Experiment: KLEB31 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS08765 and BWI76_RS08770 overlap by 17 nucleotides BWI76_RS08770 and BWI76_RS08775 overlap by 8 nucleotides BWI76_RS08775 and BWI76_RS08780 overlap by 59 nucleotides
BWI76_RS08765: BWI76_RS08765 - 8-amino-7-oxononanoate synthase, at 1,847,450 to 1,848,607
_RS08765
BWI76_RS08770: BWI76_RS08770 - malonyl-ACP O-methyltransferase BioC, at 1,848,591 to 1,849,346
_RS08770
BWI76_RS08775: BWI76_RS08775 - dethiobiotin synthase, at 1,849,339 to 1,850,061
_RS08775
BWI76_RS08780: BWI76_RS08780 - ABC transporter, at 1,850,003 to 1,850,725
_RS08780
Position (kb) 1849
1850
1851 Strain fitness (log2 ratio) -1
0
1
2 at 1848.463 kb on + strand, within BWI76_RS08765 at 1848.539 kb on - strand at 1848.691 kb on + strand, within BWI76_RS08770 at 1848.740 kb on + strand, within BWI76_RS08770 at 1848.968 kb on + strand, within BWI76_RS08770 at 1849.437 kb on - strand, within BWI76_RS08775 at 1849.551 kb on + strand, within BWI76_RS08775 at 1849.559 kb on - strand, within BWI76_RS08775 at 1849.794 kb on + strand, within BWI76_RS08775 at 1849.817 kb on - strand, within BWI76_RS08775 at 1850.085 kb on + strand, within BWI76_RS08780 at 1850.272 kb on - strand, within BWI76_RS08780 at 1850.583 kb on + strand, within BWI76_RS08780 at 1850.997 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB31 MOI 1 remove 1,848,463 + BWI76_RS08765 0.87 +1.7 1,848,539 - -0.9 1,848,691 + BWI76_RS08770 0.13 -1.4 1,848,740 + BWI76_RS08770 0.20 +0.2 1,848,968 + BWI76_RS08770 0.50 -0.6 1,849,437 - BWI76_RS08775 0.14 +0.6 1,849,551 + BWI76_RS08775 0.29 +0.6 1,849,559 - BWI76_RS08775 0.30 -1.6 1,849,794 + BWI76_RS08775 0.63 -0.0 1,849,817 - BWI76_RS08775 0.66 -0.2 1,850,085 + BWI76_RS08780 0.11 -0.4 1,850,272 - BWI76_RS08780 0.37 +2.0 1,850,583 + BWI76_RS08780 0.80 +0.8 1,850,997 - +2.5
Or see this region's nucleotide sequence