Experiment: KLEB31 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14180 and BWI76_RS14185 are separated by 1 nucleotides BWI76_RS14185 and BWI76_RS14190 overlap by 4 nucleotides BWI76_RS14190 and BWI76_RS14195 are separated by 128 nucleotides
BWI76_RS14180: BWI76_RS14180 - hypothetical protein, at 2,967,949 to 2,969,034
_RS14180
BWI76_RS14185: BWI76_RS14185 - 4-carboxy-4-hydroxy-2-oxoadipate aldolase/oxaloacetate decarboxylase, at 2,969,036 to 2,969,749
_RS14185
BWI76_RS14190: BWI76_RS14190 - DNA-binding protein, at 2,969,746 to 2,970,486
_RS14190
BWI76_RS14195: BWI76_RS14195 - LysR family transcriptional regulator, at 2,970,615 to 2,971,838
_RS14195
Position (kb)
2969
2970 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2968.229 kb on - strand, within BWI76_RS14180 at 2968.331 kb on - strand, within BWI76_RS14180 at 2968.336 kb on - strand, within BWI76_RS14180 at 2968.401 kb on - strand, within BWI76_RS14180 at 2968.507 kb on - strand, within BWI76_RS14180 at 2968.519 kb on + strand, within BWI76_RS14180 at 2968.603 kb on - strand, within BWI76_RS14180 at 2968.694 kb on - strand, within BWI76_RS14180 at 2968.955 kb on + strand at 2969.534 kb on - strand, within BWI76_RS14185 at 2969.563 kb on - strand, within BWI76_RS14185 at 2969.593 kb on - strand, within BWI76_RS14185 at 2969.593 kb on - strand at 2969.593 kb on - strand, within BWI76_RS14185 at 2969.599 kb on - strand, within BWI76_RS14185 at 2969.670 kb on + strand, within BWI76_RS14185 at 2969.712 kb on - strand at 2969.992 kb on - strand, within BWI76_RS14190 at 2970.301 kb on - strand, within BWI76_RS14190 at 2970.346 kb on + strand, within BWI76_RS14190 at 2970.382 kb on + strand, within BWI76_RS14190 at 2970.393 kb on + strand, within BWI76_RS14190 at 2970.395 kb on - strand, within BWI76_RS14190 at 2970.395 kb on - strand, within BWI76_RS14190 at 2970.399 kb on + strand, within BWI76_RS14190 at 2970.402 kb on - strand, within BWI76_RS14190 at 2970.402 kb on - strand, within BWI76_RS14190 at 2970.402 kb on - strand, within BWI76_RS14190 at 2970.407 kb on - strand, within BWI76_RS14190 at 2970.438 kb on + strand at 2970.446 kb on - strand at 2970.448 kb on + strand at 2970.477 kb on + strand at 2970.480 kb on - strand at 2970.484 kb on + strand at 2970.484 kb on + strand at 2970.492 kb on - strand at 2970.492 kb on - strand at 2970.495 kb on + strand at 2970.495 kb on + strand at 2970.503 kb on - strand at 2970.518 kb on + strand at 2970.518 kb on + strand at 2970.518 kb on + strand at 2970.518 kb on + strand at 2970.518 kb on + strand at 2970.526 kb on - strand at 2970.526 kb on - strand at 2970.526 kb on - strand at 2970.526 kb on - strand at 2970.538 kb on + strand at 2970.678 kb on + strand at 2970.678 kb on + strand at 2970.685 kb on - strand at 2970.686 kb on + strand at 2970.686 kb on - strand at 2970.687 kb on - strand at 2970.696 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB31 MOI 1 remove 2,968,229 - BWI76_RS14180 0.26 -0.2 2,968,331 - BWI76_RS14180 0.35 -1.4 2,968,336 - BWI76_RS14180 0.36 -1.5 2,968,401 - BWI76_RS14180 0.42 +0.8 2,968,507 - BWI76_RS14180 0.51 -0.9 2,968,519 + BWI76_RS14180 0.52 +2.2 2,968,603 - BWI76_RS14180 0.60 +0.6 2,968,694 - BWI76_RS14180 0.69 +0.3 2,968,955 + +0.7 2,969,534 - BWI76_RS14185 0.70 -0.1 2,969,563 - BWI76_RS14185 0.74 +0.9 2,969,593 - BWI76_RS14185 0.78 +1.6 2,969,593 - +1.1 2,969,593 - BWI76_RS14185 0.78 -0.5 2,969,599 - BWI76_RS14185 0.79 -0.9 2,969,670 + BWI76_RS14185 0.89 +1.9 2,969,712 - -0.5 2,969,992 - BWI76_RS14190 0.33 +0.1 2,970,301 - BWI76_RS14190 0.75 -0.9 2,970,346 + BWI76_RS14190 0.81 -1.5 2,970,382 + BWI76_RS14190 0.86 -1.7 2,970,393 + BWI76_RS14190 0.87 +0.3 2,970,395 - BWI76_RS14190 0.88 +2.1 2,970,395 - BWI76_RS14190 0.88 +0.8 2,970,399 + BWI76_RS14190 0.88 -0.3 2,970,402 - BWI76_RS14190 0.89 -0.6 2,970,402 - BWI76_RS14190 0.89 -1.1 2,970,402 - BWI76_RS14190 0.89 -1.8 2,970,407 - BWI76_RS14190 0.89 -0.2 2,970,438 + -0.5 2,970,446 - -0.1 2,970,448 + -1.4 2,970,477 + +1.0 2,970,480 - -2.0 2,970,484 + -0.1 2,970,484 + -0.6 2,970,492 - -1.1 2,970,492 - -0.1 2,970,495 + -0.5 2,970,495 + -0.7 2,970,503 - +0.1 2,970,518 + -1.9 2,970,518 + -3.1 2,970,518 + -0.5 2,970,518 + -2.1 2,970,518 + -1.1 2,970,526 - +0.2 2,970,526 - -0.1 2,970,526 - -0.1 2,970,526 - +0.7 2,970,538 + -0.4 2,970,678 + -1.1 2,970,678 + -0.9 2,970,685 - +3.4 2,970,686 + +0.2 2,970,686 - -2.0 2,970,687 - -1.3 2,970,696 - -1.2
Or see this region's nucleotide sequence