Experiment: KLEB31 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04415 and BWI76_RS04420 are separated by 12 nucleotides BWI76_RS04420 and BWI76_RS04425 are separated by 15 nucleotides BWI76_RS04425 and BWI76_RS04430 are separated by 160 nucleotides
BWI76_RS04415: BWI76_RS04415 - oxaloacetate decarboxylase subunit beta, at 911,208 to 912,509
_RS04415
BWI76_RS04420: BWI76_RS04420 - oxaloacetate decarboxylase, at 912,522 to 914,288
_RS04420
BWI76_RS04425: BWI76_RS04425 - oxaloacetate decarboxylase subunit gamma, at 914,304 to 914,555
_RS04425
BWI76_RS04430: BWI76_RS04430 - citrate carrier protein, at 914,716 to 916,056
_RS04430
Position (kb) 912
913
914
915 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 912.549 kb on + strand at 912.731 kb on + strand, within BWI76_RS04420 at 912.828 kb on + strand, within BWI76_RS04420 at 912.852 kb on + strand, within BWI76_RS04420 at 913.020 kb on + strand, within BWI76_RS04420 at 913.157 kb on + strand, within BWI76_RS04420 at 913.533 kb on - strand, within BWI76_RS04420 at 913.647 kb on - strand, within BWI76_RS04420 at 913.825 kb on - strand, within BWI76_RS04420 at 913.835 kb on - strand, within BWI76_RS04420 at 913.863 kb on - strand, within BWI76_RS04420 at 914.101 kb on + strand at 914.511 kb on + strand, within BWI76_RS04425 at 914.540 kb on - strand at 914.630 kb on - strand at 914.820 kb on - strand at 914.820 kb on - strand at 914.836 kb on - strand at 914.861 kb on + strand, within BWI76_RS04430 at 914.866 kb on - strand, within BWI76_RS04430 at 914.868 kb on + strand, within BWI76_RS04430 at 915.014 kb on - strand, within BWI76_RS04430 at 915.019 kb on - strand, within BWI76_RS04430 at 915.121 kb on + strand, within BWI76_RS04430 at 915.129 kb on - strand, within BWI76_RS04430 at 915.136 kb on + strand, within BWI76_RS04430 at 915.214 kb on - strand, within BWI76_RS04430 at 915.244 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB31 MOI 1 remove 912,549 + -1.0 912,731 + BWI76_RS04420 0.12 +0.6 912,828 + BWI76_RS04420 0.17 -0.3 912,852 + BWI76_RS04420 0.19 -0.7 913,020 + BWI76_RS04420 0.28 +1.0 913,157 + BWI76_RS04420 0.36 +1.0 913,533 - BWI76_RS04420 0.57 +1.6 913,647 - BWI76_RS04420 0.64 +1.8 913,825 - BWI76_RS04420 0.74 -0.2 913,835 - BWI76_RS04420 0.74 +2.3 913,863 - BWI76_RS04420 0.76 +0.1 914,101 + -1.6 914,511 + BWI76_RS04425 0.82 +0.3 914,540 - +1.6 914,630 - +0.7 914,820 - -0.1 914,820 - +2.0 914,836 - +0.5 914,861 + BWI76_RS04430 0.11 +1.2 914,866 - BWI76_RS04430 0.11 +1.4 914,868 + BWI76_RS04430 0.11 -1.2 915,014 - BWI76_RS04430 0.22 +0.2 915,019 - BWI76_RS04430 0.23 -0.3 915,121 + BWI76_RS04430 0.30 -0.7 915,129 - BWI76_RS04430 0.31 -0.8 915,136 + BWI76_RS04430 0.31 +0.3 915,214 - BWI76_RS04430 0.37 -3.5 915,244 + +1.0
Or see this region's nucleotide sequence