Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS26060

Experiment: KLEB31 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS26055 and BWI76_RS26060 are separated by 89 nucleotidesBWI76_RS26060 and BWI76_RS26065 are separated by 3 nucleotidesBWI76_RS26065 and BWI76_RS26070 overlap by 8 nucleotides BWI76_RS26055: BWI76_RS26055 - siroheme synthase, at 5,383,966 to 5,385,339 _RS26055 BWI76_RS26060: BWI76_RS26060 - tryptophan--tRNA ligase, at 5,385,429 to 5,386,433 _RS26060 BWI76_RS26065: BWI76_RS26065 - phosphoglycolate phosphatase, at 5,386,437 to 5,387,186 _RS26065 BWI76_RS26070: BWI76_RS26070 - ribulose-phosphate 3-epimerase, at 5,387,179 to 5,387,856 _RS26070 Position (kb) 5385 5386 5387Strain fitness (log2 ratio) -2 -1 0 1 2 3at 5384.441 kb on - strand, within BWI76_RS26055at 5384.653 kb on - strand, within BWI76_RS26055at 5384.661 kb on - strand, within BWI76_RS26055at 5384.737 kb on - strand, within BWI76_RS26055at 5384.782 kb on + strand, within BWI76_RS26055at 5384.790 kb on - strand, within BWI76_RS26055at 5384.805 kb on - strand, within BWI76_RS26055at 5384.904 kb on + strand, within BWI76_RS26055at 5385.042 kb on + strand, within BWI76_RS26055at 5385.042 kb on + strand, within BWI76_RS26055at 5385.042 kb on + strand, within BWI76_RS26055at 5385.050 kb on - strand, within BWI76_RS26055at 5385.195 kb on - strand, within BWI76_RS26055at 5385.216 kb on - strandat 5385.230 kb on + strandat 5385.238 kb on - strandat 5385.238 kb on - strandat 5385.304 kb on - strandat 5385.353 kb on + strandat 5385.427 kb on + strandat 5385.428 kb on - strandat 5385.440 kb on - strandat 5385.542 kb on - strand, within BWI76_RS26060at 5385.542 kb on - strand, within BWI76_RS26060at 5385.564 kb on + strand, within BWI76_RS26060at 5385.610 kb on + strand, within BWI76_RS26060at 5385.706 kb on - strand, within BWI76_RS26060at 5385.867 kb on - strand, within BWI76_RS26060at 5386.074 kb on - strand, within BWI76_RS26060at 5386.074 kb on - strand, within BWI76_RS26060at 5386.074 kb on - strand, within BWI76_RS26060at 5386.190 kb on + strand, within BWI76_RS26060at 5386.236 kb on - strand, within BWI76_RS26060at 5386.258 kb on + strand, within BWI76_RS26060at 5386.266 kb on - strand, within BWI76_RS26060at 5386.266 kb on - strand, within BWI76_RS26060at 5386.393 kb on + strandat 5386.393 kb on + strandat 5386.401 kb on - strandat 5386.542 kb on - strand, within BWI76_RS26065at 5386.657 kb on + strand, within BWI76_RS26065at 5386.746 kb on + strand, within BWI76_RS26065at 5386.993 kb on + strand, within BWI76_RS26065at 5387.143 kb on - strandat 5387.194 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB31 MOI 1
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5,384,441 - BWI76_RS26055 0.35 -1.2
5,384,653 - BWI76_RS26055 0.50 +0.4
5,384,661 - BWI76_RS26055 0.51 +1.3
5,384,737 - BWI76_RS26055 0.56 -1.8
5,384,782 + BWI76_RS26055 0.59 +0.0
5,384,790 - BWI76_RS26055 0.60 -1.6
5,384,805 - BWI76_RS26055 0.61 +0.9
5,384,904 + BWI76_RS26055 0.68 -0.7
5,385,042 + BWI76_RS26055 0.78 +1.8
5,385,042 + BWI76_RS26055 0.78 -0.3
5,385,042 + BWI76_RS26055 0.78 +0.8
5,385,050 - BWI76_RS26055 0.79 +0.0
5,385,195 - BWI76_RS26055 0.89 +1.8
5,385,216 - -0.8
5,385,230 + -0.7
5,385,238 - +0.9
5,385,238 - -0.2
5,385,304 - -0.0
5,385,353 + +0.0
5,385,427 + -0.3
5,385,428 - +0.1
5,385,440 - +0.2
5,385,542 - BWI76_RS26060 0.11 +0.6
5,385,542 - BWI76_RS26060 0.11 +0.9
5,385,564 + BWI76_RS26060 0.13 +2.4
5,385,610 + BWI76_RS26060 0.18 +2.9
5,385,706 - BWI76_RS26060 0.28 +0.4
5,385,867 - BWI76_RS26060 0.44 -1.1
5,386,074 - BWI76_RS26060 0.64 +1.9
5,386,074 - BWI76_RS26060 0.64 +0.9
5,386,074 - BWI76_RS26060 0.64 +0.6
5,386,190 + BWI76_RS26060 0.76 -0.7
5,386,236 - BWI76_RS26060 0.80 -1.0
5,386,258 + BWI76_RS26060 0.82 +1.6
5,386,266 - BWI76_RS26060 0.83 +0.0
5,386,266 - BWI76_RS26060 0.83 +0.2
5,386,393 + +2.1
5,386,393 + +0.3
5,386,401 - -2.1
5,386,542 - BWI76_RS26065 0.14 -2.7
5,386,657 + BWI76_RS26065 0.29 +1.6
5,386,746 + BWI76_RS26065 0.41 -0.2
5,386,993 + BWI76_RS26065 0.74 +2.3
5,387,143 - -1.6
5,387,194 - -0.2

Or see this region's nucleotide sequence