Experiment: KLEB31 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20660 and BWI76_RS20665 overlap by 4 nucleotides BWI76_RS20665 and BWI76_RS20670 are separated by 82 nucleotides BWI76_RS20670 and BWI76_RS20675 are separated by 14 nucleotides BWI76_RS20675 and BWI76_RS20680 are separated by 45 nucleotides
BWI76_RS20660: BWI76_RS20660 - hypothetical protein, at 4,323,185 to 4,323,424
_RS20660
BWI76_RS20665: BWI76_RS20665 - bifunctional pyridoxal kinase/hydroxymethylpyrimidine kinase, at 4,323,421 to 4,324,287
_RS20665
BWI76_RS20670: BWI76_RS20670 - transcriptional regulator PtsJ, at 4,324,370 to 4,325,668
_RS20670
BWI76_RS20675: BWI76_RS20675 - GMP synthase, at 4,325,683 to 4,326,390
_RS20675
BWI76_RS20680: BWI76_RS20680 - hypothetical protein, at 4,326,436 to 4,326,840
_RS20680
Position (kb) 4324
4325
4326 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4323.795 kb on + strand, within BWI76_RS20665 at 4323.959 kb on + strand, within BWI76_RS20665 at 4323.967 kb on - strand, within BWI76_RS20665 at 4324.019 kb on + strand, within BWI76_RS20665 at 4324.114 kb on - strand, within BWI76_RS20665 at 4324.152 kb on + strand, within BWI76_RS20665 at 4324.189 kb on + strand, within BWI76_RS20665 at 4324.220 kb on + strand at 4324.515 kb on + strand, within BWI76_RS20670 at 4324.533 kb on + strand, within BWI76_RS20670 at 4325.054 kb on - strand, within BWI76_RS20670 at 4325.140 kb on + strand, within BWI76_RS20670 at 4325.549 kb on + strand at 4325.701 kb on - strand at 4325.726 kb on + strand at 4325.731 kb on + strand at 4325.779 kb on - strand, within BWI76_RS20675 at 4325.864 kb on + strand, within BWI76_RS20675 at 4325.904 kb on + strand, within BWI76_RS20675 at 4326.249 kb on + strand, within BWI76_RS20675 at 4326.249 kb on + strand at 4326.258 kb on - strand, within BWI76_RS20675 at 4326.315 kb on + strand, within BWI76_RS20675
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB31 MOI 1 remove 4,323,795 + BWI76_RS20665 0.43 -0.5 4,323,959 + BWI76_RS20665 0.62 -0.3 4,323,967 - BWI76_RS20665 0.63 -0.7 4,324,019 + BWI76_RS20665 0.69 +0.4 4,324,114 - BWI76_RS20665 0.80 +0.7 4,324,152 + BWI76_RS20665 0.84 -1.6 4,324,189 + BWI76_RS20665 0.89 -0.8 4,324,220 + +0.9 4,324,515 + BWI76_RS20670 0.11 +1.1 4,324,533 + BWI76_RS20670 0.13 +1.6 4,325,054 - BWI76_RS20670 0.53 +0.9 4,325,140 + BWI76_RS20670 0.59 +0.3 4,325,549 + +0.4 4,325,701 - -3.4 4,325,726 + -1.8 4,325,731 + -1.9 4,325,779 - BWI76_RS20675 0.14 +0.2 4,325,864 + BWI76_RS20675 0.26 -1.6 4,325,904 + BWI76_RS20675 0.31 -1.9 4,326,249 + BWI76_RS20675 0.80 +0.3 4,326,249 + -1.4 4,326,258 - BWI76_RS20675 0.81 +0.5 4,326,315 + BWI76_RS20675 0.89 +1.9
Or see this region's nucleotide sequence