Experiment: KLEB31 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS25955 and BWI76_RS25960 are separated by 196 nucleotides BWI76_RS25960 and BWI76_RS25965 are separated by 81 nucleotides BWI76_RS25965 and BWI76_RS25970 overlap by 4 nucleotides BWI76_RS25970 and BWI76_RS25975 are separated by 35 nucleotides
BWI76_RS25955: BWI76_RS25955 - ribose-phosphate pyrophosphokinase, at 5,366,330 to 5,367,199
_RS25955
BWI76_RS25960: BWI76_RS25960 - NUDIX hydrolase, at 5,367,396 to 5,368,115
_RS25960
BWI76_RS25965: BWI76_RS25965 - hydrolase, at 5,368,197 to 5,369,219
_RS25965
BWI76_RS25970: BWI76_RS25970 - hypothetical protein, at 5,369,216 to 5,369,434
_RS25970
BWI76_RS25975: BWI76_RS25975 - phosphoribulokinase, at 5,369,470 to 5,370,339
_RS25975
Position (kb) 5368
5369
5370 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 5367.392 kb on + strand at 5367.392 kb on + strand at 5367.485 kb on + strand at 5367.493 kb on - strand, within BWI76_RS25960 at 5367.603 kb on + strand, within BWI76_RS25960 at 5367.739 kb on + strand, within BWI76_RS25960 at 5367.800 kb on - strand, within BWI76_RS25960 at 5367.877 kb on + strand, within BWI76_RS25960 at 5367.900 kb on + strand, within BWI76_RS25960 at 5367.914 kb on - strand, within BWI76_RS25960 at 5367.963 kb on - strand, within BWI76_RS25960 at 5368.096 kb on - strand at 5368.122 kb on + strand at 5368.130 kb on - strand at 5368.197 kb on + strand at 5368.640 kb on - strand, within BWI76_RS25965 at 5368.806 kb on + strand, within BWI76_RS25965 at 5368.858 kb on + strand, within BWI76_RS25965 at 5368.937 kb on - strand, within BWI76_RS25965 at 5368.937 kb on - strand, within BWI76_RS25965 at 5369.035 kb on - strand, within BWI76_RS25965 at 5369.271 kb on - strand, within BWI76_RS25970 at 5369.314 kb on - strand, within BWI76_RS25970 at 5369.379 kb on + strand, within BWI76_RS25970 at 5369.392 kb on - strand, within BWI76_RS25970 at 5369.392 kb on - strand, within BWI76_RS25970 at 5369.503 kb on + strand at 5369.503 kb on + strand at 5369.711 kb on - strand, within BWI76_RS25975 at 5369.746 kb on - strand, within BWI76_RS25975 at 5369.868 kb on - strand, within BWI76_RS25975 at 5370.107 kb on + strand, within BWI76_RS25975 at 5370.115 kb on - strand, within BWI76_RS25975
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB31 MOI 1 remove 5,367,392 + +0.3 5,367,392 + +0.5 5,367,485 + +0.9 5,367,493 - BWI76_RS25960 0.13 -1.3 5,367,603 + BWI76_RS25960 0.29 -1.4 5,367,739 + BWI76_RS25960 0.48 -2.5 5,367,800 - BWI76_RS25960 0.56 +1.8 5,367,877 + BWI76_RS25960 0.67 -1.0 5,367,900 + BWI76_RS25960 0.70 +0.2 5,367,914 - BWI76_RS25960 0.72 +2.9 5,367,963 - BWI76_RS25960 0.79 +2.0 5,368,096 - +0.2 5,368,122 + -0.9 5,368,130 - -1.3 5,368,197 + +0.8 5,368,640 - BWI76_RS25965 0.43 -1.3 5,368,806 + BWI76_RS25965 0.60 +0.1 5,368,858 + BWI76_RS25965 0.65 +0.0 5,368,937 - BWI76_RS25965 0.72 +0.5 5,368,937 - BWI76_RS25965 0.72 +0.6 5,369,035 - BWI76_RS25965 0.82 -0.8 5,369,271 - BWI76_RS25970 0.25 +0.6 5,369,314 - BWI76_RS25970 0.45 -0.1 5,369,379 + BWI76_RS25970 0.74 +0.6 5,369,392 - BWI76_RS25970 0.80 -1.2 5,369,392 - BWI76_RS25970 0.80 +0.5 5,369,503 + +2.5 5,369,503 + -0.0 5,369,711 - BWI76_RS25975 0.28 -0.2 5,369,746 - BWI76_RS25975 0.32 +0.0 5,369,868 - BWI76_RS25975 0.46 +0.1 5,370,107 + BWI76_RS25975 0.73 +1.2 5,370,115 - BWI76_RS25975 0.74 +1.2
Or see this region's nucleotide sequence