Experiment: KLEB31 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS25950 and BWI76_RS25955 are separated by 4 nucleotides BWI76_RS25955 and BWI76_RS25960 are separated by 196 nucleotides BWI76_RS25960 and BWI76_RS25965 are separated by 81 nucleotides
BWI76_RS25950: BWI76_RS25950 - nicotinate phosphoribosyltransferase, at 5,364,835 to 5,366,325
_RS25950
BWI76_RS25955: BWI76_RS25955 - ribose-phosphate pyrophosphokinase, at 5,366,330 to 5,367,199
_RS25955
BWI76_RS25960: BWI76_RS25960 - NUDIX hydrolase, at 5,367,396 to 5,368,115
_RS25960
BWI76_RS25965: BWI76_RS25965 - hydrolase, at 5,368,197 to 5,369,219
_RS25965
Position (kb) 5366
5367
5368 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 5365.408 kb on + strand, within BWI76_RS25950 at 5365.416 kb on - strand, within BWI76_RS25950 at 5365.686 kb on + strand, within BWI76_RS25950 at 5365.724 kb on + strand, within BWI76_RS25950 at 5365.724 kb on + strand, within BWI76_RS25950 at 5365.727 kb on - strand, within BWI76_RS25950 at 5365.727 kb on - strand, within BWI76_RS25950 at 5365.732 kb on - strand, within BWI76_RS25950 at 5365.870 kb on - strand, within BWI76_RS25950 at 5365.934 kb on - strand, within BWI76_RS25950 at 5366.113 kb on + strand, within BWI76_RS25950 at 5366.136 kb on - strand, within BWI76_RS25950 at 5366.453 kb on + strand at 5366.499 kb on - strand, within BWI76_RS25955 at 5366.516 kb on + strand, within BWI76_RS25955 at 5366.521 kb on + strand, within BWI76_RS25955 at 5366.522 kb on + strand, within BWI76_RS25955 at 5366.564 kb on - strand, within BWI76_RS25955 at 5366.619 kb on - strand, within BWI76_RS25955 at 5366.624 kb on - strand, within BWI76_RS25955 at 5366.705 kb on - strand, within BWI76_RS25955 at 5366.760 kb on + strand, within BWI76_RS25955 at 5366.782 kb on + strand, within BWI76_RS25955 at 5366.782 kb on + strand, within BWI76_RS25955 at 5366.788 kb on - strand, within BWI76_RS25955 at 5366.790 kb on - strand, within BWI76_RS25955 at 5366.876 kb on + strand, within BWI76_RS25955 at 5367.144 kb on + strand at 5367.392 kb on + strand at 5367.392 kb on + strand at 5367.485 kb on + strand at 5367.493 kb on - strand, within BWI76_RS25960 at 5367.603 kb on + strand, within BWI76_RS25960 at 5367.739 kb on + strand, within BWI76_RS25960 at 5367.800 kb on - strand, within BWI76_RS25960 at 5367.877 kb on + strand, within BWI76_RS25960 at 5367.900 kb on + strand, within BWI76_RS25960 at 5367.914 kb on - strand, within BWI76_RS25960 at 5367.963 kb on - strand, within BWI76_RS25960 at 5368.096 kb on - strand at 5368.122 kb on + strand at 5368.130 kb on - strand at 5368.197 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB31 MOI 1 remove 5,365,408 + BWI76_RS25950 0.38 +0.9 5,365,416 - BWI76_RS25950 0.39 +0.0 5,365,686 + BWI76_RS25950 0.57 -1.7 5,365,724 + BWI76_RS25950 0.60 +1.4 5,365,724 + BWI76_RS25950 0.60 -2.7 5,365,727 - BWI76_RS25950 0.60 -0.5 5,365,727 - BWI76_RS25950 0.60 +2.2 5,365,732 - BWI76_RS25950 0.60 -1.5 5,365,870 - BWI76_RS25950 0.69 -0.9 5,365,934 - BWI76_RS25950 0.74 -0.0 5,366,113 + BWI76_RS25950 0.86 +1.1 5,366,136 - BWI76_RS25950 0.87 -1.0 5,366,453 + +3.1 5,366,499 - BWI76_RS25955 0.19 +2.0 5,366,516 + BWI76_RS25955 0.21 +0.6 5,366,521 + BWI76_RS25955 0.22 +2.0 5,366,522 + BWI76_RS25955 0.22 -1.6 5,366,564 - BWI76_RS25955 0.27 +0.4 5,366,619 - BWI76_RS25955 0.33 +4.2 5,366,624 - BWI76_RS25955 0.34 +0.2 5,366,705 - BWI76_RS25955 0.43 -3.4 5,366,760 + BWI76_RS25955 0.49 -1.4 5,366,782 + BWI76_RS25955 0.52 +2.0 5,366,782 + BWI76_RS25955 0.52 -1.0 5,366,788 - BWI76_RS25955 0.53 -1.0 5,366,790 - BWI76_RS25955 0.53 +1.7 5,366,876 + BWI76_RS25955 0.63 +0.9 5,367,144 + -0.1 5,367,392 + +0.3 5,367,392 + +0.5 5,367,485 + +0.9 5,367,493 - BWI76_RS25960 0.13 -1.3 5,367,603 + BWI76_RS25960 0.29 -1.4 5,367,739 + BWI76_RS25960 0.48 -2.5 5,367,800 - BWI76_RS25960 0.56 +1.8 5,367,877 + BWI76_RS25960 0.67 -1.0 5,367,900 + BWI76_RS25960 0.70 +0.2 5,367,914 - BWI76_RS25960 0.72 +2.9 5,367,963 - BWI76_RS25960 0.79 +2.0 5,368,096 - +0.2 5,368,122 + -0.9 5,368,130 - -1.3 5,368,197 + +0.8
Or see this region's nucleotide sequence