Experiment: KLEB31 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16300 and BWI76_RS16305 are separated by 315 nucleotides BWI76_RS16305 and BWI76_RS16310 are separated by 188 nucleotides BWI76_RS16310 and BWI76_RS16315 overlap by 4 nucleotides BWI76_RS16315 and BWI76_RS16320 are separated by 77 nucleotides
BWI76_RS16300: BWI76_RS16300 - bifunctional beta-cystathionase/maltose regulon regulatory protein, at 3,384,179 to 3,385,357
_RS16300
BWI76_RS16305: BWI76_RS16305 - selenium-binding protein YdfZ, at 3,385,673 to 3,385,876
_RS16305
BWI76_RS16310: BWI76_RS16310 - hypothetical protein, at 3,386,065 to 3,387,276
_RS16310
BWI76_RS16315: BWI76_RS16315 - SirA-like protein, at 3,387,273 to 3,387,506
_RS16315
BWI76_RS16320: BWI76_RS16320 - methionine ABC transporter substrate-binding protein, at 3,387,584 to 3,388,396
_RS16320
Position (kb)
3386
3387
3388 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3385.327 kb on - strand at 3385.372 kb on + strand at 3385.372 kb on + strand at 3385.380 kb on - strand at 3385.380 kb on - strand at 3385.380 kb on - strand at 3385.380 kb on - strand at 3385.474 kb on + strand at 3385.539 kb on + strand at 3385.547 kb on - strand at 3385.574 kb on + strand at 3385.693 kb on + strand at 3385.693 kb on + strand at 3385.693 kb on + strand at 3385.693 kb on + strand at 3385.699 kb on + strand, within BWI76_RS16305 at 3385.701 kb on - strand, within BWI76_RS16305 at 3385.727 kb on - strand, within BWI76_RS16305 at 3385.727 kb on - strand, within BWI76_RS16305 at 3385.742 kb on - strand, within BWI76_RS16305 at 3385.751 kb on - strand, within BWI76_RS16305 at 3385.761 kb on - strand, within BWI76_RS16305 at 3385.774 kb on + strand, within BWI76_RS16305 at 3385.782 kb on - strand, within BWI76_RS16305 at 3385.787 kb on + strand at 3385.787 kb on + strand, within BWI76_RS16305 at 3385.792 kb on + strand, within BWI76_RS16305 at 3385.792 kb on + strand, within BWI76_RS16305 at 3385.795 kb on - strand, within BWI76_RS16305 at 3385.865 kb on + strand at 3385.865 kb on + strand at 3385.865 kb on + strand at 3385.867 kb on - strand at 3385.880 kb on + strand at 3385.891 kb on + strand at 3385.891 kb on + strand at 3385.891 kb on + strand at 3385.891 kb on + strand at 3385.899 kb on - strand at 3385.899 kb on - strand at 3385.916 kb on + strand at 3385.924 kb on + strand at 3385.925 kb on + strand at 3385.925 kb on + strand at 3385.927 kb on + strand at 3385.932 kb on - strand at 3385.934 kb on + strand at 3385.937 kb on - strand at 3385.983 kb on - strand at 3386.031 kb on + strand at 3386.033 kb on + strand at 3386.069 kb on + strand at 3386.069 kb on - strand at 3386.069 kb on - strand at 3386.079 kb on + strand at 3386.086 kb on - strand at 3386.087 kb on - strand at 3386.087 kb on - strand at 3386.143 kb on + strand at 3386.151 kb on - strand at 3386.157 kb on - strand at 3386.157 kb on - strand at 3386.243 kb on + strand, within BWI76_RS16310 at 3386.266 kb on - strand