Experiment: KLEB31 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS09240 and BWI76_RS09245 overlap by 4 nucleotides BWI76_RS09245 and BWI76_RS09250 are separated by 114 nucleotides BWI76_RS09250 and BWI76_RS09255 are separated by 398 nucleotides
BWI76_RS09240: BWI76_RS09240 - phosphoadenosine phosphosulfate reductase, at 1,945,101 to 1,946,111
_RS09240
BWI76_RS09245: BWI76_RS09245 - bacteriophage replication gene A protein, at 1,946,108 to 1,948,519
_RS09245
BWI76_RS09250: BWI76_RS09250 - hypothetical protein, at 1,948,634 to 1,948,819
_RS09250
BWI76_RS09255: BWI76_RS09255 - hypothetical protein, at 1,949,218 to 1,950,090
_RS09255
Position (kb)
1946
1947
1948
1949 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1945.182 kb on - strand at 1945.182 kb on - strand at 1945.218 kb on - strand, within BWI76_RS09240 at 1945.331 kb on + strand, within BWI76_RS09240 at 1945.361 kb on + strand at 1945.535 kb on + strand, within BWI76_RS09240 at 1945.606 kb on + strand, within BWI76_RS09240 at 1946.216 kb on + strand at 1946.235 kb on + strand at 1946.370 kb on + strand, within BWI76_RS09245 at 1946.378 kb on - strand, within BWI76_RS09245 at 1946.440 kb on + strand, within BWI76_RS09245 at 1946.467 kb on - strand, within BWI76_RS09245 at 1946.586 kb on + strand, within BWI76_RS09245 at 1946.594 kb on - strand, within BWI76_RS09245 at 1946.604 kb on - strand, within BWI76_RS09245 at 1946.657 kb on + strand, within BWI76_RS09245 at 1946.701 kb on + strand, within BWI76_RS09245 at 1946.985 kb on + strand, within BWI76_RS09245 at 1947.111 kb on + strand, within BWI76_RS09245 at 1947.129 kb on - strand, within BWI76_RS09245 at 1947.270 kb on - strand, within BWI76_RS09245 at 1947.405 kb on + strand, within BWI76_RS09245 at 1947.420 kb on + strand, within BWI76_RS09245 at 1947.428 kb on - strand, within BWI76_RS09245 at 1947.521 kb on - strand, within BWI76_RS09245 at 1947.549 kb on + strand, within BWI76_RS09245 at 1947.567 kb on + strand, within BWI76_RS09245 at 1947.595 kb on + strand, within BWI76_RS09245 at 1947.660 kb on + strand, within BWI76_RS09245 at 1947.668 kb on - strand at 1947.831 kb on - strand, within BWI76_RS09245 at 1947.935 kb on + strand, within BWI76_RS09245 at 1947.937 kb on + strand, within BWI76_RS09245 at 1948.149 kb on - strand, within BWI76_RS09245 at 1948.162 kb on - strand, within BWI76_RS09245 at 1948.671 kb on + strand, within BWI76_RS09250 at 1948.714 kb on - strand, within BWI76_RS09250 at 1948.776 kb on + strand, within BWI76_RS09250 at 1948.776 kb on + strand, within BWI76_RS09250 at 1948.776 kb on + strand, within BWI76_RS09250 at 1948.789 kb on - strand, within BWI76_RS09250 at 1948.791 kb on + strand, within BWI76_RS09250 at 