Experiment: KLEB31 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS07335 and BWI76_RS07340 are separated by 430 nucleotides BWI76_RS07340 and BWI76_RS07345 are separated by 19 nucleotides BWI76_RS07345 and BWI76_RS07350 are separated by 131 nucleotides
BWI76_RS07335: BWI76_RS07335 - hypothetical protein, at 1,545,654 to 1,546,124
_RS07335
BWI76_RS07340: BWI76_RS07340 - amidohydrolase, at 1,546,555 to 1,547,733
_RS07340
BWI76_RS07345: BWI76_RS07345 - family 3 extracellular solute-binding protein, at 1,547,753 to 1,548,529
_RS07345
BWI76_RS07350: BWI76_RS07350 - alcohol dehydrogenase, at 1,548,661 to 1,549,614
_RS07350
Position (kb)
1546
1547
1548 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1545.571 kb on + strand at 1545.579 kb on - strand at 1545.589 kb on - strand at 1545.603 kb on + strand at 1545.611 kb on - strand at 1545.616 kb on + strand at 1545.647 kb on - strand at 1545.686 kb on + strand at 1545.701 kb on - strand, within BWI76_RS07335 at 1545.701 kb on - strand, within BWI76_RS07335 at 1545.707 kb on + strand, within BWI76_RS07335 at 1545.707 kb on + strand, within BWI76_RS07335 at 1545.707 kb on + strand, within BWI76_RS07335 at 1545.707 kb on + strand, within BWI76_RS07335 at 1545.709 kb on + strand, within BWI76_RS07335 at 1545.715 kb on - strand, within BWI76_RS07335 at 1545.715 kb on - strand, within BWI76_RS07335 at 1545.715 kb on - strand, within BWI76_RS07335 at 1545.721 kb on + strand, within BWI76_RS07335 at 1545.721 kb on + strand, within BWI76_RS07335 at 1545.721 kb on + strand, within BWI76_RS07335 at 1545.726 kb on + strand, within BWI76_RS07335 at 1545.729 kb on - strand, within BWI76_RS07335 at 1545.729 kb on - strand, within BWI76_RS07335 at 1545.729 kb on - strand, within BWI76_RS07335 at 1545.729 kb on - strand, within BWI76_RS07335 at 1545.729 kb on - strand, within BWI76_RS07335 at 1545.742 kb on + strand, within BWI76_RS07335 at 1545.746 kb on + strand, within BWI76_RS07335 at 1545.750 kb on - strand, within BWI76_RS07335 at 1545.750 kb on - strand, within BWI76_RS07335 at 1545.752 kb on + strand, within BWI76_RS07335 at 1545.759 kb on - strand, within BWI76_RS07335 at 1545.759 kb on - strand, within BWI76_RS07335 at 1545.760 kb on - strand, within BWI76_RS07335 at 1545.812 kb on + strand, within BWI76_RS07335 at 1545.812 kb on + strand, within BWI76_RS07335 at 1545.875 kb on + strand, within BWI76_RS07335 at 1545.877 kb on - strand, within BWI76_RS07335 at 1545.881 kb on + strand, within BWI76_RS07335 at 1545.893 kb on - strand, within BWI76_RS07335 at 1545.903 kb on - strand, within BWI76_RS07335 at 1545.913 kb on - strand, within BWI76_RS07335 at 1546.031 kb on + strand, within BWI76_RS07335 at 1546.039 kb on - strand, within BWI76_RS07335 at 1546.044 kb on + strand, within BWI76_RS07335 at 1546.100 kb on + strand at 1546.100 kb on + strand at 1546.100 kb on + strand at 1546.108 kb on - strand at 1546.108 kb on - strand at 1546.134 kb on + strand at 1546.142 kb on - strand at 1546.156 kb on + strand at 1546.157 kb on + strand at 1546.164 kb on - strand at 1546.165 kb on - strand at 1546.202 kb on + strand at 1546.203 kb on + strand at 1546.210 kb on - strand at 1546.217 kb on + strand at 1546.217 kb on + strand at 1546.223 kb on - strand at 1546.223 kb on - strand at 1546.225 kb on - strand at 1546.227 kb on + strand at 1546.227 kb on + strand at 1546.227 kb on + strand at 1546.227 kb on + strand at 1546.227 kb on + strand at 1546.227 kb on + strand at 1546.227 kb on + strand at 1546.227 kb on + strand at 1546.227 kb on + strand at 1546.227 kb on + strand at 1546.227 kb on + strand at 1546.227 kb on + strand at 1546.227 kb on + strand at 1546.227 kb on + strand at 1546.227 kb on + strand at 1546.227 kb on + strand at 1546.227 kb on + strand at 1546.227 kb on + strand at 1546.227 kb on + strand at 1546.227 kb on + strand at 1546.227 kb on + strand at 1546.227 kb on + strand at 1546.230 kb on - strand at 1546.230 kb on - strand at 1546.230 kb on - strand at 1546.230 kb on - strand at 1546.230 kb on - strand at 1546.235 kb on - strand at 1546.235 kb on - strand at 1546.235 kb on - strand at 1546.235 kb on - strand at 1546.235 kb on - strand at 1546.235 kb on - strand at 1546.235 kb on - strand at 1546.235 kb on - strand at 1546.235 kb on - strand at 1546.235 kb on - strand at 1546.235 kb on - strand at 1546.235 kb on - strand at 1546.235 kb on - strand at 1546.235 kb on - strand at 1546.235 kb on - strand at 1546.235 kb on - strand at 1546.235 kb on - strand at 1546.235 kb on - strand at 1546.235 kb on - strand at 1546.235 kb on - strand at 1546.235 kb on - strand at 1546.235 kb on - strand at 1546.235 kb on - strand at 1546.235 kb on - strand at 1546.235 kb on - strand at 1546.235 kb on - strand at 1546.235 kb on - strand at 1546.237 kb on + strand at 1546.240 kb on + strand at 1546.240 kb on + strand at 1546.245 kb on + strand at 1546.256 kb on + strand at 1546.257 kb on - strand at 1546.261 kb on + strand at 1546.264 kb on - strand at 1546.269 kb on - strand at 1546.291 kb on + strand at 1546.298 kb on - strand at 1546.309 kb on + strand at 1546.309 kb on + strand at 1546.316 kb on + strand at 1546.337 kb on - strand at 1546.354 kb on - strand at 1546.396 kb on - strand at 1546.398 kb on + strand at 1546.399 kb on + strand at 1546.399 kb on + strand at 1546.399 kb on + strand at 1546.399 kb on + strand at 1546.399 kb on + strand at 1546.399 kb on + strand at 1546.401 kb on - strand at 1546.401 kb on - strand at 1546.407 kb on - strand at 1546.407 kb on - strand at 1546.407 kb on - strand at 1546.407 kb on - strand at 1546.407 kb on - strand at 1546.409 kb on + strand at 1546.409 kb on + strand at 1546.409 kb on + strand at 1546.412 kb on - strand at 1546.417 kb on - strand at 1546.417 kb on - strand at 1546.417 kb on - strand at 1546.418 kb on - strand at 1546.422 kb on - strand at 1546.445 kb on + strand at 1546.490 kb on + strand at 1546.491 kb on + strand at 1546.491 kb on + strand at 1546.498 kb on + strand at 1546.500 kb on - strand at 1546.515 kb on + strand at 1546.542 kb on + strand at 1546.555 kb on + strand at 1546.557 kb on + strand at 1546.559 kb on - strand at 1546.586 kb on - strand at 1546.602 kb on - strand at 1546.651 kb on - strand at 1546.653 kb on + strand at 1546.653 kb on + strand at 1546.653 kb on + strand at 1546.653 kb on + strand at 1546.653 kb on + strand at 1546.653 kb on + strand at 1546.664 kb on + strand at 1546.676 kb on + strand, within BWI76_RS07340 at 1546.676 kb on + strand, within BWI76_RS07340 at 1546.676 kb on + strand, within BWI76_RS07340 at 1546.676 kb on + strand, within BWI76_RS07340 at 1546.684 kb on - strand, within BWI76_RS07340 at 1546.684 kb on - strand, within BWI76_RS07340 at 1546.695 kb on + strand, within BWI76_RS07340 at 1546.695 kb on + strand, within BWI76_RS07340 at 1546.695 kb on + strand at 1546.697 kb on + strand, within BWI76_RS07340 at 1546.697 kb on + strand, within BWI76_RS07340 at 1546.697 kb on + strand, within BWI76_RS07340 at 1546.697 kb on + strand, within BWI76_RS07340 at 1546.697 kb on + strand, within BWI76_RS07340 at 1546.697 kb on + strand, within BWI76_RS07340 at 1546.698 kb on - strand, within BWI76_RS07340 at 1546.698 kb on - strand, within BWI76_RS07340 at 1546.702 kb on + strand, within BWI76_RS07340 at 1546.702 kb on + strand, within BWI76_RS07340 at 1546.702 kb on + strand at 1546.702 kb on + strand, within BWI76_RS07340 at 1546.703 kb on - strand, within BWI76_RS07340 at 1546.703 kb on - strand, within BWI76_RS07340 at 1546.703 kb on - strand, within BWI76_RS07340 at 1546.705 kb on - strand, within BWI76_RS07340 at 1546.705 kb on - strand, within BWI76_RS07340 at 1546.705 kb on - strand, within BWI76_RS07340 at 1546.705 kb on - strand, within BWI76_RS07340 at 1546.709 kb on + strand, within BWI76_RS07340 at 1546.734 kb on + strand, within BWI76_RS07340 at 1546.735 kb on + strand, within BWI76_RS07340 at 1546.742 kb on + strand, within BWI76_RS07340 at 1546.742 kb on - strand, within BWI76_RS07340 at 1546.743 kb on - strand, within BWI76_RS07340 at 1546.777 kb on - strand, within BWI76_RS07340 at 1546.813 kb on + strand, within BWI76_RS07340 at 1546.824 kb on + strand, within BWI76_RS07340 at 1546.881 kb on - strand, within BWI76_RS07340 at 1546.929 kb on + strand, within BWI76_RS07340 at 1546.944 kb on + strand, within BWI76_RS07340 at 1546.947 kb on + strand, within BWI76_RS07340 at 1546.955 kb on - strand, within BWI76_RS07340 at 1546.972 kb on + strand, within BWI76_RS07340 at 1547.036 kb on + strand, within BWI76_RS07340 at 1547.228 kb on + strand, within BWI76_RS07340 at 1547.324 kb on + strand, within BWI76_RS07340 at 1547.644 kb on + strand at 1547.732 kb on - strand at 1547.734 kb on + strand at 1547.778 kb on - strand at 1547.778 kb on - strand at 1547.788 kb on - strand at 1547.855 kb on - strand, within BWI76_RS07345 at 1547.884 kb on + strand, within BWI76_RS07345 at 1547.929 kb on + strand, within BWI76_RS07345 at 1547.937 kb on - strand, within BWI76_RS07345 at 1547.943 kb on - strand, within BWI76_RS07345 at 1547.970 kb on + strand, within BWI76_RS07345 at 1548.030 kb on - strand, within BWI76_RS07345 at 1548.030 kb on - strand, within BWI76_RS07345 at 1548.067 kb on + strand, within BWI76_RS07345 at 1548.075 kb on - strand, within BWI76_RS07345 at 1548.075 kb on - strand, within BWI76_RS07345 at 1548.188 kb on + strand, within BWI76_RS07345 at 1548.201 kb on - strand, within