Experiment: KLEB31 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS26615 and BWI76_RS26620 are separated by 331 nucleotides BWI76_RS26620 and BWI76_RS26625 are separated by 58 nucleotides BWI76_RS26625 and BWI76_RS26630 are separated by 37 nucleotides BWI76_RS26630 and BWI76_RS26635 are separated by 111 nucleotides
BWI76_RS26615: BWI76_RS26615 - gluconate:H+ symporter (from data) , at 5,510,053 to 5,511,369
_RS26615
BWI76_RS26620: BWI76_RS26620 - Fe-S biogenesis protein NfuA, at 5,511,701 to 5,512,276
_RS26620
BWI76_RS26625: BWI76_RS26625 - gluconate transporter periplasmic gluconate-binding protein, at 5,512,335 to 5,513,009
_RS26625
BWI76_RS26630: BWI76_RS26630 - pimeloyl-[acyl-carrier protein] methyl ester esterase, at 5,513,047 to 5,513,829
_RS26630
BWI76_RS26635: BWI76_RS26635 - hypothetical protein, at 5,513,941 to 5,514,210
_RS26635
Position (kb)
5512
5513
5514 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 5511.370 kb on + strand at 5511.658 kb on - strand at 5511.659 kb on + strand at 5511.664 kb on - strand at 5512.114 kb on - strand, within BWI76_RS26620 at 5512.362 kb on - strand at 5512.511 kb on + strand, within BWI76_RS26625 at 5512.734 kb on + strand, within BWI76_RS26625 at 5512.743 kb on - strand, within BWI76_RS26625 at 5512.770 kb on - strand, within BWI76_RS26625 at 5512.860 kb on + strand, within BWI76_RS26625 at 5513.030 kb on + strand at 5513.108 kb on + strand at 5513.138 kb on + strand, within BWI76_RS26630 at 5513.141 kb on - strand, within BWI76_RS26630 at 5513.324 kb on - strand, within BWI76_RS26630 at 5513.375 kb on + strand, within BWI76_RS26630 at 5513.524 kb on + strand, within BWI76_RS26630 at 5513.669 kb on + strand, within BWI76_RS26630 at 5513.797 kb on + strand at 5513.930 kb on + strand at 5513.984 kb on + strand, within BWI76_RS26635 at 5513.988 kb on + strand, within BWI76_RS26635 at 5513.996 kb on - strand, within BWI76_RS26635
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB31 MOI 1 remove 5,511,370 + -1.0 5,511,658 - -1.8 5,511,659 + -0.1 5,511,664 - +0.3 5,512,114 - BWI76_RS26620 0.72 +1.4 5,512,362 - -0.7 5,512,511 + BWI76_RS26625 0.26 -0.1 5,512,734 + BWI76_RS26625 0.59 -1.1 5,512,743 - BWI76_RS26625 0.60 -1.0 5,512,770 - BWI76_RS26625 0.64 -1.0 5,512,860 + BWI76_RS26625 0.78 -0.9 5,513,030 + -0.5 5,513,108 + +0.6 5,513,138 + BWI76_RS26630 0.12 -0.3 5,513,141 - BWI76_RS26630 0.12 +0.0 5,513,324 - BWI76_RS26630 0.35 +3.8 5,513,375 + BWI76_RS26630 0.42 -2.1 5,513,524 + BWI76_RS26630 0.61 +2.0 5,513,669 + BWI76_RS26630 0.79 +1.8 5,513,797 + +0.6 5,513,930 + +0.3 5,513,984 + BWI76_RS26635 0.16 -0.7 5,513,988 + BWI76_RS26635 0.17 +1.4 5,513,996 - BWI76_RS26635 0.20 +0.5
Or see this region's nucleotide sequence