1948.796 kb on + strand, within BWI76_RS09250 at 1948.799 kb on - strand, within BWI76_RS09250 at 1948.842 kb on + strand at 1948.877 kb on + strand at 1948.927 kb on + strand at 1948.964 kb on + strand at 1948.971 kb on - strand at 1949.002 kb on + strand at 1949.035 kb on - strand at 1949.039 kb on - strand at 1949.039 kb on - strand at 1949.039 kb on - strand at 1949.093 kb on - strand at 1949.098 kb on - strand at 1949.233 kb on + strand at 1949.243 kb on + strand at 1949.243 kb on + strand at 1949.244 kb on + strand at 1949.251 kb on + strand at 1949.337 kb on + strand, within BWI76_RS09255 at 1949.337 kb on + strand, within BWI76_RS09255 at 1949.512 kb on + strand, within BWI76_RS09255
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB31 MOI 1 remove 1,945,182 - -1.1 1,945,182 - -2.1 1,945,218 - BWI76_RS09240 0.12 +0.7 1,945,331 + BWI76_RS09240 0.23 +1.5 1,945,361 + +1.1 1,945,535 + BWI76_RS09240 0.43 +0.4 1,945,606 + BWI76_RS09240 0.50 -0.6 1,946,216 + +2.1 1,946,235 + -1.3 1,946,370 + BWI76_RS09245 0.11 -1.8 1,946,378 - BWI76_RS09245 0.11 +1.1 1,946,440 + BWI76_RS09245 0.14 +2.1 1,946,467 - BWI76_RS09245 0.15 -0.1 1,946,586 + BWI76_RS09245 0.20 +1.1 1,946,594 - BWI76_RS09245 0.20 -0.2 1,946,604 - BWI76_RS09245 0.21 -0.4 1,946,657 + BWI76_RS09245 0.23 +1.7 1,946,701 + BWI76_RS09245 0.25 +2.7 1,946,985 + BWI76_RS09245 0.36 -2.1 1,947,111 + BWI76_RS09245 0.42 +0.1 1,947,129 - BWI76_RS09245 0.42 -0.6 1,947,270 - BWI76_RS09245 0.48 +0.6 1,947,405 + BWI76_RS09245 0.54 +0.9 1,947,420 + BWI76_RS09245 0.54 -1.2 1,947,428 - BWI76_RS09245 0.55 +1.0 1,947,521 - BWI76_RS09245 0.59 +1.1 1,947,549 + BWI76_RS09245 0.60 +0.1 1,947,567 + BWI76_RS09245 0.60 -0.3 1,947,595 + BWI76_RS09245 0.62 +0.2 1,947,660 + BWI76_RS09245 0.64 +1.2 1,947,668 - -0.5 1,947,831 - BWI76_RS09245 0.71 +0.2 1,947,935 + BWI76_RS09245 0.76 +0.3 1,947,937 + BWI76_RS09245 0.76 +0.6 1,948,149 - BWI76_RS09245 0.85 -0.6 1,948,162 - BWI76_RS09245 0.85 -0.4 1,948,671 + BWI76_RS09250 0.20 +1.4 1,948,714 - BWI76_RS09250 0.43 +1.9 1,948,776 + BWI76_RS09250 0.76 +0.4 1,948,776 + BWI76_RS09250 0.76 -1.8 1,948,776 + BWI76_RS09250 0.76 +1.4 1,948,789 - BWI76_RS09250 0.83 +1.1 1,948,791 + BWI76_RS09250 0.84 -0.9 1,948,796 + BWI76_RS09250 0.87 -0.8 1,948,799 - BWI76_RS09250 0.89 +0.7 1,948,842 + +0.2 1,948,877 + -1.5 1,948,927 + -0.6 1,948,964 + +0.1 1,948,971 - +1.5 1,949,002 + +0.9 1,949,035 - +0.7 1,949,039 - +2.4 1,949,039 - +0.8 1,949,039 - +2.2 1,949,093 - +0.6 1,949,098 - +2.6 1,949,233 + +1.6 1,949,243 + -1.9 1,949,243 + +0.1 1,949,244 + +0.8 1,949,251 + -0.7 1,949,337 + BWI76_RS09255 0.14 -1.9 1,949,337 + BWI76_RS09255 0.14 +1.1 1,949,512 + BWI76_RS09255 0.34 +0.1
Or see this region's nucleotide sequence