Experiment: KLEB31 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10245 and BWI76_RS10250 are separated by 68 nucleotides BWI76_RS10250 and BWI76_RS10255 overlap by 17 nucleotides BWI76_RS10255 and BWI76_RS10260 are separated by 10 nucleotides
BWI76_RS10245: BWI76_RS10245 - aspartate aminotransferase, at 2,169,600 to 2,170,808
_RS10245
BWI76_RS10250: BWI76_RS10250 - glutamine amidotransferase, at 2,170,877 to 2,171,974
_RS10250
BWI76_RS10255: BWI76_RS10255 - dihydrodipicolinate synthase family protein, at 2,171,958 to 2,172,881
_RS10255
BWI76_RS10260: BWI76_RS10260 - creatinase, at 2,172,892 to 2,174,061
_RS10260
Position (kb)
2170
2171
2172 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2170.526 kb on + strand, within BWI76_RS10245 at 2170.729 kb on + strand at 2171.157 kb on - strand, within BWI76_RS10250 at 2171.184 kb on - strand, within BWI76_RS10250 at 2171.448 kb on + strand, within BWI76_RS10250 at 2171.456 kb on - strand, within BWI76_RS10250 at 2171.456 kb on - strand, within BWI76_RS10250 at 2171.552 kb on + strand, within BWI76_RS10250 at 2171.900 kb on - strand at 2172.023 kb on - strand at 2172.023 kb on - strand at 2172.023 kb on - strand at 2172.668 kb on - strand, within BWI76_RS10255 at 2172.750 kb on + strand, within BWI76_RS10255 at 2172.750 kb on + strand, within BWI76_RS10255
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB31 MOI 1 remove 2,170,526 + BWI76_RS10245 0.77 +0.7 2,170,729 + -0.1 2,171,157 - BWI76_RS10250 0.26 +0.8 2,171,184 - BWI76_RS10250 0.28 -1.0 2,171,448 + BWI76_RS10250 0.52 -2.5 2,171,456 - BWI76_RS10250 0.53 +0.2 2,171,456 - BWI76_RS10250 0.53 -0.6 2,171,552 + BWI76_RS10250 0.61 -0.9 2,171,900 - -0.5 2,172,023 - +1.8 2,172,023 - +0.4 2,172,023 - +0.3 2,172,668 - BWI76_RS10255 0.77 -3.8 2,172,750 + BWI76_RS10255 0.86 -0.3 2,172,750 + BWI76_RS10255 0.86 +0.5
Or see this region's nucleotide sequence