Experiment: KLEB31 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01975 and BWI76_RS01980 are separated by 319 nucleotides BWI76_RS01980 and BWI76_RS01985 are separated by 145 nucleotides BWI76_RS01985 and BWI76_RS01990 are separated by 544 nucleotides
BWI76_RS01975: BWI76_RS01975 - outer membrane protein assembly factor BamA, at 405,031 to 407,454
_RS01975
BWI76_RS01980: BWI76_RS01980 - anti-adapter protein IraM, at 407,774 to 408,163
_RS01980
BWI76_RS01985: BWI76_RS01985 - membrane protein, at 408,309 to 408,590
_RS01985
BWI76_RS01990: BWI76_RS01990 - cyclic diguanylate phosphodiesterase, at 409,135 to 410,739
_RS01990
Position (kb) 408
409 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 407.317 kb on + strand at 407.317 kb on + strand at 407.317 kb on + strand at 407.325 kb on - strand at 407.325 kb on - strand at 407.325 kb on - strand at 407.336 kb on + strand at 407.338 kb on + strand at 407.341 kb on - strand at 407.346 kb on + strand at 407.346 kb on - strand at 407.354 kb on - strand at 407.356 kb on + strand at 407.356 kb on + strand at 407.357 kb on + strand at 407.368 kb on + strand at 407.370 kb on + strand at 407.376 kb on + strand at 407.376 kb on + strand at 407.376 kb on + strand at 407.384 kb on - strand at 407.409 kb on + strand at 407.409 kb on + strand at 407.409 kb on + strand at 407.409 kb on + strand at 407.409 kb on + strand at 407.409 kb on + strand at 407.409 kb on + strand at 407.409 kb on + strand at 407.409 kb on + strand at 407.409 kb on + strand at 407.409 kb on + strand at 407.409 kb on + strand at 407.409 kb on + strand at 407.409 kb on + strand at 407.414 kb on + strand at 407.414 kb on + strand at 407.414 kb on + strand at 407.414 kb on + strand at 407.414 kb on + strand at 407.416 kb on + strand at 407.417 kb on - strand at 407.417 kb on - strand at 407.417 kb on - strand at 407.417 kb on - strand at 407.417 kb on - strand at 407.417 kb on - strand at 407.417 kb on - strand at 407.417 kb on - strand at 407.417 kb on - strand at 407.417 kb on - strand at 407.417 kb on - strand at 407.417 kb on - strand at 407.422 kb on - strand at 407.422 kb on - strand at 407.422 kb on - strand at 407.422 kb on - strand at 407.422 kb on - strand at 407.424 kb on + strand at 407.424 kb on + strand at 407.468 kb on - strand at 407.471 kb on + strand at 407.476 kb on + strand at 407.476 kb on + strand at 407.479 kb on - strand at 407.482 kb on - strand at 407.507 kb on + strand at 407.507 kb on + strand at 407.507 kb on + strand at 407.507 kb on + strand at 407.507 kb on + strand at 407.507 kb on + strand at 407.507 kb on + strand at 407.507 kb on + strand at 407.507 kb on + strand at 407.507 kb on + strand at 407.507 kb on + strand at 407.507 kb on + strand at 407.507 kb on + strand at 407.507 kb on + strand at 407.515 kb on - strand at 407.515 kb on - strand at 407.515 kb on - strand at 407.520 kb on + strand at 407.520 kb on + strand at 407.520 kb on + strand at 407.520 kb on + strand at 407.527 kb on + strand at 407.527 kb on + strand at 407.527 kb on + strand at 407.528 kb on - strand at 407.528 kb on - strand at 407.528 kb on - strand at 407.530 kb on - strand at 407.538 kb on - strand at 407.575 kb on + strand at 407.583 kb on - strand at 407.583 kb on - strand at 407.594 kb on - strand at 407.639 kb on + strand at 407.639 kb on + strand at 