BWI76_RS07345 at 1548.539 kb on + strand at 1548.553 kb on + strand at 1548.553 kb on + strand at 1548.561 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB31 MOI 1 remove 1,545,571 + -0.1 1,545,579 - -0.6 1,545,589 - -0.5 1,545,603 + +0.3 1,545,611 - +0.3 1,545,616 + +1.1 1,545,647 - +1.3 1,545,686 + -0.4 1,545,701 - BWI76_RS07335 0.10 +0.2 1,545,701 - BWI76_RS07335 0.10 +0.6 1,545,707 + BWI76_RS07335 0.11 +0.1 1,545,707 + BWI76_RS07335 0.11 -0.8 1,545,707 + BWI76_RS07335 0.11 -0.9 1,545,707 + BWI76_RS07335 0.11 +0.5 1,545,709 + BWI76_RS07335 0.12 +0.5 1,545,715 - BWI76_RS07335 0.13 -0.4 1,545,715 - BWI76_RS07335 0.13 -0.0 1,545,715 - BWI76_RS07335 0.13 -0.3 1,545,721 + BWI76_RS07335 0.14 -0.4 1,545,721 + BWI76_RS07335 0.14 +0.7 1,545,721 + BWI76_RS07335 0.14 -2.1 1,545,726 + BWI76_RS07335 0.15 -0.8 1,545,729 - BWI76_RS07335 0.16 +0.8 1,545,729 - BWI76_RS07335 0.16 +0.0 1,545,729 - BWI76_RS07335 0.16 -2.3 1,545,729 - BWI76_RS07335 0.16 +0.1 1,545,729 - BWI76_RS07335 0.16 -0.9 1,545,742 + BWI76_RS07335 0.19 +0.7 1,545,746 + BWI76_RS07335 0.20 +0.7 1,545,750 - BWI76_RS07335 0.20 -1.5 1,545,750 - BWI76_RS07335 0.20 +0.9 1,545,752 + BWI76_RS07335 0.21 -0.4 1,545,759 - BWI76_RS07335 0.22 -0.1 1,545,759 - BWI76_RS07335 0.22 -0.4 1,545,760 - BWI76_RS07335 0.23 -1.2 1,545,812 + BWI76_RS07335 0.34 +2.1 1,545,812 + BWI76_RS07335 0.34 -2.1 1,545,875 + BWI76_RS07335 0.47 -0.3 1,545,877 - BWI76_RS07335 0.47 +2.6 1,545,881 + BWI76_RS07335 0.48 +0.2 1,545,893 - BWI76_RS07335 0.51 +0.9 1,545,903 - BWI76_RS07335 0.53 +0.1 1,545,913 - BWI76_RS07335 0.55 +0.4 1,546,031 + BWI76_RS07335 0.80 +0.1 1,546,039 - BWI76_RS07335 0.82 +0.4 1,546,044 + BWI76_RS07335 0.83 -0.2 1,546,100 + +1.4 1,546,100 + -0.4 1,546,100 + +0.2 1,546,108 - -1.0 1,546,108 - +1.1 1,546,134 + -2.2 1,546,142 - -1.4 1,546,156 + +0.2 1,546,157 + -0.5 1,546,164 - +1.4 1,546,165 - -0.1 1,546,202 + -1.1 1,546,203 + -1.8 1,546,210 - -0.8 1,546,217 + -0.4 1,546,217 + +0.5 1,546,223 - +1.6 1,546,223 - +0.3 1,546,225 - -0.6 1,546,227 + +1.1 1,546,227 + +1.4 1,546,227 + +0.4 1,546,227 + -0.0 1,546,227 + +0.6 1,546,227 + -0.3 1,546,227 + -1.3 1,546,227 + +2.4 1,546,227 + -0.9 1,546,227 + +0.7 1,546,227 + -0.2 1,546,227 + -0.9 1,546,227 + +1.7 1,546,227 + +0.8 1,546,227 + +3.2 1,546,227 + -1.5 1,546,227 + -0.3 1,546,227 + +1.9 1,546,227 + +1.1 1,546,227 + -0.5 1,546,227 + +1.1 1,546,227 + +1.5 1,546,230 - -0.0 1,546,230 - -0.3 1,546,230 - -1.3 1,546,230 - +0.8 1,546,230 - +1.9 1,546,235 - -0.6 1,546,235 - +0.6 1,546,235 - +0.4 1,546,235 - +0.6 1,546,235 - -1.1 1,546,235 - +0.8 1,546,235 - -0.9 1,546,235 - +0.6 1,546,235 - +0.0 1,546,235 - +1.3 1,546,235 - +1.8 1,546,235 - -1.1 1,546,235 - -0.3 1,546,235 - +0.2 1,546,235 - +0.1 1,546,235 - -0.6 1,546,235 - +0.6 1,546,235 - -1.4 1,546,235 - +0.1 1,546,235 - -0.6 1,546,235 - -0.4 1,546,235 - -0.2 1,546,235 - -0.1 1,546,235 - -1.6 1,546,235 - -0.3 1,546,235 - -0.5 1,546,235 - +0.3 1,546,237 + +1.1 1,546,240 + -1.6 1,546,240 + -0.5 1,546,245 + +0.5 1,546,256 + +1.7 1,546,257 - +1.1 1,546,261 + -0.1 1,546,264 - -1.2 1,546,269 - -2.5 1,546,291 + -0.9 1,546,298 - +1.5 1,546,309 + +1.6 