Experiment: KLEB31 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12775 and BWI76_RS12780 are separated by 229 nucleotides BWI76_RS12780 and BWI76_RS12785 overlap by 4 nucleotides BWI76_RS12785 and BWI76_RS12790 are separated by 125 nucleotides
BWI76_RS12775: BWI76_RS12775 - hypothetical protein, at 2,663,881 to 2,664,060
_RS12775
BWI76_RS12780: BWI76_RS12780 - hypothetical protein, at 2,664,290 to 2,664,562
_RS12780
BWI76_RS12785: BWI76_RS12785 - heat-shock protein HslJ, at 2,664,559 to 2,664,999
_RS12785
BWI76_RS12790: BWI76_RS12790 - lactate dehydrogenase, at 2,665,125 to 2,666,114
_RS12790
Position (kb)
2664
2665 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2663.595 kb on + strand at 2663.640 kb on + strand at 2664.030 kb on - strand, within BWI76_RS12775 at 2664.060 kb on + strand at 2664.060 kb on + strand at 2664.062 kb on + strand at 2664.067 kb on + strand at 2664.092 kb on + strand at 2664.127 kb on + strand at 2664.137 kb on + strand at 2664.137 kb on + strand at 2664.137 kb on - strand at 2664.138 kb on + strand at 2664.145 kb on - strand at 2664.152 kb on - strand at 2664.193 kb on + strand at 2664.220 kb on + strand at 2664.227 kb on + strand at 2664.235 kb on - strand at 2664.333 kb on - strand, within BWI76_RS12780 at 2664.409 kb on + strand, within BWI76_RS12780 at 2664.665 kb on - strand, within BWI76_RS12785 at 2664.706 kb on + strand, within BWI76_RS12785 at 2664.915 kb on + strand, within BWI76_RS12785 at 2664.923 kb on - strand, within BWI76_RS12785 at 2664.923 kb on - strand, within BWI76_RS12785 at 2664.928 kb on - strand, within BWI76_RS12785 at 2665.775 kb on + strand, within BWI76_RS12790
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB31 MOI 1 remove 2,663,595 + -2.0 2,663,640 + -0.0 2,664,030 - BWI76_RS12775 0.83 -0.0 2,664,060 + +0.1 2,664,060 + +2.6 2,664,062 + -0.0 2,664,067 + -0.3 2,664,092 + +1.8 2,664,127 + -2.0 2,664,137 + +0.3 2,664,137 + -1.0 2,664,137 - -0.3 2,664,138 + +2.2 2,664,145 - -0.0 2,664,152 - -1.0 2,664,193 + -0.1 2,664,220 + -1.8 2,664,227 + -0.3 2,664,235 - +1.8 2,664,333 - BWI76_RS12780 0.16 -0.3 2,664,409 + BWI76_RS12780 0.44 +0.3 2,664,665 - BWI76_RS12785 0.24 +2.8 2,664,706 + BWI76_RS12785 0.33 +2.7 2,664,915 + BWI76_RS12785 0.81 +0.3 2,664,923 - BWI76_RS12785 0.83 -0.3 2,664,923 - BWI76_RS12785 0.83 +0.2 2,664,928 - BWI76_RS12785 0.84 +3.3 2,665,775 + BWI76_RS12790 0.66 +0.5
Or see this region's nucleotide sequence