Experiment: KLEB31 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS23775 and BWI76_RS23780 are separated by 12 nucleotides BWI76_RS23780 and BWI76_RS23785 are separated by 14 nucleotides BWI76_RS23785 and BWI76_RS23790 are separated by 52 nucleotides BWI76_RS23790 and BWI76_RS23795 are separated by 42 nucleotides
BWI76_RS23775: BWI76_RS23775 - 3,4-dihydroxybenzoate decarboxylase, at 4,963,127 to 4,964,620
_RS23775
BWI76_RS23780: BWI76_RS23780 - aromatic acid decarboxylase, at 4,964,633 to 4,965,211
_RS23780
BWI76_RS23785: BWI76_RS23785 - hypothetical protein, at 4,965,226 to 4,965,942
_RS23785
BWI76_RS23790: BWI76_RS23790 - isopentenyl-diphosphate Delta-isomerase, at 4,965,995 to 4,966,543
_RS23790
BWI76_RS23795: BWI76_RS23795 - lysine--tRNA ligase, at 4,966,586 to 4,968,103
_RS23795
Position (kb)
4965
4966 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4964.383 kb on + strand, within BWI76_RS23775 at 4964.383 kb on + strand, within BWI76_RS23775 at 4964.383 kb on + strand, within BWI76_RS23775 at 4964.391 kb on - strand, within BWI76_RS23775 at 4964.391 kb on - strand, within BWI76_RS23775 at 4964.391 kb on - strand, within BWI76_RS23775 at 4964.391 kb on - strand, within BWI76_RS23775 at 4964.449 kb on - strand, within BWI76_RS23775 at 4964.454 kb on + strand, within BWI76_RS23775 at 4964.472 kb on + strand at 4964.545 kb on - strand at 4964.583 kb on + strand at 4964.637 kb on - strand at 4964.637 kb on - strand at 4964.657 kb on - strand at 4964.685 kb on + strand at 4964.843 kb on + strand, within BWI76_RS23780 at 4964.848 kb on + strand, within BWI76_RS23780 at 4964.982 kb on + strand, within BWI76_RS23780 at 4965.163 kb on + strand at 4965.178 kb on + strand at 4965.244 kb on - strand at 4965.335 kb on - strand, within BWI76_RS23785 at 4965.343 kb on - strand, within BWI76_RS23785 at 4965.358 kb on - strand, within BWI76_RS23785 at 4965.535 kb on - strand, within BWI76_RS23785 at 4965.688 kb on + strand, within BWI76_RS23785 at 4965.711 kb on - strand, within BWI76_RS23785 at 4966.034 kb on - strand at 4966.036 kb on + strand at 4966.491 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB31 MOI 1 remove 4,964,383 + BWI76_RS23775 0.84 +1.2 4,964,383 + BWI76_RS23775 0.84 +0.8 4,964,383 + BWI76_RS23775 0.84 -0.4 4,964,391 - BWI76_RS23775 0.85 -1.0 4,964,391 - BWI76_RS23775 0.85 +1.6 4,964,391 - BWI76_RS23775 0.85 +0.5 4,964,391 - BWI76_RS23775 0.85 +1.0 4,964,449 - BWI76_RS23775 0.88 -0.1 4,964,454 + BWI76_RS23775 0.89 +0.3 4,964,472 + +0.5 4,964,545 - -0.2 4,964,583 + -0.6 4,964,637 - -2.0 4,964,637 - -2.3 4,964,657 - +0.2 4,964,685 + +2.0 4,964,843 + BWI76_RS23780 0.36 -0.9 4,964,848 + BWI76_RS23780 0.37 +0.8 4,964,982 + BWI76_RS23780 0.60 -0.1 4,965,163 + -0.3 4,965,178 + +0.0 4,965,244 - +1.1 4,965,335 - BWI76_RS23785 0.15 +0.0 4,965,343 - BWI76_RS23785 0.16 -0.7 4,965,358 - BWI76_RS23785 0.18 -0.6 4,965,535 - BWI76_RS23785 0.43 -1.0 4,965,688 + BWI76_RS23785 0.64 +0.0 4,965,711 - BWI76_RS23785 0.68 -1.4 4,966,034 - -0.1 4,966,036 + -1.1 4,966,491 - +0.5
Or see this region's nucleotide sequence