Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS10640

Experiment: KLEB31 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS10635 and BWI76_RS10640 are separated by 1 nucleotidesBWI76_RS10640 and BWI76_RS10645 are separated by 251 nucleotides BWI76_RS10635: BWI76_RS10635 - GGDEF domain-containing protein, at 2,245,983 to 2,246,885 _RS10635 BWI76_RS10640: BWI76_RS10640 - AraC family transcriptional regulator, at 2,246,887 to 2,247,720 _RS10640 BWI76_RS10645: BWI76_RS10645 - phytanoyl-CoA dioxygenase, at 2,247,972 to 2,248,739 _RS10645 Position (kb) 2246 2247 2248Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2245.893 kb on - strandat 2245.908 kb on + strandat 2245.908 kb on + strandat 2245.914 kb on - strandat 2245.916 kb on - strandat 2245.916 kb on - strandat 2245.916 kb on - strandat 2245.916 kb on - strandat 2245.916 kb on - strandat 2245.916 kb on - strandat 2245.916 kb on - strandat 2245.916 kb on - strandat 2245.916 kb on - strandat 2245.965 kb on + strandat 2245.974 kb on + strandat 2245.977 kb on - strandat 2245.982 kb on - strandat 2246.074 kb on + strand, within BWI76_RS10635at 2246.593 kb on + strand, within BWI76_RS10635at 2246.699 kb on - strand, within BWI76_RS10635at 2246.736 kb on - strand, within BWI76_RS10635at 2246.870 kb on + strandat 2246.992 kb on + strand, within BWI76_RS10640at 2247.121 kb on + strand, within BWI76_RS10640at 2247.148 kb on - strand, within BWI76_RS10640at 2247.575 kb on - strand, within BWI76_RS10640at 2247.644 kb on + strandat 2247.729 kb on + strandat 2247.737 kb on - strandat 2247.922 kb on + strandat 2248.058 kb on + strand, within BWI76_RS10645at 2248.058 kb on + strand, within BWI76_RS10645at 2248.058 kb on + strand, within BWI76_RS10645at 2248.071 kb on - strand, within BWI76_RS10645at 2248.354 kb on + strand, within BWI76_RS10645at 2248.411 kb on + strand, within BWI76_RS10645at 2248.419 kb on - strand, within BWI76_RS10645at 2248.419 kb on - strand, within BWI76_RS10645at 2248.488 kb on + strand, within BWI76_RS10645

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB31 MOI 1
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2,245,893 - +0.9
2,245,908 + +3.4
2,245,908 + +1.7
2,245,914 - +0.8
2,245,916 - -0.5
2,245,916 - +0.4
2,245,916 - -1.2
2,245,916 - -0.8
2,245,916 - -0.9
2,245,916 - -0.4
2,245,916 - -2.3
2,245,916 - +1.4
2,245,916 - +1.0
2,245,965 + -0.3
2,245,974 + -1.0
2,245,977 - +0.6
2,245,982 - -1.0
2,246,074 + BWI76_RS10635 0.10 +0.5
2,246,593 + BWI76_RS10635 0.68 -1.8
2,246,699 - BWI76_RS10635 0.79 +0.8
2,246,736 - BWI76_RS10635 0.83 +0.1
2,246,870 + +0.3
2,246,992 + BWI76_RS10640 0.13 +0.4
2,247,121 + BWI76_RS10640 0.28 +1.7
2,247,148 - BWI76_RS10640 0.31 +0.6
2,247,575 - BWI76_RS10640 0.82 +1.2
2,247,644 + -0.7
2,247,729 + +1.0
2,247,737 - +0.2
2,247,922 + -0.8
2,248,058 + BWI76_RS10645 0.11 +0.4
2,248,058 + BWI76_RS10645 0.11 +0.4
2,248,058 + BWI76_RS10645 0.11 -0.7
2,248,071 - BWI76_RS10645 0.13 -0.2
2,248,354 + BWI76_RS10645 0.50 +1.0
2,248,411 + BWI76_RS10645 0.57 -0.5
2,248,419 - BWI76_RS10645 0.58 -0.2
2,248,419 - BWI76_RS10645 0.58 +1.3
2,248,488 + BWI76_RS10645 0.67 -1.0

Or see this region's nucleotide sequence