Experiment: KLEB31 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12630 and BWI76_RS12635 are separated by 690 nucleotides BWI76_RS12635 and BWI76_RS12640 are separated by 216 nucleotides BWI76_RS12640 and BWI76_RS12645 are separated by 125 nucleotides
BWI76_RS12630: BWI76_RS12630 - regulatory protein, LysR:LysR, substrate-binding protein, at 2,636,172 to 2,637,077
_RS12630
BWI76_RS12635: BWI76_RS12635 - cold-shock protein, at 2,637,768 to 2,637,956
_RS12635
BWI76_RS12640: BWI76_RS12640 - hypothetical protein, at 2,638,173 to 2,638,802
_RS12640
BWI76_RS12645: BWI76_RS12645 - membrane protein, at 2,638,928 to 2,639,509
_RS12645
Position (kb)
2637
2638 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2636.854 kb on + strand, within BWI76_RS12630 at 2636.862 kb on - strand, within BWI76_RS12630 at 2636.975 kb on - strand, within BWI76_RS12630 at 2637.033 kb on + strand at 2637.139 kb on - strand at 2637.139 kb on - strand at 2637.141 kb on + strand at 2637.171 kb on - strand at 2637.249 kb on + strand at 2637.275 kb on + strand at 2637.299 kb on - strand at 2637.375 kb on + strand at 2637.375 kb on + strand at 2637.375 kb on + strand at 2637.375 kb on + strand at 2637.375 kb on + strand at 2637.375 kb on + strand at 2637.375 kb on + strand at 2637.375 kb on + strand at 2637.375 kb on + strand at 2637.375 kb on + strand at 2637.375 kb on + strand at 2637.375 kb on + strand at 2637.375 kb on + strand at 2637.375 kb on + strand at 2637.375 kb on + strand at 2637.375 kb on + strand at 2637.383 kb on - strand at 2637.383 kb on - strand at 2637.383 kb on - strand at 2637.383 kb on - strand at 2637.383 kb on - strand at 2637.383 kb on - strand at 2637.394 kb on + strand at 2637.397 kb on - strand at 2637.432 kb on + strand at 2637.471 kb on - strand at 2637.478 kb on - strand at 2637.520 kb on - strand at 2637.533 kb on + strand at 2637.589 kb on + strand at 2637.589 kb on + strand at 2637.602 kb on + strand at 2637.610 kb on - strand at 2637.613 kb on + strand at 2637.613 kb on + strand at 2637.646 kb on + strand at 2637.656 kb on + strand at 2637.717 kb on + strand at 2637.758 kb on + strand at 2637.764 kb on + strand at 2637.772 kb on - strand at 2637.772 kb on - strand at 2637.772 kb on - strand at 2637.802 kb on + strand, within BWI76_RS12635 at 2637.802 kb on + strand, within BWI76_RS12635 at 2637.802 kb on + strand, within BWI76_RS12635 at 2637.802 kb on - strand, within BWI76_RS12635 at 2637.810 kb on + strand, within BWI76_RS12635 at 2637.810 kb on - strand, within BWI76_RS12635 at 2637.810 kb on - strand at 2637.810 kb on - strand, within BWI76_RS12635 at 2637.813 kb on + strand, within BWI76_RS12635 at 2637.815 kb on + strand, within BWI76_RS12635 at 2637.815 kb on + strand, within BWI76_RS12635 at 2637.815 kb on + strand, within BWI76_RS12635 at 2637.815 kb on + strand, within BWI76_RS12635 at 2637.815 kb on + strand, within BWI76_RS12635 at 2637.815 kb on + strand at 2637.815 kb on + strand, within BWI76_RS12635 at 2637.815 kb on + strand at 2637.815 kb on + strand, within BWI76_RS12635 at 2637.815 kb on + strand, within BWI76_RS12635 at 2637.815 kb on + strand at 2637.815 kb on + strand, within BWI76_RS12635 at 2637.815 kb on + strand at 2637.815 kb on + strand, within BWI76_RS12635 at 2637.815 kb on + strand, within BWI76_RS12635 at 2637.815 kb on + strand, within BWI76_RS12635 at 2637.815 kb on + strand, within BWI76_RS12635 at 2637.815 kb on + strand, within BWI76_RS12635 at 2637.815 kb on + strand, within BWI76_RS12635 at 2637.815 kb on + strand, within BWI76_RS12635 at 2637.815 kb on + strand, within BWI76_RS12635 at 2637.815 kb on + strand, within BWI76_RS12635 at 2637.815 kb on + strand, within BWI76_RS12635 at 2637.823 kb on - strand, within BWI76_RS12635 at 2637.823 kb on - strand, within BWI76_RS12635 at 2637.823 kb on - strand, within BWI76_RS12635 at 2637.825 kb on - strand, within