Experiment: KLEB31 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS27540 and BWI76_RS27545 are separated by 456 nucleotides BWI76_RS27545 and BWI76_RS27550 are separated by 159 nucleotides BWI76_RS27550 and BWI76_RS27555 overlap by 20 nucleotides
BWI76_RS27540: BWI76_RS27540 - glycoside hydrolase, at 5,716,436 to 5,717,815
_RS27540
BWI76_RS27545: BWI76_RS27545 - sugar isomerase, at 5,718,272 to 5,719,003
_RS27545
BWI76_RS27550: BWI76_RS27550 - hypothetical protein, at 5,719,163 to 5,719,837
_RS27550
BWI76_RS27555: BWI76_RS27555 - putative sugar kinase/transcriptional regulator, ATPase domain protein, at 5,719,818 to 5,720,750
_RS27555
Position (kb) 5718
5719
5720 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 5717.315 kb on + strand, within BWI76_RS27540 at 5717.316 kb on + strand, within BWI76_RS27540 at 5717.405 kb on + strand, within BWI76_RS27540 at 5717.412 kb on + strand, within BWI76_RS27540 at 5717.420 kb on - strand, within BWI76_RS27540 at 5717.422 kb on + strand, within BWI76_RS27540 at 5717.472 kb on + strand, within BWI76_RS27540 at 5717.535 kb on - strand, within BWI76_RS27540 at 5717.574 kb on + strand, within BWI76_RS27540 at 5717.584 kb on + strand, within BWI76_RS27540 at 5717.592 kb on - strand, within BWI76_RS27540 at 5717.620 kb on + strand, within BWI76_RS27540 at 5717.652 kb on + strand, within BWI76_RS27540 at 5717.908 kb on + strand at 5717.956 kb on + strand at 5717.964 kb on + strand at 5717.972 kb on - strand at 5717.974 kb on + strand at 5718.007 kb on + strand at 5718.015 kb on - strand at 5718.044 kb on + strand at 5718.096 kb on - strand at 5718.096 kb on - strand at 5718.469 kb on - strand, within BWI76_RS27545 at 5718.508 kb on - strand, within BWI76_RS27545 at 5718.561 kb on + strand, within BWI76_RS27545 at 5718.571 kb on - strand, within BWI76_RS27545 at 5718.774 kb on + strand, within BWI76_RS27545 at 5718.775 kb on + strand, within BWI76_RS27545 at 5718.881 kb on - strand, within BWI76_RS27545 at 5718.881 kb on - strand, within BWI76_RS27545 at 5718.902 kb on - strand, within BWI76_RS27545 at 5718.927 kb on - strand, within BWI76_RS27545 at 5718.927 kb on - strand, within BWI76_RS27545 at 5718.927 kb on - strand, within BWI76_RS27545 at 5718.927 kb on - strand, within BWI76_RS27545 at 5719.020 kb on - strand at 5719.150 kb on + strand at 5719.150 kb on + strand at 5719.151 kb on + strand at 5719.169 kb on + strand at 5719.176 kb on - strand at 5719.177 kb on - strand at 5719.177 kb on - strand at 5719.466 kb on - strand, within BWI76_RS27550 at 5719.481 kb on - strand, within BWI76_RS27550 at 5719.580 kb on + strand, within BWI76_RS27550 at 5719.580 kb on + strand, within BWI76_RS27550 at 5719.590 kb on - strand, within BWI76_RS27550 at 5719.590 kb on - strand, within BWI76_RS27550 at 5719.789 kb on - strand at 5719.796 kb on - strand at 5719.992 kb on - strand, within BWI76_RS27555
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB31 MOI 1 remove 5,717,315 + BWI76_RS27540 0.64 +0.1 5,717,316 + BWI76_RS27540 0.64 -1.2 5,717,405 + BWI76_RS27540 0.70 -0.6 5,717,412 + BWI76_RS27540 0.71 +4.1 5,717,420 - BWI76_RS27540 0.71 +0.1 5,717,422 + BWI76_RS27540 0.71 +0.7 5,717,472 + BWI76_RS27540 0.75 -0.1 5,717,535 - BWI76_RS27540 0.80 -0.2 5,717,574 + BWI76_RS27540 0.82 +0.3 5,717,584 + BWI76_RS27540 0.83 +0.9 5,717,592 - BWI76_RS27540 0.84 +0.1 5,717,620 + BWI76_RS27540 0.86 -0.5 5,717,652 + BWI76_RS27540 0.88 +0.3 5,717,908 + +0.5 5,717,956 + +2.6 5,717,964 + +1.8 5,717,972 - +0.3 5,717,974 + +3.0 5,718,007 + +0.3 5,718,015 - -1.5 5,718,044 + +0.5 5,718,096 - +0.2 5,718,096 - +0.5 5,718,469 - BWI76_RS27545 0.27 -1.1 5,718,508 - BWI76_RS27545 0.32 +0.2 5,718,561 + BWI76_RS27545 0.39 +0.5 5,718,571 - BWI76_RS27545 0.41 -0.0 5,718,774 + BWI76_RS27545 0.69 +1.9 5,718,775 + BWI76_RS27545 0.69 +0.5 5,718,881 - BWI76_RS27545 0.83 +0.9 5,718,881 - BWI76_RS27545 0.83 -0.6 5,718,902 - BWI76_RS27545 0.86 +3.0 5,718,927 - BWI76_RS27545 0.89 +2.6 5,718,927 - BWI76_RS27545 0.89 +1.6 5,718,927 - BWI76_RS27545 0.89 -0.6 5,718,927 - BWI76_RS27545 0.89 +0.8 5,719,020 - -4.2 5,719,150 + -1.6 5,719,150 + +0.6 5,719,151 + -0.5 5,719,169 + -0.3 5,719,176 - -0.3 5,719,177 - +0.4 5,719,177 - +1.7 5,719,466 - BWI76_RS27550 0.45 -0.4 5,719,481 - BWI76_RS27550 0.47 +1.9 5,719,580 + BWI76_RS27550 0.62 -1.4 5,719,580 + BWI76_RS27550 0.62 -3.3 5,719,590 - BWI76_RS27550 0.63 -0.1 5,719,590 - BWI76_RS27550 0.63 -1.6 5,719,789 - +0.5 5,719,796 - -1.0 5,719,992 - BWI76_RS27555 0.19 -1.6
Or see this region's nucleotide sequence