Experiment: KLEB31 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02590 and BWI76_RS02595 overlap by 10 nucleotides BWI76_RS02595 and BWI76_RS02600 are separated by 138 nucleotides BWI76_RS02600 and BWI76_RS02605 are separated by 176 nucleotides
BWI76_RS02590: BWI76_RS02590 - isovaleryl-CoA dehydrogenase, at 530,505 to 532,142
_RS02590
BWI76_RS02595: BWI76_RS02595 - hypothetical protein, at 532,133 to 532,408
_RS02595
BWI76_RS02600: BWI76_RS02600 - biofilm peroxide resistance protein BsmA, at 532,547 to 532,876
_RS02600
BWI76_RS02605: BWI76_RS02605 - esterase, at 533,053 to 533,769
_RS02605
Position (kb)
532
533 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 531.256 kb on - strand, within BWI76_RS02590 at 531.261 kb on + strand, within BWI76_RS02590 at 531.264 kb on - strand, within BWI76_RS02590 at 531.351 kb on + strand, within BWI76_RS02590 at 531.419 kb on - strand, within BWI76_RS02590 at 531.421 kb on + strand, within BWI76_RS02590 at 531.448 kb on - strand, within BWI76_RS02590 at 531.458 kb on + strand, within BWI76_RS02590 at 531.522 kb on + strand, within BWI76_RS02590 at 531.598 kb on - strand, within BWI76_RS02590 at 531.709 kb on + strand, within BWI76_RS02590 at 531.737 kb on + strand, within BWI76_RS02590 at 531.942 kb on - strand, within BWI76_RS02590 at 531.996 kb on + strand at 532.143 kb on + strand at 532.195 kb on - strand, within BWI76_RS02595 at 532.244 kb on - strand, within BWI76_RS02595 at 532.246 kb on + strand, within BWI76_RS02595 at 532.370 kb on + strand, within BWI76_RS02595 at 532.437 kb on - strand at 532.437 kb on - strand at 532.437 kb on - strand at 532.538 kb on - strand at 532.596 kb on - strand, within BWI76_RS02600 at 532.644 kb on + strand, within BWI76_RS02600 at 532.652 kb on - strand, within BWI76_RS02600 at 532.690 kb on - strand, within BWI76_RS02600 at 532.757 kb on + strand, within BWI76_RS02600 at 532.789 kb on + strand, within BWI76_RS02600 at 532.795 kb on - strand, within BWI76_RS02600 at 532.834 kb on + strand, within BWI76_RS02600 at 532.834 kb on + strand, within BWI76_RS02600 at 532.834 kb on + strand, within BWI76_RS02600 at 532.842 kb on - strand, within BWI76_RS02600 at 532.842 kb on - strand, within BWI76_RS02600 at 532.842 kb on - strand, within BWI76_RS02600 at 532.878 kb on + strand at 532.886 kb on - strand at 533.068 kb on - strand at 533.165 kb on + strand, within BWI76_RS02605 at 533.173 kb on - strand, within BWI76_RS02605 at 533.173 kb on - strand, within BWI76_RS02605 at 533.173 kb on - strand, within BWI76_RS02605 at 533.176 kb on + strand, within BWI76_RS02605 at 533.239 kb on + strand, within BWI76_RS02605 at 533.400 kb on + strand, within BWI76_RS02605
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB31 MOI 1 remove 531,256 - BWI76_RS02590 0.46 +0.4 531,261 + BWI76_RS02590 0.46 -0.0 531,264 - BWI76_RS02590 0.46 +0.7 531,351 + BWI76_RS02590 0.52 -1.2 531,419 - BWI76_RS02590 0.56 +0.2 531,421 + BWI76_RS02590 0.56 -1.0 531,448 - BWI76_RS02590 0.58 -0.4 531,458 + BWI76_RS02590 0.58 +0.5 531,522 + BWI76_RS02590 0.62 -1.1 531,598 - BWI76_RS02590 0.67 +0.4 531,709 + BWI76_RS02590 0.74 +0.1 531,737 + BWI76_RS02590 0.75 -0.6 531,942 - BWI76_RS02590 0.88 -2.3 531,996 + +1.8 532,143 + +1.0 532,195 - BWI76_RS02595 0.22 +1.4 532,244 - BWI76_RS02595 0.40 -0.1 532,246 + BWI76_RS02595 0.41 -1.7 532,370 + BWI76_RS02595 0.86 -0.5 532,437 - +1.3 532,437 - -0.5 532,437 - -1.4 532,538 - -1.2 532,596 - BWI76_RS02600 0.15 +1.6 532,644 + BWI76_RS02600 0.29 -1.9 532,652 - BWI76_RS02600 0.32 -1.6 532,690 - BWI76_RS02600 0.43 -0.4 532,757 + BWI76_RS02600 0.64 -1.7 532,789 + BWI76_RS02600 0.73 +0.1 532,795 - BWI76_RS02600 0.75 +0.4 532,834 + BWI76_RS02600 0.87 -1.0 532,834 + BWI76_RS02600 0.87 -0.8 532,834 + BWI76_RS02600 0.87 -2.8 532,842 - BWI76_RS02600 0.89 +0.7 532,842 - BWI76_RS02600 0.89 -0.0 532,842 - BWI76_RS02600 0.89 +0.7 532,878 + -0.4 532,886 - +0.3 533,068 - -0.9 533,165 + BWI76_RS02605 0.16 -2.3 533,173 - BWI76_RS02605 0.17 -0.2 533,173 - BWI76_RS02605 0.17 -1.4 533,173 - BWI76_RS02605 0.17 -1.4 533,176 + BWI76_RS02605 0.17 +1.1 533,239 + BWI76_RS02605 0.26 +2.0 533,400 + BWI76_RS02605 0.48 -0.9
Or see this region's nucleotide sequence