Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS24675

Experiment: KLEB31 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS24670 and BWI76_RS24675 are separated by 219 nucleotidesBWI76_RS24675 and BWI76_RS24680 are separated by 74 nucleotides BWI76_RS24670: BWI76_RS24670 - autoinducer-2 kinase, at 5,141,742 to 5,143,334 _RS24670 BWI76_RS24675: BWI76_RS24675 - NADPH-dependent 2,4-dienoyl-CoA reductase, at 5,143,554 to 5,145,575 _RS24675 BWI76_RS24680: BWI76_RS24680 - 23S rRNA (guanine(1835)-N(2))-methyltransferase, at 5,145,650 to 5,146,780 _RS24680 Position (kb) 5143 5144 5145 5146Strain fitness (log2 ratio) -3 -2 -1 0 1at 5142.859 kb on - strand, within BWI76_RS24670at 5142.885 kb on + strand, within BWI76_RS24670at 5143.057 kb on + strand, within BWI76_RS24670at 5143.121 kb on + strand, within BWI76_RS24670at 5143.329 kb on + strandat 5143.329 kb on + strandat 5143.337 kb on - strandat 5143.337 kb on - strandat 5143.337 kb on - strandat 5143.337 kb on - strandat 5143.776 kb on + strand, within BWI76_RS24675at 5143.784 kb on - strand, within BWI76_RS24675at 5143.786 kb on - strand, within BWI76_RS24675at 5143.836 kb on - strand, within BWI76_RS24675at 5144.150 kb on + strand, within BWI76_RS24675at 5144.158 kb on - strand, within BWI76_RS24675at 5144.544 kb on + strand, within BWI76_RS24675at 5144.566 kb on + strand, within BWI76_RS24675at 5144.576 kb on + strand, within BWI76_RS24675at 5144.579 kb on - strand, within BWI76_RS24675at 5144.624 kb on - strand, within BWI76_RS24675at 5144.725 kb on - strand, within BWI76_RS24675at 5144.754 kb on - strand, within BWI76_RS24675at 5144.762 kb on + strand, within BWI76_RS24675at 5145.102 kb on - strand, within BWI76_RS24675at 5145.104 kb on + strand, within BWI76_RS24675at 5145.137 kb on + strand, within BWI76_RS24675at 5145.137 kb on + strand, within BWI76_RS24675at 5145.173 kb on - strand, within BWI76_RS24675at 5145.226 kb on + strand, within BWI76_RS24675at 5145.391 kb on + strandat 5145.484 kb on - strandat 5145.641 kb on + strandat 5145.656 kb on - strandat 5145.667 kb on + strandat 5145.675 kb on + strandat 5145.999 kb on + strand, within BWI76_RS24680at 5146.026 kb on - strand, within BWI76_RS24680at 5146.079 kb on + strand, within BWI76_RS24680at 5146.148 kb on - strand, within BWI76_RS24680at 5146.168 kb on - strand, within BWI76_RS24680at 5146.186 kb on - strand, within BWI76_RS24680at 5146.568 kb on - strand, within BWI76_RS24680at 5146.573 kb on + strand, within BWI76_RS24680

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB31 MOI 1
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5,142,859 - BWI76_RS24670 0.70 +0.3
5,142,885 + BWI76_RS24670 0.72 +0.2
5,143,057 + BWI76_RS24670 0.83 -1.3
5,143,121 + BWI76_RS24670 0.87 +0.9
5,143,329 + -0.6
5,143,329 + -3.2
5,143,337 - +0.2
5,143,337 - +0.5
5,143,337 - +1.4
5,143,337 - -0.0
5,143,776 + BWI76_RS24675 0.11 +0.2
5,143,784 - BWI76_RS24675 0.11 -0.8
5,143,786 - BWI76_RS24675 0.11 +1.0
5,143,836 - BWI76_RS24675 0.14 -0.6
5,144,150 + BWI76_RS24675 0.29 -1.8
5,144,158 - BWI76_RS24675 0.30 -0.3
5,144,544 + BWI76_RS24675 0.49 -0.9
5,144,566 + BWI76_RS24675 0.50 -0.9
5,144,576 + BWI76_RS24675 0.51 -0.3
5,144,579 - BWI76_RS24675 0.51 -0.4
5,144,624 - BWI76_RS24675 0.53 +0.3
5,144,725 - BWI76_RS24675 0.58 +1.5
5,144,754 - BWI76_RS24675 0.59 -1.9
5,144,762 + BWI76_RS24675 0.60 -0.8
5,145,102 - BWI76_RS24675 0.77 -0.4
5,145,104 + BWI76_RS24675 0.77 -0.5
5,145,137 + BWI76_RS24675 0.78 -0.7
5,145,137 + BWI76_RS24675 0.78 -0.2
5,145,173 - BWI76_RS24675 0.80 -1.0
5,145,226 + BWI76_RS24675 0.83 +0.1
5,145,391 + -1.7
5,145,484 - +0.4
5,145,641 + -1.9
5,145,656 - -2.5
5,145,667 + +0.4
5,145,675 + +0.1
5,145,999 + BWI76_RS24680 0.31 +0.2
5,146,026 - BWI76_RS24680 0.33 -1.9
5,146,079 + BWI76_RS24680 0.38 +0.0
5,146,148 - BWI76_RS24680 0.44 -0.2
5,146,168 - BWI76_RS24680 0.46 +0.2
5,146,186 - BWI76_RS24680 0.47 +0.3
5,146,568 - BWI76_RS24680 0.81 -0.5
5,146,573 + BWI76_RS24680 0.82 -2.3

Or see this region's nucleotide sequence