Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS08855

Experiment: KLEB31 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS08850 and BWI76_RS08855 overlap by 4 nucleotidesBWI76_RS08855 and BWI76_RS08860 are separated by 143 nucleotidesBWI76_RS08860 and BWI76_RS08865 overlap by 39 nucleotides BWI76_RS08850: BWI76_RS08850 - cardiolipin synthase B, at 1,863,127 to 1,864,371 _RS08850 BWI76_RS08855: BWI76_RS08855 - endonuclease/exonuclease/phosphatase, at 1,864,368 to 1,865,117 _RS08855 BWI76_RS08860: BWI76_RS08860 - hypothetical protein, at 1,865,261 to 1,865,671 _RS08860 BWI76_RS08865: BWI76_RS08865 - ABC transporter inner membrane, at 1,865,633 to 1,866,739 _RS08865 Position (kb) 1864 1865 1866Strain fitness (log2 ratio) -1 0 1 2at 1863.499 kb on - strand, within BWI76_RS08850at 1864.152 kb on + strand, within BWI76_RS08850at 1864.207 kb on - strand, within BWI76_RS08850at 1864.490 kb on - strand, within BWI76_RS08855at 1864.572 kb on - strand, within BWI76_RS08855at 1864.634 kb on + strand, within BWI76_RS08855at 1865.569 kb on - strand, within BWI76_RS08860

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB31 MOI 1
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1,863,499 - BWI76_RS08850 0.30 +0.4
1,864,152 + BWI76_RS08850 0.82 +1.0
1,864,207 - BWI76_RS08850 0.87 +0.6
1,864,490 - BWI76_RS08855 0.16 +0.4
1,864,572 - BWI76_RS08855 0.27 +0.5
1,864,634 + BWI76_RS08855 0.35 +2.2
1,865,569 - BWI76_RS08860 0.75 -0.4

Or see this region's nucleotide sequence