Experiment: KLEB31 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22825 and BWI76_RS22830 are separated by 19 nucleotides BWI76_RS22830 and BWI76_RS22835 overlap by 4 nucleotides
BWI76_RS22825: BWI76_RS22825 - glucarate dehydratase, at 4,762,559 to 4,763,899
_RS22825
BWI76_RS22830: BWI76_RS22830 - glucarate dehydratase, at 4,763,919 to 4,765,259
_RS22830
BWI76_RS22835: BWI76_RS22835 - MFS transporter, at 4,765,256 to 4,766,614
_RS22835
Position (kb) 4763
4764
4765
4766 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4762.947 kb on + strand, within BWI76_RS22825 at 4762.947 kb on + strand, within BWI76_RS22825 at 4762.947 kb on + strand, within BWI76_RS22825 at 4762.947 kb on + strand, within BWI76_RS22825 at 4762.955 kb on - strand, within BWI76_RS22825 at 4763.004 kb on - strand, within BWI76_RS22825 at 4763.005 kb on - strand at 4763.016 kb on - strand, within BWI76_RS22825 at 4763.096 kb on + strand, within BWI76_RS22825 at 4763.109 kb on - strand, within BWI76_RS22825 at 4763.109 kb on - strand, within BWI76_RS22825 at 4763.179 kb on + strand, within BWI76_RS22825 at 4763.981 kb on - strand at 4763.987 kb on - strand at 4764.014 kb on + strand at 4764.022 kb on - strand at 4764.022 kb on - strand at 4764.027 kb on - strand at 4764.032 kb on - strand at 4764.062 kb on - strand, within BWI76_RS22830 at 4764.071 kb on + strand, within BWI76_RS22830 at 4764.071 kb on + strand, within BWI76_RS22830 at 4764.071 kb on + strand, within BWI76_RS22830 at 4764.079 kb on - strand, within BWI76_RS22830 at 4764.253 kb on + strand, within BWI76_RS22830 at 4764.258 kb on + strand, within BWI76_RS22830 at 4764.511 kb on + strand, within BWI76_RS22830 at 4764.519 kb on - strand, within BWI76_RS22830 at 4764.647 kb on + strand, within BWI76_RS22830 at 4764.774 kb on + strand, within BWI76_RS22830 at 4764.802 kb on + strand, within BWI76_RS22830 at 4764.824 kb on - strand, within BWI76_RS22830 at 4764.886 kb on + strand, within BWI76_RS22830 at 4765.109 kb on + strand, within BWI76_RS22830 at 4765.227 kb on - strand at 4765.329 kb on + strand at 4765.330 kb on - strand at 4765.339 kb on + strand at 4765.361 kb on - strand at 4765.451 kb on - strand, within BWI76_RS22835 at 4765.785 kb on + strand, within BWI76_RS22835 at 4765.866 kb on + strand, within BWI76_RS22835 at 4765.938 kb on - strand, within BWI76_RS22835 at 4766.094 kb on - strand, within BWI76_RS22835 at 4766.145 kb on + strand, within BWI76_RS22835 at 4766.169 kb on + strand, within BWI76_RS22835
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB31 MOI 1 remove 4,762,947 + BWI76_RS22825 0.29 +2.7 4,762,947 + BWI76_RS22825 0.29 -0.9 4,762,947 + BWI76_RS22825 0.29 -1.2 4,762,947 + BWI76_RS22825 0.29 +0.3 4,762,955 - BWI76_RS22825 0.30 +0.2 4,763,004 - BWI76_RS22825 0.33 -0.2 4,763,005 - -1.1 4,763,016 - BWI76_RS22825 0.34 -1.1 4,763,096 + BWI76_RS22825 0.40 -0.6 4,763,109 - BWI76_RS22825 0.41 -0.3 4,763,109 - BWI76_RS22825 0.41 +2.0 4,763,179 + BWI76_RS22825 0.46 -0.3 4,763,981 - +0.3 4,763,987 - +1.9 4,764,014 + -2.2 4,764,022 - -0.9 4,764,022 - +1.0 4,764,027 - +0.0 4,764,032 - -0.1 4,764,062 - BWI76_RS22830 0.11 -0.2 4,764,071 + BWI76_RS22830 0.11 +1.8 4,764,071 + BWI76_RS22830 0.11 -2.1 4,764,071 + BWI76_RS22830 0.11 +0.6 4,764,079 - BWI76_RS22830 0.12 +0.1 4,764,253 + BWI76_RS22830 0.25 -0.2 4,764,258 + BWI76_RS22830 0.25 -2.1 4,764,511 + BWI76_RS22830 0.44 +2.6 4,764,519 - BWI76_RS22830 0.45 +0.9 4,764,647 + BWI76_RS22830 0.54 +1.9 4,764,774 + BWI76_RS22830 0.64 +1.8 4,764,802 + BWI76_RS22830 0.66 +0.0 4,764,824 - BWI76_RS22830 0.67 +0.6 4,764,886 + BWI76_RS22830 0.72 +0.6 4,765,109 + BWI76_RS22830 0.89 -0.6 4,765,227 - +0.4 4,765,329 + +0.2 4,765,330 - +0.8 4,765,339 + +0.3 4,765,361 - +0.3 4,765,451 - BWI76_RS22835 0.14 +0.1 4,765,785 + BWI76_RS22835 0.39 +0.7 4,765,866 + BWI76_RS22835 0.45 +0.9 4,765,938 - BWI76_RS22835 0.50 -1.0 4,766,094 - BWI76_RS22835 0.62 -1.5 4,766,145 + BWI76_RS22835 0.65 +1.5 4,766,169 + BWI76_RS22835 0.67 +0.7
Or see this region's nucleotide sequence