407.639 kb on + strand at 407.647 kb on - strand at 407.651 kb on - strand at 407.653 kb on - strand at 407.655 kb on + strand at 407.655 kb on + strand at 407.655 kb on + strand at 407.655 kb on + strand at 407.663 kb on - strand at 407.663 kb on - strand at 407.663 kb on - strand at 407.663 kb on - strand at 407.663 kb on - strand at 407.663 kb on - strand at 407.663 kb on - strand at 407.663 kb on - strand at 407.710 kb on - strand at 407.714 kb on + strand at 407.715 kb on - strand at 407.719 kb on + strand at 407.722 kb on - strand at 407.727 kb on - strand at 407.729 kb on + strand at 407.731 kb on + strand at 407.737 kb on - strand at 407.755 kb on - strand at 407.760 kb on - strand at 407.784 kb on - strand at 407.786 kb on + strand at 407.786 kb on + strand at 407.794 kb on - strand at 407.794 kb on - strand at 407.794 kb on - strand at 407.794 kb on - strand at 407.794 kb on - strand at 407.795 kb on + strand at 407.795 kb on + strand at 407.795 kb on + strand at 407.810 kb on + strand at 407.840 kb on + strand, within BWI76_RS01980 at 407.849 kb on - strand, within BWI76_RS01980 at 407.909 kb on + strand, within BWI76_RS01980 at 407.919 kb on + strand, within BWI76_RS01980 at 407.919 kb on + strand, within BWI76_RS01980 at 407.927 kb on + strand, within BWI76_RS01980 at 407.935 kb on - strand, within BWI76_RS01980 at 407.952 kb on - strand, within BWI76_RS01980 at 407.960 kb on + strand, within BWI76_RS01980 at 407.960 kb on + strand, within BWI76_RS01980 at 407.960 kb on - strand, within BWI76_RS01980 at 407.982 kb on + strand, within BWI76_RS01980 at 407.990 kb on - strand, within BWI76_RS01980 at 407.994 kb on + strand, within BWI76_RS01980 at 407.998 kb on - strand, within BWI76_RS01980 at 408.000 kb on + strand, within BWI76_RS01980 at 408.000 kb on - strand, within BWI76_RS01980 at 408.013 kb on + strand, within BWI76_RS01980 at 408.016 kb on + strand, within BWI76_RS01980 at 408.045 kb on + strand, within BWI76_RS01980 at 408.052 kb on + strand, within BWI76_RS01980 at 408.052 kb on + strand, within BWI76_RS01980 at 408.052 kb on + strand, within BWI76_RS01980 at 408.054 kb on + strand, within BWI76_RS01980 at 408.060 kb on - strand, within BWI76_RS01980 at 408.060 kb on - strand, within BWI76_RS01980 at 408.073 kb on - strand, within BWI76_RS01980 at 408.113 kb on + strand, within BWI76_RS01980 at 408.113 kb on + strand, within BWI76_RS01980 at 408.113 kb on + strand, within BWI76_RS01980 at 408.121 kb on - strand, within BWI76_RS01980 at 408.121 kb on - strand, within BWI76_RS01980 at 408.126 kb on + strand at 408.134 kb on - strand at 408.166 kb on - strand at 408.200 kb on - strand at 408.208 kb on + strand at 408.208 kb on + strand at 408.247 kb on + strand at 408.255 kb on - strand at 408.255 kb on - strand at 408.265 kb on - strand at 408.283 kb on - strand at 408.394 kb on + strand, within BWI76_RS01985 at 408.449 kb on + strand, within BWI76_RS01985 at 408.449 kb on + strand, within BWI76_RS01985 at 408.457 kb on - strand, within BWI76_RS01985 at 408.457 kb on - strand, within BWI76_RS01985 at 408.470 kb on + strand, within BWI76_RS01985 at 408.498 kb on - strand at 408.528 kb on + strand, within BWI76_RS01985 at 408.528 kb on + strand, within BWI76_RS01985 at 408.528 kb on + strand, within BWI76_RS01985 at 408.541 kb on - strand, within BWI76_RS01985 at 