Experiment: KLEB31 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS26055 and BWI76_RS26060 are separated by 89 nucleotides BWI76_RS26060 and BWI76_RS26065 are separated by 3 nucleotides BWI76_RS26065 and BWI76_RS26070 overlap by 8 nucleotides
BWI76_RS26055: BWI76_RS26055 - siroheme synthase, at 5,383,966 to 5,385,339
_RS26055
BWI76_RS26060: BWI76_RS26060 - tryptophan--tRNA ligase, at 5,385,429 to 5,386,433
_RS26060
BWI76_RS26065: BWI76_RS26065 - phosphoglycolate phosphatase, at 5,386,437 to 5,387,186
_RS26065
BWI76_RS26070: BWI76_RS26070 - ribulose-phosphate 3-epimerase, at 5,387,179 to 5,387,856
_RS26070
Position (kb)
5385
5386
5387 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 5384.441 kb on - strand, within BWI76_RS26055 at 5384.653 kb on - strand, within BWI76_RS26055 at 5384.661 kb on - strand, within BWI76_RS26055 at 5384.737 kb on - strand, within BWI76_RS26055 at 5384.782 kb on + strand, within BWI76_RS26055 at 5384.790 kb on - strand, within BWI76_RS26055 at 5384.805 kb on - strand, within BWI76_RS26055 at 5384.904 kb on + strand, within BWI76_RS26055 at 5385.042 kb on + strand, within BWI76_RS26055 at 5385.042 kb on + strand, within BWI76_RS26055 at 5385.042 kb on + strand, within BWI76_RS26055 at 5385.050 kb on - strand, within BWI76_RS26055 at 5385.195 kb on - strand, within BWI76_RS26055 at 5385.216 kb on - strand at 5385.230 kb on + strand at 5385.238 kb on - strand at 5385.238 kb on - strand at 5385.304 kb on - strand at 5385.353 kb on + strand at 5385.427 kb on + strand at 5385.428 kb on - strand at 5385.440 kb on - strand at 5385.542 kb on - strand, within BWI76_RS26060 at 5385.542 kb on - strand, within BWI76_RS26060 at 5385.564 kb on + strand, within BWI76_RS26060 at 5385.610 kb on + strand, within BWI76_RS26060 at 5385.706 kb on - strand, within BWI76_RS26060 at 5385.867 kb on - strand, within BWI76_RS26060 at 5386.074 kb on - strand, within BWI76_RS26060 at 5386.074 kb on - strand, within BWI76_RS26060 at 5386.074 kb on - strand, within BWI76_RS26060 at 5386.190 kb on + strand, within BWI76_RS26060 at 5386.236 kb on - strand, within BWI76_RS26060 at 5386.258 kb on + strand, within BWI76_RS26060 at 5386.266 kb on - strand, within BWI76_RS26060 at 5386.266 kb on - strand, within BWI76_RS26060 at 5386.393 kb on + strand at 5386.393 kb on + strand at 5386.401 kb on - strand at 5386.542 kb on - strand, within BWI76_RS26065 at 5386.657 kb on + strand, within BWI76_RS26065 at 5386.746 kb on + strand, within BWI76_RS26065 at 5386.993 kb on + strand, within BWI76_RS26065 at 5387.143 kb on - strand at 5387.194 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB31 MOI 1 remove 5,384,441 - BWI76_RS26055 0.35 -1.2 5,384,653 - BWI76_RS26055 0.50 +0.4 5,384,661 - BWI76_RS26055 0.51 +1.3 5,384,737 - BWI76_RS26055 0.56 -1.8 5,384,782 + BWI76_RS26055 0.59 +0.0 5,384,790 - BWI76_RS26055 0.60 -1.6 5,384,805 - BWI76_RS26055 0.61 +0.9 5,384,904 + BWI76_RS26055 0.68 -0.7 5,385,042 + BWI76_RS26055 0.78 +1.8 5,385,042 + BWI76_RS26055 0.78 -0.3 5,385,042 + BWI76_RS26055 0.78 +0.8 5,385,050 - BWI76_RS26055 0.79 +0.0 5,385,195 - BWI76_RS26055 0.89 +1.8 5,385,216 - -0.8 5,385,230 + -0.7 5,385,238 - +0.9 5,385,238 - -0.2 5,385,304 - -0.0 5,385,353 + +0.0 5,385,427 + -0.3 5,385,428 - +0.1 5,385,440 - +0.2 5,385,542 - BWI76_RS26060 0.11 +0.6 5,385,542 - BWI76_RS26060 0.11 +0.9 5,385,564 + BWI76_RS26060 0.13 +2.4 5,385,610 + BWI76_RS26060 0.18 +2.9 5,385,706 - BWI76_RS26060 0.28 +0.4 5,385,867 - BWI76_RS26060 0.44 -1.1 5,386,074 - BWI76_RS26060 0.64 +1.9 5,386,074 - BWI76_RS26060 0.64 +0.9 5,386,074 - BWI76_RS26060 0.64 +0.6 5,386,190 + BWI76_RS26060 0.76 -0.7 5,386,236 - BWI76_RS26060 0.80 -1.0 5,386,258 + BWI76_RS26060 0.82 +1.6 5,386,266 - BWI76_RS26060 0.83 +0.0 5,386,266 - BWI76_RS26060 0.83 +0.2 5,386,393 + +2.1 5,386,393 + +0.3 5,386,401 - -2.1 5,386,542 - BWI76_RS26065 0.14 -2.7 5,386,657 + BWI76_RS26065 0.29 +1.6 5,386,746 + BWI76_RS26065 0.41 -0.2 5,386,993 + BWI76_RS26065 0.74 +2.3 5,387,143 - -1.6 5,387,194 - -0.2
Or see this region's nucleotide sequence