Experiment: KLEB31 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS09675 and BWI76_RS09680 are separated by 200 nucleotides BWI76_RS09680 and BWI76_RS09685 are separated by 10 nucleotides BWI76_RS09685 and BWI76_RS09690 are separated by 1 nucleotides
BWI76_RS09675: BWI76_RS09675 - serine--tRNA ligase, at 2,027,200 to 2,028,492
_RS09675
BWI76_RS09680: BWI76_RS09680 - dimethyl sulfoxide reductase subunit A, at 2,028,693 to 2,031,131
_RS09680
BWI76_RS09685: BWI76_RS09685 - dimethylsulfoxide reductase, chain B, at 2,031,142 to 2,031,759
_RS09685
BWI76_RS09690: BWI76_RS09690 - dimethyl sulfoxide reductase, at 2,031,761 to 2,032,624
_RS09690
Position (kb)
2028
2029
2030
2031
2032 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2028.723 kb on - strand at 2028.793 kb on + strand at 2028.798 kb on + strand at 2028.844 kb on - strand at 2028.884 kb on + strand at 2028.975 kb on + strand, within BWI76_RS09680 at 2029.726 kb on - strand, within BWI76_RS09680 at 2029.749 kb on - strand, within BWI76_RS09680 at 2029.955 kb on + strand, within BWI76_RS09680 at 2030.539 kb on + strand, within BWI76_RS09680 at 2030.994 kb on - strand at 2030.998 kb on - strand at 2030.998 kb on - strand at 2031.012 kb on - strand at 2031.140 kb on - strand at 2031.752 kb on - strand at 2031.861 kb on - strand, within BWI76_RS09690
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB31 MOI 1 remove 2,028,723 - +0.5 2,028,793 + +0.6 2,028,798 + +0.9 2,028,844 - +0.0 2,028,884 + +0.9 2,028,975 + BWI76_RS09680 0.12 +3.4 2,029,726 - BWI76_RS09680 0.42 +0.6 2,029,749 - BWI76_RS09680 0.43 -0.3 2,029,955 + BWI76_RS09680 0.52 +1.9 2,030,539 + BWI76_RS09680 0.76 +1.3 2,030,994 - +3.0 2,030,998 - -0.4 2,030,998 - +0.3 2,031,012 - +1.0 2,031,140 - +0.6 2,031,752 - -0.0 2,031,861 - BWI76_RS09690 0.12 -0.3
Or see this region's nucleotide sequence