Experiment: KLEB31 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03120 and BWI76_RS03125 are separated by 5 nucleotides BWI76_RS03125 and BWI76_RS03130 are separated by 10 nucleotides BWI76_RS03130 and BWI76_RS03135 overlap by 11 nucleotides
BWI76_RS03120: BWI76_RS03120 - ABC transporter ATP-binding protein, at 643,322 to 644,392
_RS03120
BWI76_RS03125: BWI76_RS03125 - phosphodiesterase, at 644,398 to 645,222
_RS03125
BWI76_RS03130: BWI76_RS03130 - putative SN-glycerol-3-phosphate transport system permease, at 645,233 to 646,120
_RS03130
BWI76_RS03135: BWI76_RS03135 - sugar ABC transporter permease, at 646,110 to 646,982
_RS03135
Position (kb) 644
645
646 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 643.458 kb on - strand, within BWI76_RS03120 at 643.468 kb on - strand, within BWI76_RS03120 at 643.562 kb on + strand, within BWI76_RS03120 at 643.628 kb on + strand, within BWI76_RS03120 at 643.736 kb on + strand, within BWI76_RS03120 at 643.744 kb on - strand, within BWI76_RS03120 at 643.827 kb on - strand, within BWI76_RS03120 at 644.049 kb on + strand, within BWI76_RS03120 at 644.247 kb on - strand, within BWI76_RS03120 at 644.269 kb on - strand, within BWI76_RS03120 at 644.616 kb on + strand at 644.699 kb on - strand, within BWI76_RS03125 at 644.908 kb on - strand, within BWI76_RS03125 at 644.965 kb on - strand, within BWI76_RS03125 at 645.084 kb on + strand, within BWI76_RS03125 at 645.103 kb on + strand, within BWI76_RS03125 at 645.103 kb on + strand, within BWI76_RS03125 at 645.105 kb on + strand, within BWI76_RS03125 at 645.462 kb on - strand, within BWI76_RS03130 at 645.476 kb on + strand, within BWI76_RS03130 at 645.484 kb on - strand, within BWI76_RS03130 at 645.484 kb on - strand, within BWI76_RS03130 at 645.523 kb on - strand, within BWI76_RS03130 at 645.634 kb on + strand, within BWI76_RS03130 at 645.801 kb on + strand, within BWI76_RS03130 at 645.984 kb on + strand, within BWI76_RS03130 at 646.016 kb on + strand, within BWI76_RS03130 at 646.024 kb on - strand, within BWI76_RS03130
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB31 MOI 1 remove 643,458 - BWI76_RS03120 0.13 -0.7 643,468 - BWI76_RS03120 0.14 +0.6 643,562 + BWI76_RS03120 0.22 +0.8 643,628 + BWI76_RS03120 0.29 +0.5 643,736 + BWI76_RS03120 0.39 +0.7 643,744 - BWI76_RS03120 0.39 -0.9 643,827 - BWI76_RS03120 0.47 +0.6 644,049 + BWI76_RS03120 0.68 -0.1 644,247 - BWI76_RS03120 0.86 -0.8 644,269 - BWI76_RS03120 0.88 +1.8 644,616 + +4.3 644,699 - BWI76_RS03125 0.36 -0.6 644,908 - BWI76_RS03125 0.62 -0.8 644,965 - BWI76_RS03125 0.69 -0.2 645,084 + BWI76_RS03125 0.83 -0.6 645,103 + BWI76_RS03125 0.85 -1.8 645,103 + BWI76_RS03125 0.85 +0.8 645,105 + BWI76_RS03125 0.86 -0.7 645,462 - BWI76_RS03130 0.26 -0.1 645,476 + BWI76_RS03130 0.27 +1.1 645,484 - BWI76_RS03130 0.28 +0.1 645,484 - BWI76_RS03130 0.28 +0.4 645,523 - BWI76_RS03130 0.33 -1.2 645,634 + BWI76_RS03130 0.45 +0.4 645,801 + BWI76_RS03130 0.64 +1.2 645,984 + BWI76_RS03130 0.85 -0.7 646,016 + BWI76_RS03130 0.88 -1.6 646,024 - BWI76_RS03130 0.89 +2.6
Or see this region's nucleotide sequence