Experiment: KLEB31 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10360 and BWI76_RS10365 are separated by 433 nucleotides BWI76_RS10365 and BWI76_RS10370 are separated by 15 nucleotides BWI76_RS10370 and BWI76_RS10375 are separated by 16 nucleotides
BWI76_RS10355: BWI76_RS10355 - spermidine/putrescine ABC transporter, at 2,192,997 to 2,194,092
_RS10355
BWI76_RS10360: BWI76_RS10360 - TetR family transcriptional regulator, at 2,194,068 to 2,194,646
_RS10360
BWI76_RS10365: BWI76_RS10365 - class IV aminotransferase, at 2,195,080 to 2,196,045
_RS10365
BWI76_RS10370: BWI76_RS10370 - ABC transporter-like protein, at 2,196,061 to 2,196,840
_RS10370
BWI76_RS10375: BWI76_RS10375 - polar amino acid ABC transporter inner membrane subunit, at 2,196,857 to 2,197,522
_RS10375
Position (kb)
2195
2196
2197 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2194.443 kb on + strand, within BWI76_RS10360 at 2194.673 kb on - strand at 2194.742 kb on + strand at 2194.883 kb on + strand at 2194.967 kb on + strand at 2195.026 kb on + strand at 2195.139 kb on - strand at 2195.193 kb on + strand, within BWI76_RS10365 at 2195.357 kb on - strand, within BWI76_RS10365 at 2195.586 kb on + strand, within BWI76_RS10365 at 2195.586 kb on + strand, within BWI76_RS10365 at 2195.586 kb on + strand, within BWI76_RS10365 at 2196.102 kb on - strand at 2196.210 kb on + strand, within BWI76_RS10370 at 2196.293 kb on - strand, within BWI76_RS10370 at 2196.369 kb on + strand, within BWI76_RS10370 at 2196.452 kb on - strand, within BWI76_RS10370 at 2196.630 kb on + strand, within BWI76_RS10370 at 2196.630 kb on + strand, within BWI76_RS10370 at 2196.630 kb on + strand, within BWI76_RS10370 at 2196.638 kb on - strand, within BWI76_RS10370 at 2196.638 kb on - strand, within BWI76_RS10370 at 2196.638 kb on - strand, within BWI76_RS10370 at 2196.844 kb on - strand at 2196.909 kb on + strand at 2196.924 kb on + strand, within BWI76_RS10375
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB31 MOI 1 remove 2,194,443 + BWI76_RS10360 0.65 -1.3 2,194,673 - -0.4 2,194,742 + +0.1 2,194,883 + -0.0 2,194,967 + -2.4 2,195,026 + +0.4 2,195,139 - -1.3 2,195,193 + BWI76_RS10365 0.12 +0.9 2,195,357 - BWI76_RS10365 0.29 -0.1 2,195,586 + BWI76_RS10365 0.52 +1.5 2,195,586 + BWI76_RS10365 0.52 +1.6 2,195,586 + BWI76_RS10365 0.52 -0.6 2,196,102 - -2.2 2,196,210 + BWI76_RS10370 0.19 -0.9 2,196,293 - BWI76_RS10370 0.30 -0.3 2,196,369 + BWI76_RS10370 0.39 -0.3 2,196,452 - BWI76_RS10370 0.50 -1.4 2,196,630 + BWI76_RS10370 0.73 +0.2 2,196,630 + BWI76_RS10370 0.73 -0.5 2,196,630 + BWI76_RS10370 0.73 +0.2 2,196,638 - BWI76_RS10370 0.74 +4.6 2,196,638 - BWI76_RS10370 0.74 -0.2 2,196,638 - BWI76_RS10370 0.74 -0.0 2,196,844 - -0.0 2,196,909 + +0.4 2,196,924 + BWI76_RS10375 0.10 +0.3
Or see this region's nucleotide sequence