Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS09060

Experiment: KLEB31 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS09055 and BWI76_RS09060 overlap by 4 nucleotidesBWI76_RS09060 and BWI76_RS09065 are separated by 136 nucleotides BWI76_RS09055: BWI76_RS09055 - LacI family transcriptional regulator, at 1,910,109 to 1,911,182 _RS09055 BWI76_RS09060: BWI76_RS09060 - hypothetical protein, at 1,911,179 to 1,912,444 _RS09060 BWI76_RS09065: BWI76_RS09065 - sugar phosphatase SupH, at 1,912,581 to 1,913,396 _RS09065 Position (kb) 1911 1912 1913Strain fitness (log2 ratio) -1 0 1at 1910.223 kb on + strand, within BWI76_RS09055at 1910.231 kb on - strand, within BWI76_RS09055at 1910.397 kb on - strand, within BWI76_RS09055at 1910.418 kb on - strand, within BWI76_RS09055at 1910.463 kb on + strand, within BWI76_RS09055at 1910.725 kb on - strand, within BWI76_RS09055at 1911.383 kb on - strand, within BWI76_RS09060at 1911.667 kb on - strand, within BWI76_RS09060at 1911.710 kb on - strand, within BWI76_RS09060at 1912.334 kb on + strandat 1912.435 kb on - strandat 1912.570 kb on - strandat 1912.791 kb on - strand, within BWI76_RS09065at 1912.817 kb on - strand, within BWI76_RS09065at 1912.827 kb on - strand, within BWI76_RS09065at 1913.000 kb on + strand, within BWI76_RS09065

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB31 MOI 1
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1,910,223 + BWI76_RS09055 0.11 +0.9
1,910,231 - BWI76_RS09055 0.11 +0.1
1,910,397 - BWI76_RS09055 0.27 +1.1
1,910,418 - BWI76_RS09055 0.29 +0.0
1,910,463 + BWI76_RS09055 0.33 -0.4
1,910,725 - BWI76_RS09055 0.57 -0.8
1,911,383 - BWI76_RS09060 0.16 -1.3
1,911,667 - BWI76_RS09060 0.39 -0.8
1,911,710 - BWI76_RS09060 0.42 +0.2
1,912,334 + +0.0
1,912,435 - +0.2
1,912,570 - -0.4
1,912,791 - BWI76_RS09065 0.26 -0.6
1,912,817 - BWI76_RS09065 0.29 -0.3
1,912,827 - BWI76_RS09065 0.30 +0.5
1,913,000 + BWI76_RS09065 0.51 -1.0

Or see this region's nucleotide sequence