Experiment: KLEB31 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20840 and BWI76_RS20845 are separated by 78 nucleotides BWI76_RS20845 and BWI76_RS20850 are separated by 58 nucleotides BWI76_RS20850 and BWI76_RS20855 are separated by 186 nucleotides BWI76_RS20855 and BWI76_RS20860 are separated by 5 nucleotides
BWI76_RS20840: BWI76_RS20840 - hypothetical protein, at 4,356,046 to 4,357,113
_RS20840
BWI76_RS20845: BWI76_RS20845 - GDP-mannose pyrophosphatase NudK, at 4,357,192 to 4,357,776
_RS20845
BWI76_RS20850: BWI76_RS20850 - oxidoreductase FeS-binding subunit, at 4,357,835 to 4,359,799
_RS20850
BWI76_RS20855: BWI76_RS20855 - hypothetical protein, at 4,359,986 to 4,360,166
_RS20855
BWI76_RS20860: BWI76_RS20860 - aminoglycoside/multidrug transporter subunit AcrD, at 4,360,172 to 4,363,285
_RS20860
Position (kb) 4357
4358
4359
4360 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 4356.930 kb on + strand, within BWI76_RS20840 at 4357.582 kb on - strand, within BWI76_RS20845 at 4357.656 kb on - strand, within BWI76_RS20845 at 4357.656 kb on - strand, within BWI76_RS20845 at 4357.807 kb on + strand at 4357.834 kb on - strand at 4358.128 kb on + strand, within BWI76_RS20850 at 4358.624 kb on - strand, within BWI76_RS20850 at 4358.896 kb on - strand, within BWI76_RS20850 at 4358.900 kb on + strand, within BWI76_RS20850 at 4359.006 kb on + strand, within BWI76_RS20850 at 4359.093 kb on + strand, within BWI76_RS20850 at 4359.150 kb on + strand, within BWI76_RS20850 at 4359.155 kb on + strand, within BWI76_RS20850 at 4359.321 kb on + strand, within BWI76_RS20850 at 4359.350 kb on - strand, within BWI76_RS20850 at 4359.636 kb on - strand at 4359.668 kb on - strand at 4359.670 kb on + strand at 4359.742 kb on + strand at 4359.983 kb on + strand at 4360.001 kb on + strand at 4360.009 kb on - strand, within BWI76_RS20855 at 4360.011 kb on + strand, within BWI76_RS20855 at 4360.013 kb on + strand, within BWI76_RS20855 at 4360.025 kb on + strand, within BWI76_RS20855 at 4360.033 kb on + strand, within BWI76_RS20855 at 4360.098 kb on + strand, within BWI76_RS20855 at 4360.100 kb on - strand, within BWI76_RS20855 at 4360.106 kb on - strand, within BWI76_RS20855 at 4360.106 kb on - strand, within BWI76_RS20855 at 4360.118 kb on + strand, within BWI76_RS20855 at 4360.120 kb on + strand, within BWI76_RS20855 at 4360.120 kb on + strand, within BWI76_RS20855 at 4360.120 kb on + strand, within BWI76_RS20855 at 4360.128 kb on - strand, within BWI76_RS20855 at 4360.235 kb on + strand at 4360.275 kb on + strand at 4360.367 kb on + strand at 4360.372 kb on + strand at 4360.417 kb on - strand at 4360.471 kb on + strand at 4360.525 kb on + strand, within BWI76_RS20860 at 4360.583 kb on - strand, within BWI76_RS20860 at 4360.707 kb on - strand, within BWI76_RS20860 at 4360.747 kb on + strand, within BWI76_RS20860
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB31 MOI 1 remove 4,356,930 + BWI76_RS20840 0.83 +0.6 4,357,582 - BWI76_RS20845 0.67 -0.1 4,357,656 - BWI76_RS20845 0.79 -0.1 4,357,656 - BWI76_RS20845 0.79 +0.8 4,357,807 + -1.9 4,357,834 - -0.0 4,358,128 + BWI76_RS20850 0.15 +0.1 4,358,624 - BWI76_RS20850 0.40 -1.5 4,358,896 - BWI76_RS20850 0.54 -1.2 4,358,900 + BWI76_RS20850 0.54 +1.5 4,359,006 + BWI76_RS20850 0.60 -1.5 4,359,093 + BWI76_RS20850 0.64 +0.1 4,359,150 + BWI76_RS20850 0.67 +0.5 4,359,155 + BWI76_RS20850 0.67 -3.6 4,359,321 + BWI76_RS20850 0.76 +0.9 4,359,350 - BWI76_RS20850 0.77 -2.5 4,359,636 - +3.1 4,359,668 - +2.0 4,359,670 + -1.0 4,359,742 + +1.2 4,359,983 + -0.9 4,360,001 + -1.2 4,360,009 - BWI76_RS20855 0.13 -0.1 4,360,011 + BWI76_RS20855 0.14 +0.9 4,360,013 + BWI76_RS20855 0.15 -1.1 4,360,025 + BWI76_RS20855 0.22 -0.3 4,360,033 + BWI76_RS20855 0.26 +0.7 4,360,098 + BWI76_RS20855 0.62 +1.2 4,360,100 - BWI76_RS20855 0.63 +3.5 4,360,106 - BWI76_RS20855 0.66 +0.3 4,360,106 - BWI76_RS20855 0.66 -0.7 4,360,118 + BWI76_RS20855 0.73 -0.9 4,360,120 + BWI76_RS20855 0.74 -0.2 4,360,120 + BWI76_RS20855 0.74 -2.1 4,360,120 + BWI76_RS20855 0.74 -0.5 4,360,128 - BWI76_RS20855 0.78 -0.1 4,360,235 + -0.8 4,360,275 + -1.4 4,360,367 + +0.3 4,360,372 + -1.8 4,360,417 - -1.6 4,360,471 + -0.4 4,360,525 + BWI76_RS20860 0.11 +0.1 4,360,583 - BWI76_RS20860 0.13 -0.4 4,360,707 - BWI76_RS20860 0.17 +0.0 4,360,747 + BWI76_RS20860 0.18 -0.1
Or see this region's nucleotide sequence