BWI76_RS12635 at 2638.023 kb on + strand at 2638.094 kb on - strand at 2638.097 kb on + strand at 2638.173 kb on + strand at 2638.214 kb on + strand at 2638.214 kb on + strand at 2638.285 kb on - strand, within BWI76_RS12640 at 2638.337 kb on + strand, within BWI76_RS12640 at 2638.344 kb on + strand, within BWI76_RS12640 at 2638.352 kb on - strand, within BWI76_RS12640 at 2638.352 kb on - strand, within BWI76_RS12640 at 2638.352 kb on - strand, within BWI76_RS12640 at 2638.401 kb on - strand, within BWI76_RS12640 at 2638.423 kb on + strand, within BWI76_RS12640 at 2638.423 kb on + strand, within BWI76_RS12640 at 2638.436 kb on + strand, within BWI76_RS12640 at 2638.450 kb on + strand, within BWI76_RS12640 at 2638.458 kb on - strand, within BWI76_RS12640 at 2638.458 kb on - strand at 2638.546 kb on - strand, within BWI76_RS12640 at 2638.594 kb on - strand, within BWI76_RS12640 at 2638.638 kb on - strand, within BWI76_RS12640
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB31 MOI 1 remove 2,636,854 + BWI76_RS12630 0.75 +1.3 2,636,862 - BWI76_RS12630 0.76 -0.9 2,636,975 - BWI76_RS12630 0.89 +2.3 2,637,033 + +0.2 2,637,139 - +1.6 2,637,139 - -1.0 2,637,141 + -0.0 2,637,171 - -2.0 2,637,249 + +1.0 2,637,275 + -1.0 2,637,299 - -1.6 2,637,375 + +2.0 2,637,375 + +0.4 2,637,375 + -0.0 2,637,375 + -0.8 2,637,375 + +3.5 2,637,375 + -1.6 2,637,375 + +1.4 2,637,375 + +0.5 2,637,375 + -3.2 2,637,375 + -1.2 2,637,375 + -1.3 2,637,375 + -0.7 2,637,375 + -0.8 2,637,375 + +1.1 2,637,375 + +0.4 2,637,375 + +1.2 2,637,383 - -0.0 2,637,383 - -0.6 2,637,383 - +1.0 2,637,383 - +0.6 2,637,383 - -0.8 2,637,383 - +1.4 2,637,394 + -0.1 2,637,397 - +0.6 2,637,432 + -0.2 2,637,471 - +3.8 2,637,478 - +3.8 2,637,520 - +1.0 2,637,533 + -0.7 2,637,589 + -0.7 2,637,589 + -0.6 2,637,602 + +0.9 2,637,610 - +2.0 2,637,613 + +0.1 2,637,613 + +1.5 2,637,646 + +1.3 2,637,656 + -1.0 2,637,717 + -0.4 2,637,758 + -1.3 2,637,764 + -1.1 2,637,772 - +0.6 2,637,772 - -0.4 2,637,772 - -0.7 2,637,802 + BWI76_RS12635 0.18 +2.0 2,637,802 + BWI76_RS12635 0.18 -0.0 2,637,802 + BWI76_RS12635 0.18 -1.0 2,637,802 - BWI76_RS12635 0.18 -1.3 2,637,810 + BWI76_RS12635 0.22 +0.5 2,637,810 - BWI76_RS12635 0.22 +1.0 2,637,810 - +1.0 2,637,810 - BWI76_RS12635 0.22 -0.0 2,637,813 + BWI76_RS12635 0.24 -0.6 2,637,815 + BWI76_RS12635 0.25 +1.0 2,637,815 + BWI76_RS12635 0.25 +0.7 2,637,815 + BWI76_RS12635 0.25 +2.6 2,637,815 + BWI76_RS12635 0.25 +2.6 2,637,815 + BWI76_RS12635 0.25 +1.0 2,637,815 + -1.0 2,637,815 + BWI76_RS12635 0.25 -2.1 2,637,815 + +0.7 2,637,815 + BWI76_RS12635 0.25 +0.1 2,637,815 + BWI76_RS12635 0.25 +1.2 2,637,815 + -0.6 2,637,815 + BWI76_RS12635 0.25 +0.1 2,637,815 + -0.0 2,637,815 + BWI76_RS12635 0.25 -0.7 2,637,815 + BWI76_RS12635 0.25 -0.3 2,637,815 + BWI76_RS12635 0.25 +1.4 2,637,815 + BWI76_RS12635 0.25 +2.0 2,637,815 + BWI76_RS12635 0.25 -0.0 2,637,815 + BWI76_RS12635 0.25 +0.9 2,637,815 + BWI76_RS12635 0.25 +2.5 2,637,815 + BWI76_RS12635 0.25 +0.4 2,637,815 + BWI76_RS12635 0.25 -0.0 2,637,815 + BWI76_RS12635 0.25 +1.2 2,637,823 - BWI76_RS12635 0.29 +1.0 2,637,823 - BWI76_RS12635 0.29 -0.0 2,637,823 - BWI76_RS12635 0.29 -0.0 2,637,825 - BWI76_RS12635 0.30 -1.8 2,638,023 + +1.0 2,638,094 - +2.7 2,638,097 + -1.0 2,638,173 + -1.3 2,638,214 + -1.3 2,638,214 + -0.0 2,638,285 - BWI76_RS12640 0.18 +1.0 2,638,337 + BWI76_RS12640 0.26 -1.3 2,638,344 + BWI76_RS12640 0.27 +0.4 2,638,352 - BWI76_RS12640 0.28 -0.0 2,638,352 - BWI76_RS12640 0.28 -1.0 2,638,352 - BWI76_RS12640 0.28 +1.8 2,638,401 - BWI76_RS12640 0.36 -0.2 2,638,423 + BWI76_RS12640 0.40 -1.0 2,638,423 + BWI76_RS12640 0.40 +1.0 2,638,436 + BWI76_RS12640 0.42 +0.6 2,638,450 + BWI76_RS12640 0.44 +1.6 2,638,458 - BWI76_RS12640 0.45 +0.4 2,638,458 - -0.0 2,638,546 - BWI76_RS12640 0.59 +0.8 2,638,594 - BWI76_RS12640 0.67 +0.2 2,638,638 - BWI76_RS12640 0.74 +2.6
Or see this region's nucleotide sequence