at 3386.295 kb on - strand, within BWI76_RS16310 at 3386.333 kb on - strand, within BWI76_RS16310 at 3386.414 kb on - strand, within BWI76_RS16310 at 3386.562 kb on - strand, within BWI76_RS16310 at 3387.081 kb on + strand, within BWI76_RS16310 at 3387.098 kb on + strand, within BWI76_RS16310 at 3387.098 kb on + strand, within BWI76_RS16310 at 3387.143 kb on + strand, within BWI76_RS16310 at 3387.445 kb on + strand, within BWI76_RS16315 at 3387.455 kb on + strand, within BWI76_RS16315 at 3388.085 kb on - strand, within BWI76_RS16320 at 3388.101 kb on - strand, within BWI76_RS16320 at 3388.101 kb on - strand at 3388.136 kb on + strand, within BWI76_RS16320 at 3388.187 kb on - strand, within BWI76_RS16320 at 3388.187 kb on - strand, within BWI76_RS16320 at 3388.187 kb on - strand, within BWI76_RS16320 at 3388.219 kb on + strand, within BWI76_RS16320
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB31 MOI 1 remove 3,385,327 - +0.3 3,385,372 + +1.6 3,385,372 + +2.5 3,385,380 - +0.3 3,385,380 - +0.4 3,385,380 - -0.2 3,385,380 - +0.3 3,385,474 + -0.1 3,385,539 + +0.1 3,385,547 - -0.5 3,385,574 + +0.1 3,385,693 + +3.1 3,385,693 + +1.4 3,385,693 + -1.1 3,385,693 + +0.1 3,385,699 + BWI76_RS16305 0.13 -0.4 3,385,701 - BWI76_RS16305 0.14 -0.5 3,385,727 - BWI76_RS16305 0.26 +0.9 3,385,727 - BWI76_RS16305 0.26 +0.5 3,385,742 - BWI76_RS16305 0.34 -0.0 3,385,751 - BWI76_RS16305 0.38 +1.3 3,385,761 - BWI76_RS16305 0.43 -0.6 3,385,774 + BWI76_RS16305 0.50 -0.3 3,385,782 - BWI76_RS16305 0.53 -0.8 3,385,787 + +1.1 3,385,787 + BWI76_RS16305 0.56 -0.5 3,385,792 + BWI76_RS16305 0.58 -0.9 3,385,792 + BWI76_RS16305 0.58 +0.1 3,385,795 - BWI76_RS16305 0.60 -0.6 3,385,865 + -0.5 3,385,865 + -0.2 3,385,865 + -1.1 3,385,867 - +1.0 3,385,880 + -0.3 3,385,891 + +0.4 3,385,891 + -0.5 3,385,891 + -0.5 3,385,891 + +0.9 3,385,899 - -1.0 3,385,899 - +1.2 3,385,916 + -0.6 3,385,924 + +2.3 3,385,925 + +0.5 3,385,925 + +0.2 3,385,927 + -2.3 3,385,932 - -2.8 3,385,934 + +0.1 3,385,937 - +0.1 3,385,983 - +1.5 3,386,031 + -0.5 3,386,033 + +2.2 3,386,069 + +0.8 3,386,069 - +0.1 3,386,069 - +0.1 3,386,079 + +0.5 3,386,086 - -0.4 3,386,087 - +0.3 3,386,087 - +0.1 3,386,143 + +0.2 3,386,151 - -0.4 3,386,157 - -0.3 3,386,157 - +0.1 3,386,243 + BWI76_RS16310 0.15 +0.6 3,386,266 - -0.5 3,386,295 - BWI76_RS16310 0.19 +1.2 3,386,333 - BWI76_RS16310 0.22 -0.3 3,386,414 - BWI76_RS16310 0.29 +1.4 3,386,562 - BWI76_RS16310 0.41 +2.3 3,387,081 + BWI76_RS16310 0.84 +0.7 3,387,098 + BWI76_RS16310 0.85 +3.2 3,387,098 + BWI76_RS16310 0.85 +0.2 3,387,143 + BWI76_RS16310 0.89 +2.4 3,387,445 + BWI76_RS16315 0.74 -0.8 3,387,455 + BWI76_RS16315 0.78 +1.5 3,388,085 - BWI76_RS16320 0.62 -2.5 3,388,101 - BWI76_RS16320 0.64 +1.0 3,388,101 - +2.0 3,388,136 + BWI76_RS16320 0.68 +0.5 3,388,187 - BWI76_RS16320 0.74 -1.1 3,388,187 - BWI76_RS16320 0.74 -0.2 3,388,187 - BWI76_RS16320 0.74 +0.4 3,388,219 + BWI76_RS16320 0.78 -1.8
Or see this region's nucleotide sequence