Experiment: KLEB31 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20150 and BWI76_RS20155 are separated by 10 nucleotides BWI76_RS20155 and BWI76_RS20160 are separated by 14 nucleotides BWI76_RS20160 and BWI76_RS20165 overlap by 4 nucleotides
BWI76_RS20150: BWI76_RS20150 - NADH:ubiquinone oxidoreductase subunit J, at 4,222,275 to 4,222,829
_RS20150
BWI76_RS20155: BWI76_RS20155 - NADH-quinone oxidoreductase subunit I, at 4,222,840 to 4,223,382
_RS20155
BWI76_RS20160: BWI76_RS20160 - NADH-quinone oxidoreductase subunit H, at 4,223,397 to 4,224,374
_RS20160
BWI76_RS20165: BWI76_RS20165 - NADH-quinone oxidoreductase subunit G, at 4,224,371 to 4,227,097
_RS20165
Position (kb)
4223
4224
4225 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4222.604 kb on + strand, within BWI76_RS20150 at 4222.645 kb on - strand, within BWI76_RS20150 at 4222.756 kb on + strand, within BWI76_RS20150 at 4222.891 kb on + strand at 4222.899 kb on - strand, within BWI76_RS20155 at 4223.157 kb on + strand, within BWI76_RS20155 at 4223.190 kb on + strand, within BWI76_RS20155 at 4223.388 kb on + strand at 4223.447 kb on + strand at 4223.659 kb on + strand, within BWI76_RS20160 at 4223.804 kb on - strand, within BWI76_RS20160 at 4224.021 kb on + strand, within BWI76_RS20160 at 4224.218 kb on - strand, within BWI76_RS20160 at 4224.587 kb on - strand at 4225.133 kb on - strand, within BWI76_RS20165
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB31 MOI 1 remove 4,222,604 + BWI76_RS20150 0.59 +1.7 4,222,645 - BWI76_RS20150 0.67 +1.8 4,222,756 + BWI76_RS20150 0.87 -0.1 4,222,891 + -0.0 4,222,899 - BWI76_RS20155 0.11 -0.5 4,223,157 + BWI76_RS20155 0.58 +0.2 4,223,190 + BWI76_RS20155 0.64 +2.3 4,223,388 + +0.1 4,223,447 + -0.4 4,223,659 + BWI76_RS20160 0.27 -1.2 4,223,804 - BWI76_RS20160 0.42 -0.2 4,224,021 + BWI76_RS20160 0.64 -2.2 4,224,218 - BWI76_RS20160 0.84 -2.0 4,224,587 - +0.4 4,225,133 - BWI76_RS20165 0.28 +0.3
Or see this region's nucleotide sequence