Experiment: KLEB31 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS25915 and BWI76_RS25920 are separated by 59 nucleotides BWI76_RS25920 and BWI76_RS25925 are separated by 94 nucleotides BWI76_RS25925 and BWI76_RS25930 are separated by 11 nucleotides BWI76_RS25930 and BWI76_RS25935 overlap by 14 nucleotides BWI76_RS25935 and BWI76_RS25940 overlap by 80 nucleotides BWI76_RS25940 and BWI76_RS25945 are separated by 21 nucleotides
BWI76_RS25915: BWI76_RS25915 - protein SlyX, at 5,359,169 to 5,359,387
_RS25915
BWI76_RS25920: BWI76_RS25920 - peptidylprolyl isomerase, at 5,359,447 to 5,360,034
_RS25920
BWI76_RS25925: BWI76_RS25925 - hypothetical protein, at 5,360,129 to 5,360,329
_RS25925
BWI76_RS25930: BWI76_RS25930 - glutathione-regulated potassium-efflux system protein KefB, at 5,360,341 to 5,362,146
_RS25930
BWI76_RS25935: BWI76_RS25935 - glutathione-regulated potassium-efflux system ancillary protein KefG, at 5,362,133 to 5,362,693
_RS25935
BWI76_RS25940: BWI76_RS25940 - hypothetical protein, at 5,362,614 to 5,362,847
_RS25940
BWI76_RS25945: BWI76_RS25945 - ABC transporter ATP-binding protein, at 5,362,869 to 5,364,773
_RS25945
Position (kb) 5360
5361
5362
5363 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 5359.521 kb on - strand, within BWI76_RS25920 at 5359.585 kb on + strand, within BWI76_RS25920 at 5359.835 kb on - strand, within BWI76_RS25920 at 5359.993 kb on - strand at 5360.185 kb on + strand, within BWI76_RS25925 at 5360.254 kb on - strand, within BWI76_RS25925 at 5360.278 kb on - strand, within BWI76_RS25925 at 5360.425 kb on - strand at 5360.450 kb on - strand at 5360.628 kb on + strand, within BWI76_RS25930 at 5360.703 kb on - strand, within BWI76_RS25930 at 5361.199 kb on + strand, within BWI76_RS25930 at 5361.259 kb on + strand, within BWI76_RS25930 at 5361.267 kb on - strand, within BWI76_RS25930 at 5361.389 kb on + strand, within BWI76_RS25930 at 5361.407 kb on - strand, within BWI76_RS25930 at 5361.478 kb on + strand, within BWI76_RS25930 at 5361.493 kb on + strand, within BWI76_RS25930 at 5361.542 kb on - strand, within BWI76_RS25930 at 5361.692 kb on + strand, within BWI76_RS25930 at 5361.706 kb on - strand, within BWI76_RS25930 at 5361.724 kb on + strand, within BWI76_RS25930 at 5361.741 kb on - strand, within BWI76_RS25930 at 5361.932 kb on - strand, within BWI76_RS25930 at 5362.003 kb on + strand at 5362.211 kb on + strand, within BWI76_RS25935 at 5362.438 kb on - strand, within BWI76_RS25935 at 5362.480 kb on - strand, within BWI76_RS25935 at 5362.480 kb on - strand, within BWI76_RS25935 at 5362.927 kb on - strand at 5362.937 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB31 MOI 1 remove 5,359,521 - BWI76_RS25920 0.13 -2.7 5,359,585 + BWI76_RS25920 0.23 -0.3 5,359,835 - BWI76_RS25920 0.66 -0.7 5,359,993 - -0.9 5,360,185 + BWI76_RS25925 0.28 +1.1 5,360,254 - BWI76_RS25925 0.62 +1.3 5,360,278 - BWI76_RS25925 0.74 +1.7 5,360,425 - -0.4 5,360,450 - -1.0 5,360,628 + BWI76_RS25930 0.16 +0.1 5,360,703 - BWI76_RS25930 0.20 -0.0 5,361,199 + BWI76_RS25930 0.48 +0.5 5,361,259 + BWI76_RS25930 0.51 +2.9 5,361,267 - BWI76_RS25930 0.51 +2.3 5,361,389 + BWI76_RS25930 0.58 +0.9 5,361,407 - BWI76_RS25930 0.59 +0.6 5,361,478 + BWI76_RS25930 0.63 +2.1 5,361,493 + BWI76_RS25930 0.64 -1.0 5,361,542 - BWI76_RS25930 0.67 -0.2 5,361,692 + BWI76_RS25930 0.75 -0.2 5,361,706 - BWI76_RS25930 0.76 +0.4 5,361,724 + BWI76_RS25930 0.77 +0.3 5,361,741 - BWI76_RS25930 0.78 +0.2 5,361,932 - BWI76_RS25930 0.88 +0.8 5,362,003 + -1.2 5,362,211 + BWI76_RS25935 0.14 +1.2 5,362,438 - BWI76_RS25935 0.54 +1.2 5,362,480 - BWI76_RS25935 0.62 +1.1 5,362,480 - BWI76_RS25935 0.62 -0.2 5,362,927 - -1.4 5,362,937 + -2.9
Or see this region's nucleotide sequence