1,546,309 + +1.2 1,546,316 + -0.9 1,546,337 - -0.4 1,546,354 - -0.1 1,546,396 - -0.5 1,546,398 + -0.7 1,546,399 + -0.7 1,546,399 + -0.5 1,546,399 + -1.5 1,546,399 + +0.1 1,546,399 + -1.7 1,546,399 + +2.3 1,546,401 - +0.6 1,546,401 - +2.5 1,546,407 - -0.1 1,546,407 - +0.8 1,546,407 - -0.3 1,546,407 - -1.7 1,546,407 - +0.9 1,546,409 + +0.2 1,546,409 + -1.6 1,546,409 + +0.1 1,546,412 - +0.3 1,546,417 - +0.5 1,546,417 - -1.4 1,546,417 - -1.3 1,546,418 - -0.4 1,546,422 - +0.8 1,546,445 + +1.3 1,546,490 + -0.7 1,546,491 + -0.0 1,546,491 + +1.1 1,546,498 + +0.7 1,546,500 - +0.3 1,546,515 + -1.2 1,546,542 + +1.4 1,546,555 + +0.8 1,546,557 + +1.1 1,546,559 - +0.1 1,546,586 - -0.9 1,546,602 - -1.1 1,546,651 - -1.2 1,546,653 + +0.5 1,546,653 + -0.7 1,546,653 + -0.3 1,546,653 + +0.3 1,546,653 + +0.6 1,546,653 + +0.5 1,546,664 + -1.2 1,546,676 + BWI76_RS07340 0.10 -2.5 1,546,676 + BWI76_RS07340 0.10 -0.1 1,546,676 + BWI76_RS07340 0.10 -0.2 1,546,676 + BWI76_RS07340 0.10 -0.3 1,546,684 - BWI76_RS07340 0.11 +1.8 1,546,684 - BWI76_RS07340 0.11 -0.3 1,546,695 + BWI76_RS07340 0.12 -0.9 1,546,695 + BWI76_RS07340 0.12 -0.3 1,546,695 + +0.7 1,546,697 + BWI76_RS07340 0.12 +1.1 1,546,697 + BWI76_RS07340 0.12 +0.4 1,546,697 + BWI76_RS07340 0.12 +0.0 1,546,697 + BWI76_RS07340 0.12 -0.6 1,546,697 + BWI76_RS07340 0.12 -0.1 1,546,697 + BWI76_RS07340 0.12 +1.0 1,546,698 - BWI76_RS07340 0.12 +0.3 1,546,698 - BWI76_RS07340 0.12 -0.1 1,546,702 + BWI76_RS07340 0.12 +0.7 1,546,702 + BWI76_RS07340 0.12 +0.4 1,546,702 + +2.1 1,546,702 + BWI76_RS07340 0.12 +0.5 1,546,703 - BWI76_RS07340 0.13 -0.7 1,546,703 - BWI76_RS07340 0.13 -0.7 1,546,703 - BWI76_RS07340 0.13 +0.1 1,546,705 - BWI76_RS07340 0.13 +1.7 1,546,705 - BWI76_RS07340 0.13 +1.2 1,546,705 - BWI76_RS07340 0.13 +0.7 1,546,705 - BWI76_RS07340 0.13 +1.2 1,546,709 + BWI76_RS07340 0.13 +0.6 1,546,734 + BWI76_RS07340 0.15 +1.6 1,546,735 + BWI76_RS07340 0.15 +1.1 1,546,742 + BWI76_RS07340 0.16 +1.4 1,546,742 - BWI76_RS07340 0.16 +0.1 1,546,743 - BWI76_RS07340 0.16 -2.4 1,546,777 - BWI76_RS07340 0.19 +1.1 1,546,813 + BWI76_RS07340 0.22 -1.2 1,546,824 + BWI76_RS07340 0.23 +0.6 1,546,881 - BWI76_RS07340 0.28 +1.1 1,546,929 + BWI76_RS07340 0.32 -1.4 1,546,944 + BWI76_RS07340 0.33 -1.0 1,546,947 + BWI76_RS07340 0.33 +2.6 1,546,955 - BWI76_RS07340 0.34 +0.4 1,546,972 + BWI76_RS07340 0.35 -0.1 1,547,036 + BWI76_RS07340 0.41 +1.0 1,547,228 + BWI76_RS07340 0.57 +0.4 1,547,324 + BWI76_RS07340 0.65 -0.2 1,547,644 + -0.4 1,547,732 - -0.9 1,547,734 + -1.9 1,547,778 - +0.6 1,547,778 - -0.7 1,547,788 - +1.1 1,547,855 - BWI76_RS07345 0.13 -0.3 1,547,884 + BWI76_RS07345 0.17 -0.9 1,547,929 + BWI76_RS07345 0.23 +1.1 1,547,937 - BWI76_RS07345 0.24 +1.6 1,547,943 - BWI76_RS07345 0.24 -0.8 1,547,970 + BWI76_RS07345 0.28 +1.9 1,548,030 - BWI76_RS07345 0.36 +0.9 1,548,030 - BWI76_RS07345 0.36 +0.1 1,548,067 + BWI76_RS07345 0.40 +0.0 1,548,075 - BWI76_RS07345 0.41 -0.8 1,548,075 - BWI76_RS07345 0.41 +0.5 1,548,188 + BWI76_RS07345 0.56 -0.7 1,548,201 - BWI76_RS07345 0.58 +0.2 1,548,539 + -2.5 1,548,553 + -0.4 1,548,553 + -0.8 1,548,561 - +0.7
Or see this region's nucleotide sequence