Experiment: KLEB31 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04495 and BWI76_RS04500 are separated by 84 nucleotides BWI76_RS04500 and BWI76_RS04505 overlap by 1 nucleotides BWI76_RS04505 and BWI76_RS04510 are separated by 40 nucleotides BWI76_RS04510 and BWI76_RS04515 are separated by 4 nucleotides BWI76_RS04515 and BWI76_RS04520 are separated by 2 nucleotides
BWI76_RS04495: BWI76_RS04495 - glutathione-regulated potassium-efflux system protein KefC, at 930,808 to 932,673
_RS04495
BWI76_RS04500: BWI76_RS04500 - dihydrofolate reductase, at 932,758 to 932,871
_RS04500
BWI76_RS04505: BWI76_RS04505 - dihydrofolate reductase, at 932,871 to 933,350
_RS04505
BWI76_RS04510: BWI76_RS04510 - bis(5'-nucleosyl)-tetraphosphatase (symmetrical), at 933,391 to 934,239
_RS04510
BWI76_RS04515: BWI76_RS04515 - Co2+/Mg2+ efflux protein ApaG, at 934,244 to 934,621
_RS04515
BWI76_RS04520: BWI76_RS04520 - 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase, at 934,624 to 935,445
_RS04520
Position (kb) 933
934
935 Strain fitness (log2 ratio) -1
0
1
2
3 at 932.486 kb on - strand, within BWI76_RS04495 at 932.600 kb on + strand at 932.655 kb on + strand at 932.674 kb on + strand at 932.750 kb on + strand at 933.478 kb on + strand, within BWI76_RS04510 at 933.624 kb on + strand, within BWI76_RS04510 at 934.061 kb on - strand, within BWI76_RS04510 at 934.344 kb on - strand, within BWI76_RS04515 at 934.605 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB31 MOI 1 remove 932,486 - BWI76_RS04495 0.90 -0.2 932,600 + +2.3 932,655 + -0.3 932,674 + +1.1 932,750 + +1.5 933,478 + BWI76_RS04510 0.10 +3.1 933,624 + BWI76_RS04510 0.27 +1.0 934,061 - BWI76_RS04510 0.79 +1.4 934,344 - BWI76_RS04515 0.26 -0.4 934,605 - -1.7
Or see this region's nucleotide sequence