Experiment: KLEB31 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS06235 and BWI76_RS06240 are separated by 418 nucleotides BWI76_RS06240 and BWI76_RS06245 are separated by 123 nucleotides
BWI76_RS06235: BWI76_RS06235 - PAS domain-containing sensor histidine kinase, at 1,308,454 to 1,309,743
_RS06235
BWI76_RS06240: BWI76_RS06240 - branched-chain amino acid transport system 2 carrier protein BrnQ, at 1,310,162 to 1,311,481
_RS06240
BWI76_RS06245: BWI76_RS06245 - proline-specific permease ProY, at 1,311,605 to 1,312,975
_RS06245
Position (kb)
1310
1311
1312 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1309.256 kb on - strand, within BWI76_RS06235 at 1309.312 kb on + strand, within BWI76_RS06235 at 1309.374 kb on - strand, within BWI76_RS06235 at 1309.654 kb on + strand at 1309.657 kb on - strand at 1309.699 kb on + strand at 1309.814 kb on + strand at 1309.961 kb on + strand at 1310.003 kb on - strand at 1310.122 kb on - strand at 1310.139 kb on + strand at 1310.203 kb on + strand at 1310.211 kb on + strand at 1310.281 kb on + strand at 1310.281 kb on + strand at 1310.350 kb on + strand, within BWI76_RS06240 at 1310.538 kb on - strand, within BWI76_RS06240 at 1311.041 kb on + strand, within BWI76_RS06240 at 1311.058 kb on - strand, within BWI76_RS06240 at 1311.155 kb on + strand, within BWI76_RS06240 at 1311.318 kb on - strand, within BWI76_RS06240 at 1311.352 kb on + strand at 1311.352 kb on + strand at 1311.386 kb on + strand at 1311.396 kb on + strand at 1311.487 kb on + strand at 1311.490 kb on + strand at 1311.490 kb on + strand at 1311.492 kb on + strand at 1311.552 kb on + strand at 1311.681 kb on + strand at 1312.024 kb on - strand, within BWI76_RS06245 at 1312.316 kb on - strand, within BWI76_RS06245 at 1312.316 kb on - strand, within BWI76_RS06245 at 1312.472 kb on + strand, within BWI76_RS06245
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB31 MOI 1 remove 1,309,256 - BWI76_RS06235 0.62 +1.6 1,309,312 + BWI76_RS06235 0.67 +1.2 1,309,374 - BWI76_RS06235 0.71 -1.3 1,309,654 + -0.2 1,309,657 - +0.5 1,309,699 + -0.3 1,309,814 + +0.2 1,309,961 + +1.4 1,310,003 - +1.2 1,310,122 - -1.1 1,310,139 + -1.0 1,310,203 + +0.1 1,310,211 + +1.2 1,310,281 + +2.2 1,310,281 + -0.5 1,310,350 + BWI76_RS06240 0.14 +0.1 1,310,538 - BWI76_RS06240 0.28 +0.0 1,311,041 + BWI76_RS06240 0.67 +1.9 1,311,058 - BWI76_RS06240 0.68 +1.9 1,311,155 + BWI76_RS06240 0.75 +3.7 1,311,318 - BWI76_RS06240 0.88 +0.7 1,311,352 + -0.4 1,311,352 + +0.7 1,311,386 + +2.6 1,311,396 + +2.4 1,311,487 + +0.8 1,311,490 + +0.6 1,311,490 + -1.5 1,311,492 + +0.6 1,311,552 + -0.6 1,311,681 + -1.8 1,312,024 - BWI76_RS06245 0.31 -2.1 1,312,316 - BWI76_RS06245 0.52 +0.1 1,312,316 - BWI76_RS06245 0.52 -0.0 1,312,472 + BWI76_RS06245 0.63 +0.1
Or see this region's nucleotide sequence