408.546 kb on - strand, within BWI76_RS01985 at 408.553 kb on - strand, within BWI76_RS01985 at 408.563 kb on - strand at 408.592 kb on + strand at 408.654 kb on + strand at 408.772 kb on - strand at 408.880 kb on + strand at 408.939 kb on + strand at 409.009 kb on - strand at 409.030 kb on + strand at 409.035 kb on + strand at 409.038 kb on - strand at 409.043 kb on - strand at 409.049 kb on + strand at 409.049 kb on + strand at 409.062 kb on - strand at 409.062 kb on - strand at 409.088 kb on + strand at 409.088 kb on + strand at 409.088 kb on + strand at 409.088 kb on + strand at 409.096 kb on - strand at 409.102 kb on + strand at 409.102 kb on + strand at 409.102 kb on + strand at 409.102 kb on + strand at 409.110 kb on - strand at 409.110 kb on - strand at 409.110 kb on - strand at 409.110 kb on - strand at 409.110 kb on - strand at 409.137 kb on + strand at 409.180 kb on + strand at 409.180 kb on + strand at 409.184 kb on + strand at 409.194 kb on - strand at 409.204 kb on + strand at 409.217 kb on + strand at 409.222 kb on + strand at 409.235 kb on - strand at 409.329 kb on - strand, within BWI76_RS01990 at 409.374 kb on + strand, within BWI76_RS01990 at 409.381 kb on - strand, within BWI76_RS01990 at 409.404 kb on + strand, within BWI76_RS01990 at 409.412 kb on - strand, within BWI76_RS01990 at 409.412 kb on - strand, within BWI76_RS01990 at 409.417 kb on + strand, within BWI76_RS01990 at 409.571 kb on + strand, within BWI76_RS01990 at 409.571 kb on + strand, within BWI76_RS01990 at 409.579 kb on - strand, within BWI76_RS01990 at 409.579 kb on - strand, within BWI76_RS01990 at 409.579 kb on - strand, within BWI76_RS01990 at 409.579 kb on - strand, within BWI76_RS01990 at 409.579 kb on - strand, within BWI76_RS01990
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB31 MOI 1 remove 407,317 + -0.3 407,317 + +3.0 407,317 + -0.2 407,325 - +0.6 407,325 - -1.3 407,325 - -1.2 407,336 + -0.6 407,338 + +1.0 407,341 - -1.6 407,346 + -0.8 407,346 - +1.0 407,354 - +0.1 407,356 + -0.7 407,356 + +2.5 407,357 + -1.8 407,368 + -0.7 407,370 + +0.1 407,376 + +0.2 407,376 + -0.2 407,376 + -1.8 407,384 - +1.7 407,409 + -1.2 407,409 + +1.1 407,409 + +0.3 407,409 + -0.3 407,409 + +0.3 407,409 + -0.9 407,409 + -0.4 407,409 + +0.6 407,409 + +1.2 407,409 + -1.6 407,409 + +3.4 407,409 + -0.5 407,409 + -0.6 407,409 + +0.7 407,414 + -1.3 407,414 + +1.3 407,414 + +0.1 407,414 + -0.4 407,414 + +0.5 407,416 + +2.2 407,417 - -2.2 407,417 - +0.7 407,417 - +0.7 407,417 - +0.2 407,417 - -0.1 407,417 - -1.0 407,417 - +2.0 407,417 - -0.3 407,417 - +1.4 407,417 - +0.7 407,417 - +2.7 407,417 - -2.5 407,422 - -0.5 407,422 - -0.6 407,422 - -1.4 407,422 - +4.0 407,422 - -0.6 407,424 + +0.3 407,424 + +0.4 407,468 - +2.6 407,471 + -0.8 407,476 + +0.4 407,476 + -1.8 407,479 - -1.7 407,482 - +1.8 407,507 + +0.1 407,507 + -1.4 407,507 + +1.9 407,507 + +1.2 407,507 + +0.6 407,507 + +1.0 407,507 + -1.3 407,507 + +1.1 407,507 + +0.4 407,507 + +1.0 407,507 + -0.1 407,507 + +0.0 407,507 + +1.2 407,507 + +0.9 407,515 - +1.5 407,515 - -0.7 407,515 - +0.1 407,520 + +1.6 407,520 + -0.0 407,520 + +0.1 407,520 + -1.8 407,527 + +1.9 407,527 + +1.7 407,527 + -1.7 407,528 - +1.1 407,528 - +1.3 407,528 - -1.8 407,530 - -2.0 407,538 - -1.0 407,575 + +1.0 407,583 - -0.6 407,583 - +1.7 407,594 - -0.1 407,639 + +0.2 407,639 + -2.1 407,639 + -0.6 407,647 - -2.3 407,651 - -1.5 407,653 - -1.0 407,655 + -0.3 407,655 + +3.0 407,655 + +1.5 407,655 + +0.6 407,663 - -0.8 407,663 - -1.6 407,663 - +1.7 407,663 - +1.4 407,663 - +0.9 407,663 - -0.6 407,663 - -2.3 407,663 - -1.3 407,710 - +0.7 407,714 + -0.8 407,715 - +0.7 407,719 + -1.8 407,722 - -1.8 407,727 - +0.2 407,729 + -1.0 407,731 + +1.2 407,737 - -1.1 407,755 - +1.6 407,760 - +0.0 407,784 - -1.2 407,786 + -0.0 407,786 + -2.6 407,794 - +1.4 407,794 - -1.2 407,794 - +1.0 407,794 - +2.0 407,794 - -0.0 407,795 + -0.6 407,795 + -0.5 407,795 + +0.9 407,810 + +1.0 407,840 + BWI76_RS01980 0.17 -1.3 407,849 - BWI76_RS01980 0.19 +0.8 407,909 + BWI76_RS01980 0.35 -1.7 407,919 + BWI76_RS01980 0.37 +1.0 407,919 + BWI76_RS01980 0.37 +0.2 407,927 + BWI76_RS01980 0.39 -1.1 407,935 - BWI76_RS01980 0.41 +0.4 407,952 - BWI76_RS01980 0.46 -0.7 407,960 + BWI76_RS01980 0.48 -0.1 407,960 + BWI76_RS01980 0.48 +1.8 407,960 - BWI76_RS01980 0.48 -1.0 407,982 + BWI76_RS01980 0.53 -0.6 407,990 - BWI76_RS01980 0.55 +1.2 407,994 + BWI76_RS01980 0.56 +0.3 407,998 - BWI76_RS01980 0.57 -2.7 408,000 + BWI76_RS01980 0.58 -0.4 408,000 - BWI76_RS01980 0.58 -0.2 408,013 + BWI76_RS01980 0.61 +0.9 408,016 + BWI76_RS01980 0.62 +1.0 408,045 + BWI76_RS01980 0.69 +0.0 408,052 + BWI76_RS01980 0.71 +0.2 408,052 + BWI76_RS01980 0.71 -1.0 408,052 + BWI76_RS01980 0.71 -0.5 408,054 + BWI76_RS01980 0.72 -0.7 408,060 - BWI76_RS01980 0.73 -1.8 408,060 - BWI76_RS01980 0.73 +0.6 408,073 - BWI76_RS01980 0.77 +0.6 408,113 + BWI76_RS01980 0.87 +0.9 408,113 + BWI76_RS01980 0.87 -0.7 408,113 + BWI76_RS01980 0.87 -0.2 408,121 - BWI76_RS01980 0.89 -1.2 408,121 - BWI76_RS01980 0.89 -0.2 408,126 + +0.6 408,134 - -0.8 408,166 - +1.6 408,200 - +0.5 408,208 + -0.0 408,208 + -0.4 408,247 + +0.6 408,255 - +0.2 408,255 - -1.1 408,265 - -0.2 408,283 - +0.1 408,394 + BWI76_RS01985 0.30 -0.7 408,449 + BWI76_RS01985 0.50 -0.8 408,449 + BWI76_RS01985 0.50 -0.5 408,457 - BWI76_RS01985 0.52 +0.5 408,457 - BWI76_RS01985 0.52 +0.1 408,470 + BWI76_RS01985 0.57 -0.4 408,498 - +1.0 408,528 + BWI76_RS01985 0.78 -1.3 408,528 + BWI76_RS01985 0.78 -0.9 408,528 + BWI76_RS01985 0.78 -0.6 408,541 - BWI76_RS01985 0.82 -0.6 408,546 - BWI76_RS01985 0.84 +1.0 408,553 - BWI76_RS01985 0.87 -1.2 408,563 - -0.3 408,592 + +1.2 408,654 + +1.0 408,772 - -0.0 408,880 + +1.3 408,939 + +0.2 409,009 - -1.3 409,030 + +0.3 409,035 + +0.8 409,038 - +2.6 409,043 - -0.7 409,049 + -0.4 409,049 + +0.3 409,062 - +1.2 409,062 - -1.3 409,088 + +0.8 409,088 + -0.5 409,088 + -0.6 409,088 + -0.2 409,096 - -0.0 409,102 + +0.5 409,102 + +0.8 409,102 + +0.2 409,102 + +0.9 409,110 - +0.5 409,110 - -0.0 409,110 - -1.3 409,110 - -1.0 409,110 - -1.7 409,137 + +0.1 409,180 + +1.7 409,180 + +2.5 409,184 + +0.5 409,194 - +1.9 409,204 + +0.1 409,217 + -0.2 409,222 + -0.5 409,235 - +0.8 409,329 - BWI76_RS01990 0.12 +0.2 409,374 + BWI76_RS01990 0.15 +2.3 409,381 - BWI76_RS01990 0.15 -0.9 409,404 + BWI76_RS01990 0.17 +0.4 409,412 - BWI76_RS01990 0.17 -1.7 409,412 - BWI76_RS01990 0.17 +0.6 409,417 + BWI76_RS01990 0.18 +0.6 409,571 + BWI76_RS01990 0.27 +0.6 409,571 + BWI76_RS01990 0.27 -0.6 409,579 - BWI76_RS01990 0.28 -3.5 409,579 - BWI76_RS01990 0.28 -0.2 409,579 - BWI76_RS01990 0.28 +0.8 409,579 - BWI76_RS01990 0.28 -0.3 409,579 - BWI76_RS01990 0.28 -0.4
Or see this region's nucleotide sequence