on - strand, within BWI76_RS27545 at 5718.927 kb on - strand, within BWI76_RS27545 at 5718.927 kb on - strand, within BWI76_RS27545 at 5719.020 kb on - strand at 5719.150 kb on + strand at 5719.150 kb on + strand at 5719.151 kb on + strand at 5719.169 kb on + strand at 5719.176 kb on - strand at 5719.177 kb on - strand at 5719.177 kb on - strand at 5719.466 kb on - strand, within BWI76_RS27550 at 5719.481 kb on - strand, within BWI76_RS27550 at 5719.580 kb on + strand, within BWI76_RS27550 at 5719.580 kb on + strand, within BWI76_RS27550 at 5719.590 kb on - strand, within BWI76_RS27550 at 5719.590 kb on - strand, within BWI76_RS27550 at 5719.789 kb on - strand at 5719.796 kb on - strand at 5719.992 kb on - strand, within BWI76_RS27555 at 5720.129 kb on + strand, within BWI76_RS27555 at 5720.144 kb on + strand, within BWI76_RS27555 at 5720.152 kb on - strand, within BWI76_RS27555 at 5720.164 kb on - strand, within BWI76_RS27555 at 5720.240 kb on + strand, within BWI76_RS27555 at 5720.245 kb on + strand at 5720.245 kb on + strand, within BWI76_RS27555 at 5720.253 kb on - strand, within BWI76_RS27555 at 5720.253 kb on - strand, within BWI76_RS27555 at 5720.260 kb on + strand, within BWI76_RS27555 at 5720.278 kb on + strand, within BWI76_RS27555 at 5720.335 kb on + strand, within BWI76_RS27555 at 5720.511 kb on - strand, within BWI76_RS27555 at 5720.548 kb on - strand, within BWI76_RS27555 at 5720.548 kb on - strand, within BWI76_RS27555 at 5720.607 kb on + strand, within BWI76_RS27555 at 5720.613 kb on + strand, within BWI76_RS27555 at 5720.700 kb on + strand at 5720.755 kb on + strand at 5720.803 kb on + strand at 5720.804 kb on + strand at 5720.807 kb on - strand at 5720.811 kb on - strand at 5720.899 kb on + strand, within BWI76_RS27560 at 5721.270 kb on + strand, within BWI76_RS27560 at 5721.325 kb on - strand, within BWI76_RS27560 at 5721.371 kb on + strand, within BWI76_RS27560 at 5721.371 kb on + strand, within BWI76_RS27560 at 5721.388 kb on - strand, within BWI76_RS27560 at 5721.393 kb on - strand, within BWI76_RS27560 at 5721.447 kb on + strand, within BWI76_RS27560 at 5721.457 kb on + strand, within BWI76_RS27560 at 5721.579 kb on - strand at 5721.579 kb on - strand at 5721.693 kb on - strand at 5721.708 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB31 MOI 1 remove 5,718,881 - BWI76_RS27545 0.83 +0.9 5,718,881 - BWI76_RS27545 0.83 -0.6 5,718,902 - BWI76_RS27545 0.86 +3.0 5,718,927 - BWI76_RS27545 0.89 +2.6 5,718,927 - BWI76_RS27545 0.89 +1.6 5,718,927 - BWI76_RS27545 0.89 -0.6 5,718,927 - BWI76_RS27545 0.89 +0.8 5,719,020 - -4.2 5,719,150 + -1.6 5,719,150 + +0.6 5,719,151 + -0.5 5,719,169 + -0.3 5,719,176 - -0.3 5,719,177 - +0.4 5,719,177 - +1.7 5,719,466 - BWI76_RS27550 0.45 -0.4 5,719,481 - BWI76_RS27550 0.47 +1.9 5,719,580 + BWI76_RS27550 0.62 -1.4 5,719,580 + BWI76_RS27550 0.62 -3.3 5,719,590 - BWI76_RS27550 0.63 -0.1 5,719,590 - BWI76_RS27550 0.63 -1.6 5,719,789 - +0.5 5,719,796 - -1.0 5,719,992 - BWI76_RS27555 0.19 -1.6 5,720,129 + BWI76_RS27555 0.33 -0.7 5,720,144 + BWI76_RS27555 0.35 +0.4 5,720,152 - BWI76_RS27555 0.36 -0.6 5,720,164 - BWI76_RS27555 0.37 +2.3 5,720,240 + BWI76_RS27555 0.45 -0.5 5,720,245 + -1.3 5,720,245 + BWI76_RS27555 0.46 +1.2 5,720,253 - BWI76_RS27555 0.47 -1.9 5,720,253 - BWI76_RS27555 0.47 +1.6 5,720,260 + BWI76_RS27555 0.47 +1.8 5,720,278 + BWI76_RS27555 0.49 -0.6 5,720,335 + BWI76_RS27555 0.55 +1.6 5,720,511 - BWI76_RS27555 0.74 +1.0 5,720,548 - BWI76_RS27555 0.78 -0.6 5,720,548 - BWI76_RS27555 0.78 +1.0 5,720,607 + BWI76_RS27555 0.85 +0.2 5,720,613 + BWI76_RS27555 0.85 +1.2 5,720,700 + -1.8 5,720,755 + -0.6 5,720,803 + -2.6 5,720,804 + -1.0 5,720,807 - -0.1 5,720,811 - -0.4 5,720,899 + BWI76_RS27560 0.11 +1.5 5,721,270 + BWI76_RS27560 0.54 -0.1 5,721,325 - BWI76_RS27560 0.61 -0.7 5,721,371 + BWI76_RS27560 0.66 -0.4 5,721,371 + BWI76_RS27560 0.66 -1.6 5,721,388 - BWI76_RS27560 0.68 -0.4 5,721,393 - BWI76_RS27560 0.69 -0.5 5,721,447 + BWI76_RS27560 0.75 +2.4 5,721,457 + BWI76_RS27560 0.76 -0.8 5,721,579 - +0.2 5,721,579 - -1.4 5,721,693 - +0.0 5,721,708 - -1.7
Or see this region's nucleotide sequence