Experiment: KLEB31 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS08980 and BWI76_RS08985 are separated by 221 nucleotides BWI76_RS08985 and BWI76_RS08990 are separated by 351 nucleotides BWI76_RS08990 and BWI76_RS08995 are separated by 69 nucleotides
BWI76_RS08980: BWI76_RS08980 - DNA starvation/stationary phase protection protein, at 1,892,519 to 1,893,022
_RS08980
BWI76_RS08985: BWI76_RS08985 - threonine/homoserine exporter RhtA, at 1,893,244 to 1,894,131
_RS08985
BWI76_RS08990: BWI76_RS08990 - outer membrane protein X, at 1,894,483 to 1,894,995
_RS08990
BWI76_RS08995: BWI76_RS08995 - sulfatase, at 1,895,065 to 1,896,642
_RS08995
Position (kb)
1893
1894
1895 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1892.449 kb on + strand at 1892.457 kb on - strand at 1892.524 kb on - strand at 1892.683 kb on - strand, within BWI76_RS08980 at 1893.108 kb on + strand at 1893.299 kb on - strand at 1893.461 kb on + strand, within BWI76_RS08985 at 1893.469 kb on - strand, within BWI76_RS08985 at 1893.586 kb on + strand, within BWI76_RS08985 at 1893.950 kb on + strand, within BWI76_RS08985 at 1894.194 kb on - strand at 1894.288 kb on - strand at 1894.372 kb on + strand at 1894.441 kb on + strand at 1894.453 kb on + strand at 1894.507 kb on + strand at 1894.616 kb on - strand, within BWI76_RS08990 at 1894.975 kb on + strand at 1895.102 kb on - strand at 1895.112 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB31 MOI 1 remove 1,892,449 + -0.8 1,892,457 - +0.4 1,892,524 - +1.1 1,892,683 - BWI76_RS08980 0.33 +0.4 1,893,108 + -0.8 1,893,299 - -0.2 1,893,461 + BWI76_RS08985 0.24 +1.7 1,893,469 - BWI76_RS08985 0.25 -1.5 1,893,586 + BWI76_RS08985 0.39 +1.5 1,893,950 + BWI76_RS08985 0.80 +3.1 1,894,194 - -0.4 1,894,288 - +1.3 1,894,372 + +0.1 1,894,441 + +0.0 1,894,453 + -0.7 1,894,507 + +1.0 1,894,616 - BWI76_RS08990 0.26 +1.1 1,894,975 + -0.1 1,895,102 - +0.2 1,895,112 - +0.6
Or see this region's nucleotide sequence