Experiment: KLEB31 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01830 and BWI76_RS01835 are separated by 27 nucleotides BWI76_RS01835 and BWI76_RS01840 are separated by 55 nucleotides BWI76_RS01840 and BWI76_RS01845 are separated by 98 nucleotides
BWI76_RS01830: BWI76_RS01830 - maltose ABC transporter, substrate-binding component MalE (from data) , at 373,299 to 374,489
_RS01830
BWI76_RS01835: BWI76_RS01835 - chemotaxis protein CheW, at 374,517 to 374,804
_RS01835
BWI76_RS01840: BWI76_RS01840 - maltose ABC transporter, ATPase component (MalK) (from data) , at 374,860 to 375,969
_RS01840
BWI76_RS01845: BWI76_RS01845 - maltoporin, at 376,068 to 377,354
_RS01845
Position (kb)
374
375
376 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 373.860 kb on + strand, within BWI76_RS01830 at 373.969 kb on - strand, within BWI76_RS01830 at 373.978 kb on + strand, within BWI76_RS01830 at 374.161 kb on + strand, within BWI76_RS01830 at 374.580 kb on + strand, within BWI76_RS01835 at 374.586 kb on + strand, within BWI76_RS01835 at 374.594 kb on - strand, within BWI76_RS01835 at 374.642 kb on + strand, within BWI76_RS01835 at 374.789 kb on + strand at 375.168 kb on - strand, within BWI76_RS01840 at 375.681 kb on + strand, within BWI76_RS01840 at 375.924 kb on + strand at 375.995 kb on + strand at 376.139 kb on + strand at 376.347 kb on - strand, within BWI76_RS01845 at 376.375 kb on + strand, within BWI76_RS01845 at 376.547 kb on - strand, within BWI76_RS01845 at 376.708 kb on + strand, within BWI76_RS01845 at 376.708 kb on + strand, within BWI76_RS01845 at 376.733 kb on - strand, within BWI76_RS01845 at 376.786 kb on + strand, within BWI76_RS01845 at 376.932 kb on + strand, within BWI76_RS01845
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB31 MOI 1 remove 373,860 + BWI76_RS01830 0.47 +0.3 373,969 - BWI76_RS01830 0.56 -1.1 373,978 + BWI76_RS01830 0.57 -2.9 374,161 + BWI76_RS01830 0.72 +2.1 374,580 + BWI76_RS01835 0.22 +0.9 374,586 + BWI76_RS01835 0.24 +0.3 374,594 - BWI76_RS01835 0.27 -1.7 374,642 + BWI76_RS01835 0.43 -1.3 374,789 + +0.2 375,168 - BWI76_RS01840 0.28 +2.1 375,681 + BWI76_RS01840 0.74 +0.1 375,924 + -1.0 375,995 + +1.1 376,139 + -0.6 376,347 - BWI76_RS01845 0.22 -0.3 376,375 + BWI76_RS01845 0.24 +0.6 376,547 - BWI76_RS01845 0.37 +0.0 376,708 + BWI76_RS01845 0.50 +0.5 376,708 + BWI76_RS01845 0.50 -0.5 376,733 - BWI76_RS01845 0.52 -2.6 376,786 + BWI76_RS01845 0.56 +0.5 376,932 + BWI76_RS01845 0.67 +0.3
Or see this region's nucleotide sequence