Experiment: KLEB31 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12940 and BWI76_RS12945 are separated by 57 nucleotides BWI76_RS12945 and BWI76_RS12950 are separated by 334 nucleotides BWI76_RS12950 and BWI76_RS12955 are separated by 23 nucleotides
BWI76_RS12940: BWI76_RS12940 - AraC family transcriptional regulator, at 2,696,036 to 2,696,857
_RS12940
BWI76_RS12945: BWI76_RS12945 - AraC family transcriptional regulator, at 2,696,915 to 2,697,310
_RS12945
BWI76_RS12950: BWI76_RS12950 - acriflavin resistance protein AcrA, at 2,697,645 to 2,698,820
_RS12950
BWI76_RS12955: BWI76_RS12955 - multidrug efflux RND transporter permease subunit OqxB, at 2,698,844 to 2,701,996
_RS12955
Position (kb)
2697
2698
2699 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 2696.777 kb on - strand at 2696.859 kb on - strand at 2696.859 kb on - strand at 2697.835 kb on - strand, within BWI76_RS12950 at 2698.088 kb on - strand, within BWI76_RS12950 at 2698.297 kb on + strand, within BWI76_RS12950 at 2699.211 kb on - strand, within BWI76_RS12955 at 2699.420 kb on - strand, within BWI76_RS12955 at 2699.643 kb on - strand, within BWI76_RS12955
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB31 MOI 1 remove 2,696,777 - -1.2 2,696,859 - -1.5 2,696,859 - +0.7 2,697,835 - BWI76_RS12950 0.16 +4.4 2,698,088 - BWI76_RS12950 0.38 +1.1 2,698,297 + BWI76_RS12950 0.55 -0.2 2,699,211 - BWI76_RS12955 0.12 +4.4 2,699,420 - BWI76_RS12955 0.18 -0.5 2,699,643 - BWI76_RS12955 0.25 +0.7
Or see this region's nucleotide sequence