Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS09680

Experiment: KLEB31 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS09675 and BWI76_RS09680 are separated by 200 nucleotidesBWI76_RS09680 and BWI76_RS09685 are separated by 10 nucleotidesBWI76_RS09685 and BWI76_RS09690 are separated by 1 nucleotides BWI76_RS09675: BWI76_RS09675 - serine--tRNA ligase, at 2,027,200 to 2,028,492 _RS09675 BWI76_RS09680: BWI76_RS09680 - dimethyl sulfoxide reductase subunit A, at 2,028,693 to 2,031,131 _RS09680 BWI76_RS09685: BWI76_RS09685 - dimethylsulfoxide reductase, chain B, at 2,031,142 to 2,031,759 _RS09685 BWI76_RS09690: BWI76_RS09690 - dimethyl sulfoxide reductase, at 2,031,761 to 2,032,624 _RS09690 Position (kb) 2028 2029 2030 2031 2032Strain fitness (log2 ratio) -1 0 1 2 3at 2028.723 kb on - strandat 2028.793 kb on + strandat 2028.798 kb on + strandat 2028.844 kb on - strandat 2028.884 kb on + strandat 2028.975 kb on + strand, within BWI76_RS09680at 2029.726 kb on - strand, within BWI76_RS09680at 2029.749 kb on - strand, within BWI76_RS09680at 2029.955 kb on + strand, within BWI76_RS09680at 2030.539 kb on + strand, within BWI76_RS09680at 2030.994 kb on - strandat 2030.998 kb on - strandat 2030.998 kb on - strandat 2031.012 kb on - strandat 2031.140 kb on - strandat 2031.752 kb on - strandat 2031.861 kb on - strand, within BWI76_RS09690

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB31 MOI 1
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2,028,723 - +0.5
2,028,793 + +0.6
2,028,798 + +0.9
2,028,844 - +0.0
2,028,884 + +0.9
2,028,975 + BWI76_RS09680 0.12 +3.4
2,029,726 - BWI76_RS09680 0.42 +0.6
2,029,749 - BWI76_RS09680 0.43 -0.3
2,029,955 + BWI76_RS09680 0.52 +1.9
2,030,539 + BWI76_RS09680 0.76 +1.3
2,030,994 - +3.0
2,030,998 - -0.4
2,030,998 - +0.3
2,031,012 - +1.0
2,031,140 - +0.6
2,031,752 - -0.0
2,031,861 - BWI76_RS09690 0.12 -0.3

Or see this region's nucleotide sequence